Human CD142/TF/F3/Thromboplastin Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

CD142, also known as TF (tissue factor) and F3 (coagulation factor III), is a single-pass type I transmembrane glycoprotein belonging to the tissue factor family. CD142/TF contains 3 WKS motifs in its extracellular domain (ECD) in addition to a transmembrane (TM) domain and a short cytoplasmic tail.  CD142/TF plays a major role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142/TF acts as a high-affinity receptor for the coagulation factor VII and the [CD142:VII] complex activates factors IX or X by specific limited proteolysis. Platelets and monocytes are known to express CD142/TF under pro-coagulatory and pro-inflammatory stimuli. Unlike the other cofactors, which circulate as nonfunctional precursors, CD142/TF is a potent initiator that is fully functional when expressed on cell surfaces, e.g., on monocytes. CD142/TF also plays a role in HIV-associated coagulopathy.  In addition platelet-dependent monocyte expression of CD142/TF is associated with COVID-19 (Coronavirus Disease 2019)  severity and mortality.

Gene Symbol: CD142; TF; F3; TFA; thromboplastin

NCBI Gene ID: 2152

Uniprot Entry: P13726

Construct Details: The recombinant human CD142-Fc fusion is expressed as a 447 amino acid protein consisting of Ser33 - Glu251 region of CD142/TF and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Source: Human cells stably expressing CD142-Fc protein and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT

        70         80         90        100        110        120
DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG

       130        140        150        160        170        180
TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD

       190        200        210        220        230        240
KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GQEKGEFRES TGTHTCPPCP APELLGGPSV

       250        260        270        280        290        300
FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY

       310        320        330        340        350        360
RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK

       370        380        390        400        410        420
NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG

       430        440
NVFSCSVMHE ALHNHYTQKS LSLSPGK

M.W.: Calculated molecular mass 50.4 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa

Calculated PI: 6.07

Calculated extinction coefficients (M-1 cm-1, at 280nm): 74425

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.