Human ROR1 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

ROR1 (receptor tyrosine kinase-like orphan receptor 1), also known as NTRKR1 (neurotrophic tyrosine kinase, receptor-related 1), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR1 is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with various hematological malignancies of both lymphoid and myeloid origins as well as in melanoma. ROR1 is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. ROR1 modulates neurite growth in the central nervous system. ROR1 may play a role in the survival of melanoma cells and regulate EMT and tumor metastasis.

 

 

Gene Symbol: NTRKR1; dJ537F10.1

 

NCBI Gene ID: 4919

 

Uniprot Entry: Q01973

 

Construct Details: Full length human ROR1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGCACCGGCCGCGCCGCCGCGGGACGCGCCCGCCGCTCCTGGCGCTGCTGGCCGCGCTGCTGCTGGCCGCACGCGGGGC

TGCTGCCCAAGAAACAGAGCTGTCAGTCAGTGCTGAATTAGTGCCTACCTCATCATGGAACATCTCAAGTGAACTCAACA

AAGATTCTTACCTGACCCTCGATGAACCAATGAATAACATCACCACGTCTCTGGGCCAGACAGCAGAACTGCACTGCAAA

GTCTCTGGGAATCCACCTCCCACCATCCGCTGGTTCAAAAATGATGCTCCTGTGGTCCAGGAGCCCCGGAGGCTCTCCTT

TCGGTCCACCATCTATGGCTCTCGGCTGCGGATTAGAAACCTCGACACCACAGACACAGGCTACTTCCAGTGCGTGGCAA

CAAACGGCAAGGAGGTGGTTTCTTCCACTGGAGTCTTGTTTGTCAAGTTTGGCCCCCCTCCCACTGCAAGTCCAGGATAC

TCAGATGAGTATGAAGAAGATGGATTCTGTCAGCCATACAGAGGGATTGCATGTGCAAGATTTATTGGCAACCGCACCGT

CTATATGGAGTCTTTGCACATGCAAGGGGAAATAGAAAATCAGATCACAGCTGCCTTCACTATGATTGGCACTTCCAGTC

ACTTATCTGATAAGTGTTCTCAGTTCGCCATTCCTTCCCTGTGCCACTATGCCTTCCCGTACTGCGATGAAACTTCATCC

GTCCCAAAGCCCCGTGACTTGTGTCGCGATGAATGTGAAATCCTGGAGAATGTCCTGTGTCAAACAGAGTACATTTTTGC

AAGATCAAATCCCATGATTCTGATGAGGCTGAAACTGCCAAACTGTGAAGATCTCCCCCAGCCAGAGAGCCCAGAAGCTG

CGAACTGTATCCGGATTGGAATTCCCATGGCAGATCCTATAAATAAAAATCACAAGTGTTATAACAGCACAGGTGTGGAC

TACCGGGGGACCGTCAGTGTGACCAAATCAGGGCGCCAGTGCCAGCCATGGAATTCCCAGTATCCCCACACACACACTTT

CACCGCCCTTCGTTTCCCAGAGCTGAATGGAGGCCATTCCTACTGCCGCAACCCAGGGAATCAAAAGGAAGCTCCCTGGT

GCTTCACCTTGGATGAAAACTTTAAGTCTGATCTGTGTGACATCCCAGCGTGCGATTCAAAGGATTCCAAGGAGAAGAAT

AAAATGGAAATCCTGTACATACTAGTGCCAAGTGTGGCCATTCCCCTGGCCATTGCTTTACTCTTCTTCTTCATTTGCGT

CTGTCGGAATAACCAGAAGTCATCGTCGGCACCAGTCCAGAGGCAACCAAAACACGTCAGAGGTCAAAATGTAGAGATGT

CAATGCTGAATGCATATAAACCCAAGAGCAAGGCTAAAGAGCTACCTCTTTCTGCTGTACGCTTTATGGAAGAATTGGGT

GAGTGTGCCTTTGGAAAAATCTATAAAGGCCATCTCTATCTCCCAGGCATGGACCATGCTCAGCTGGTTGCTATCAAGAC

CTTGAAAGACTATAACAACCCCCAGCAATGGACGGAATTTCAACAAGAAGCCTCCCTAATGGCAGAACTGCACCACCCCA

ATATTGTCTGCCTTCTAGGTGCCGTCACTCAGGAACAACCTGTGTGCATGCTTTTTGAGTATATTAATCAGGGGGATCTC

CATGAGTTCCTCATCATGAGATCCCCACACTCTGATGTTGGCTGCAGCAGTGATGAAGATGGGACTGTGAAATCCAGCCT

GGACCACGGAGATTTTCTGCACATTGCAATTCAGATTGCAGCTGGCATGGAATACCTGTCTAGTCACTTCTTTGTCCACA

AGGACCTTGCAGCTCGCAATATTTTAATCGGAGAGCAACTTCATGTAAAGATTTCAGACTTGGGGCTTTCCAGAGAAATT

TACTCCGCTGATTACTACAGGGTCCAGAGTAAGTCCTTGCTGCCCATTCGCTGGATGCCCCCTGAAGCCATCATGTATGG

CAAATTCTCTTCTGATTCAGATATCTGGTCCTTTGGGGTTGTCTTGTGGGAGATTTTCAGTTTTGGACTCCAGCCATATT

ATGGATTCAGTAACCAGGAAGTGATTGAGATGGTGAGAAAACGGCAGCTCTTACCATGCTCTGAAGACTGCCCACCCAGA

ATGTACAGCCTCATGACAGAGTGCTGGAATGAGATTCCTTCTAGGAGACCAAGATTTAAAGATATTCACGTCCGGCTTCG

GTCCTGGGAGGGACTCTCAAGTCACACAAGCTCTACTACTCCTTCAGGGGGAAATGCCACCACACAGACAACCTCCCTCA

GTGCCAGCCCAGTGAGTAATCTCAGTAACCCCAGATATCCTAATTACATGTTCCCGAGCCAGGGTATTACACCACAGGGC

CAGATTGCTGGTTTCATTGGCCCGCCAATACCTCAGAACCAGCGATTCATTCCCATCAATGGATACCCAATACCTCCTGG

ATATGCAGCGTTTCCAGCTGCCCACTACCAGCCAACAGGTCCTCCCAGAGTGATTCAGCACTGCCCACCTCCCAAGAGTC

GGTCCCCAAGCAGTGCCAGTGGGTCGACTAGCACTGGCCATGTGACTAGCTTGCCCTCATCAGGATCCAATCAGGAAGCA

AATATTCCTTTACTACCACACATGTCAATTCCAAATCATCCTGGTGGAATGGGTATCACCGTTTTTGGCAACAAATCTCA

AAAACCCTACAAAATTGACTCAAAGCAAGCATCTTTACTAGGAGACGCCAATATTCATGGACACACCGAATCTATGATTT

CTGCAGAACTGTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

1. J. Biol. Chem. 267:26181 (1992)

2. Oncogene 13:1555 (1996) 

3. Genomics. 41: 283 (1997).

4. Mol Cell Biol. 21: 8329 (2001).

5. Clin Cancer Res. 14: 396 (2008).

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0043-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.