Human RANK Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-RANK (SKU#: LTP0281)

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Price:

$996.00

Description

RANK (receptor activator of NFκB), also known as CD265 and ODFR, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF11A). RANK contains 4 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). RANK is ubiquitously expressed with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. TRANCE (TNFrelated activationinduced cytokines), also known as OPGL (osteoprotegerin ligand) and ODF (osteoclast differentiation factor), is the ligand for RANK (RANKL). RANK and RANKL are important regulators of the interaction between T cells and dendritic cells for the enhancement of T cell growth and dendritic cell function. They are also essential mediators for osteoclast and lymph node development. Mutations in RANK are associated with familial expansile osteolysis (FEO), Paget disease of bone type 2 (PDB2), and osteopetrosis autosomal recessive type 7 (OPTB7). FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. OPTB7 is characterized by abnormally dense bone, due to defective resorption of immature bone. Soluble RANK is able to block RANKL-induced biological activity. RANK can interact with various TRAF family proteins, through which this receptor induces the activation of NF-κB and JNK pathways.

Gene Symbol: TNFRSF11A; CD265; FEO; OFE; ODFR; OSTS; PDB2; OPTB7; TRANCER; LOH18CR1

NCBI Gene ID: 8792

Uniprot Entry: Q9Y6Q6

Construct Details: Full length human RANK gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Sequence:

ATGGCCCCGCGCGCCCGGCGGCGCCGCCCGCTGTTCGCGCTGCTGCTGCTCTGCGCGCTGCTCGCCCGGCTGCAGGTGGC

TTTGCAGATCGCTCCTCCATGTACCAGTGAGAAGCATTATGAGCATCTGGGACGGTGCTGTAACAAATGTGAACCAGGAA

AGTACATGTCTTCTAAATGCACTACTACCTCTGACAGTGTATGTCTGCCCTGTGGCCCGGATGAATACTTGGATAGCTGG

AATGAAGAAGATAAATGCTTGCTGCATAAAGTTTGTGATACAGGCAAGGCCCTGGTGGCCGTGGTCGCCGGCAACAGCAC

GACCCCCCGGCGCTGCGCGTGCACGGCTGGGTACCACTGGAGCCAGGACTGCGAGTGCTGCCGCCGCAACACCGAGTGCG

CGCCGGGCCTGGGCGCCCAGCACCCGTTGCAGCTCAACAAGGACACAGTGTGCAAACCTTGCCTTGCAGGCTACTTCTCT

GATGCCTTTTCCTCCACGGACAAATGCAGACCCTGGACCAACTGTACCTTCCTTGGAAAGAGAGTAGAACATCATGGGAC

AGAGAAATCCGATGCGGTTTGCAGTTCTTCTCTGCCAGCTAGAAAACCACCAAATGAACCCCATGTTTACTTGCCCGGTT

TAATAATTCTGCTTCTCTTCGCGTCTGTGGCCCTGGTGGCTGCCATCATCTTTGGCGTTTGCTATAGGAAAAAAGGGAAA

GCACTCACAGCTAATTTGTGGCACTGGATCAATGAGGCTTGTGGCCGCCTAAGTGGAGATAAGGAGTCCTCAGGTGACAG

TTGTGTCAGTACACACACGGCAAACTTTGGTCAGCAGGGAGCATGTGAAGGTGTCTTACTGCTGACTCTGGAGGAGAAGA

CATTTCCAGAAGATATGTGCTACCCAGATCAAGGTGGTGTCTGTCAGGGCACATGTGTAGGAGGTGGTCCCTACGCACAA

GGCGAAGATGCCAGGATGCTCTCATTGGTCAGCAAGACCGAGATAGAGGAAGACAGCTTCAGACAGATGCCCACAGAAGA

TGAATACATGGACAGGCCCTCCCAGCCCACAGACCAGTTACTGTTCCTCACTGAGCCTGGAAGCAAATCCACACCTCCTT

TCTCTGAACCCCTGGAGGTGGGGGAGAATGACAGTTTAAGCCAGTGCTTCACGGGGACACAGAGCACAGTGGGTTCAGAA

AGCTGCAACTGCACTGAGCCCCTGTGCAGGACTGATTGGACTCCCATGTCCTCTGAAAACTACTTGCAAAAAGAGGTGGA

CAGTGGCCATTGCCCGCACTGGGCAGCCAGCCCCAGCCCCAACTGGGCAGATGTCTGCACAGGCTGCCGGAACCCTCCTG

GGGAGGACTGTGAACCCCTCGTGGGTTCCCCAAAACGTGGACCCTTGCCCCAGTGCGCCTATGGCATGGGCCTTCCCCCT

GAAGAAGAAGCCAGCAGGACGGAGGCCAGAGACCAGCCCGAGGATGGGGCTGATGGGAGGCTCCCAAGCTCAGCGAGGGC

AGGTGCCGGGTCTGGAAGCTCCCCTGGTGGCCAGTCCCCTGCATCTGGAAATGTGACTGGAAACAGTAACTCCACGTTCA

TCTCCAGCGGGCAGGTGATGAACTTCAAGGGCGACATCATCGTGGTCTACGTCAGCCAGACCTCGCAGGAGGGCGCGGCG

GCGGCTGCGGAGCCCATGGGCCGCCCGGTGCAGGAGGAGACCCTGGCGCGCCGAGACTCCTTCGCGGGGAACGGCCCGCG

CTTCCCGGACCCGTGCGGCGGCCCCGAGGGGCTGCGGGAGCCGGAGAAGGCCTCGAGGCCGGTGCAGGAGCAAGGCGGGG

CCAAGGCTTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

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4. Nat. Genet. 24:45 (2000).

5. Am. J. Hum. Genet. 83:64 (2008).

Product datasheet (pdf) can be downloaded here: LTP0281-PDS.pdf