Human CDCP1/CD318/TRASK/SIMA135 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

CDC1P1 (CUB domain-containing protein 1) or CD318 is an 836 amino acid (aa) single pass type I transmembrane protein. CDCP1 contains an N-terminal signal peptide, a 638-aa extracellular domain (ECD) with 3 CUB domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 148-aa cytoplasmic tail. DCP1 is highly expressed in mitotic cells with low expression during interphase. It is expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CDCP1 is up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. CDCP1 interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1.  CDCP1 acts as a substrate for Src family kinases such as SRC and YES as well as by the protein kinase C gamma/PRKCG.  CDCP1 may be involved in cell adhesion and cell matrix association. CDCP1 may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.  CDCP1 belongs to the tetraspanin web involved in the tyrosine phosphorylation-dependent regulation of tumor invasion and metastasis. CDCP1 may be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.  A soluble form of CDCP1 can be produced by proteolytic cleavage at an unidentified site on its N-terminal side and converted to p80 by plasmin, a trypsin-like protease.

Gene Symbol: CDCP1; CD318; TRASK; SIMA135; gp140

NCBI Gene ID: 64866

Uniprot Entry: Q9H5V8

Construct Details: The recombinant human CDCP1-Fc is expressed as an 867 amino acid protein consisting of Phe30 - Val668 region of CDCP1 and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

Source: Human cells stably expressing CDCP1-Fc and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
FEIALPRESN ITVLIKLGTP TLLAKPCYIV ISKRHITMLS IKSGERIVFT FSCQSPENHF

        70         80         90        100        110        120
VIEIQKNIDC MSGPCPFGEV QLQPSTSLLP TLNRTFIWDV KAHKSIGLEL QFSIPRLRQI

       130        140        150        160        170        180
GPGESCPDGV THSISGRIDA TVVRIGTFCS NGTVSRIKMQ EGVKMALHLP WFHPRNVSGF

       190        200        210        220        230        240
SIANRSSIKR LCIIESVFEG EGSATLMSAN YPEGFPEDEL MTWQFVVPAH LRASVSFLNF

       250        260        270        280        290        300
NLSNCERKEE RVEYYIPGST TNPEVFKLED KQPGNMAGNF NLSLQGCDQD AQSPGILRLQ

       310        320        330        340        350        360
FQVLVQHPQN ESNKIYVVDL SNERAMSLTI EPRPVKQSRK FVPGCFVCLE SRTCSSNLTL

       370        380        390        400        410        420
TSGSKHKISF LCDDLTRLWM NVEKTISCTD HRYCQRKSYS LQVPSDILHL PVELHDFSWK

       430        440        450        460        470        480
LLVPKDRLSL VLVPAQKLQQ HTHEKPCNTS FSYLVASAIP SQDLYFGSFC PGGSIKQIQV

       490        500        510        520        530        540
KQNISVTLRT FAPSFQQEAS RQGLTVSFIP YFKEEGVFTV TPDTKSKVYL RTPNWDRGLP

       550        560        570        580        590        600
SLTSVSWNIS VPRDQVACLT FFKERSGVVC QTGRAFMIIQ EQRTRAEEIF SLDEDVLPKP

       610        620        630        640        650        660
SFHHHSFWVN ISNCSPTSGK QLDLLFSVTL TPRTVDLTVS TGTHTCPPCP APELLGGPSV

       670        680        690        700        710        720
FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY

       730        740        750        760        770        780
RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK

       790        800        810        820        830        840
NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG

       850        860
NVFSCSVMHE ALHNHYTQKS LSLSPGK

M.W.: Calculated molecular mass 97.5 kDa; estimated by SDS-PAGE under reducing condition: ~98 kDa and 80-85 kDa two major protein bands and 60-65 kDa one minor band (probably due to proteolytic cleavage in CDCP1 ECD)

Calculated PI: 8.34

Calculated extinction coefficients (M-1 cm-1, at 280nm):  97425

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

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