Human NCR2/CD336/NKp44/LY95 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

NCR2, also known as LY95, NKp44 and CD336, is a single-pass type I transmembrane glycoprotein that contains an extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  NCR2/NKp44/CD336 together with NCR1/NKp36/CD335 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs).  All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells.  Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously.  NCR2 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

 

 

Gene Symbol: NCR2; LY95; CD336; NKP44; NK-p44; dJ149M18.1; NCTR2

 

NCBI Gene ID: 9436

 

Uniprot Entry: O95944

 

Construct Details: Full length human NCR2/CD336/NKp44 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 831 (bp)

 

Gene Insert Sequence:  

ATGGCCTGGCGAGCCCTACACCCACTGCTACTGCTGCTGCTGCTGTTCCCAGGCTCTCAGGCACAATCCA

AGGCTCAGGTACTTCAAAGTGTGGCAGGGCAGACGCTAACCGTGAGATGCCAGTACCCGCCCACGGGCAG

TCTCTACGAGAAGAAAGGCTGGTGTAAGGAGGCTTCAGCACTTGTGTGCATCAGGTTAGTCACCAGCTCC

AAGCCCAGGACGATGGCTTGGACCTCTCGATTCACAATCTGGGACGACCCTGATGCTGGCTTCTTCACTG

TCACCATGACTGATCTGAGAGAGGAAGACTCAGGACATTACTGGTGTAGAATCTACCGCCCTTCTGACAA

CTCTGTCTCTAAGTCCGTCAGATTCTATCTGGTGGTATCTCCAGCCTCTGCCTCCACACAGACCTCCTGG

ACTCCCCGCGACCTGGTCTCTTCACAGACCCAGACCCAGAGCTGTGTGCCTCCCACTGCAGGAGCCAGAC

AAGCCCCTGAGTCTCCATCTACCATCCCTGTCCCTTCACAGCCACAGAACTCCACGCTCCGCCCTGGCCC

TGCAGCCCCCATTGCCCTGGTGCCTGTGTTCTGTGGACTCCTCGTAGCCAAGAGCCTGGTGCTGTCAGCC

CTGCTCGTCTGGTGGGGGGACATATGGTGGAAAACCATGATGGAGCTCAGGAGCCTGGATACCCAAAAAG

CCACCTGCCACCTTCAACAGGTCACGGACCTTCCCTGGACCTCAGTTTCCTCACCTGTAGAGAGAGAAAT

ATTATATCACACTGTTGCAAGGACTAAGATAAGCGATGATGATGATGAACACACTTTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. J. Exp. Med. 188:953 (1997) 

2. Nature 409:1055 (2001)

3.Blood 103:664 (2004)

4. J. Immunol. 172:1455 (2004)

5. Blood. 105: 251 (2005)

 

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.