Human IL1RAPL2/IL1R9/TIGIRR1 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

IL1RAPL1 (interleukin-1 receptor accessory protein-like 1), also known as IL1R9 and TIGIRR1, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin-1 receptor (IL1R) family. Like other family members, IL1RAPL2 contain 3 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL1RAPL2 is most closely related to IL1RAPL1, which together are located at a region on chromosome X associated with X-linked non-syndromic mental retardation.

 

 

Gene Symbol: IL1RAPL2; IL1R9; TIGIRR1; IL-1R9; TIGIRR-1; IL1RAPL-2

 

NCBI Gene ID: 11141

 

Uniprot Entry: Q9NZN1

 

Construct Details: Full length human IL1RAPL2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2061 (bp)

 

Gene Insert Sequence:  

ATGAAGCCACCATTTCTTTTGGCCCTTGTGGTCTGTTCTGTAGTCAGCACAAATCTGAAGATGGTGTCAA

AGAGAAATTCTGTGGATGGCTGCATTGACTGGTCAGTGGATCTCAAGACATACATGGCTTTGGCAGGTGA

ACCAGTCCGAGTGAAATGTGCCCTTTTCTACAGTTATATTCGTACCAACTATAGCACGGCCCAGAGCACT

GGGCTCAGGCTTATGTGGTACAAAAACAAAGGTGATTTGGAAGAGCCCATCATCTTTTCAGAGGTCAGGA

TGAGCAAAGAGGAAGATTCAATATGGTTTCACTCAGCTGAGGCACAAGACAGTGGATTCTACACTTGTGT

TTTAAGAAACTCAACATATTGCATGAAGGTGTCAATGTCCTTGACTGTTGCAGAGAATGAATCAGGCCTG

TGCTACAACAGCAGGATCCGCTATTTAGAAAAATCTGAAGTCACTAAAAGAAAGGAGATCTCCTGTCCAG

ACATGGATGACTTTAAAAAGTCCGATCAGGAGCCTGATGTTGTGTGGTATAAGGAATGCAAGCCAAAAAT

GTGGAGAAGCATAATAATACAGAAAGGAAATGCTCTTCTGATCCAAGAAGTTCAAGAAGAAGATGGAGGA

AATTACACATGTGAACTTAAATATGAAGGAAAACTTGTAAGACGAACAACTGAATTGAAAGTTACAGCTT

TACTCACAGACAAGCCTCCCAAGCCATTGTTCCCCATGGAGAATCAGCCAAGTGTTATAGATGTCCAGCT

GGGTAAGCCTCTGAACATCCCCTGCAAAGCATTCTTCGGATTCAGTGGAGAGTCTGGGCCAATGATCTAC

TGGATGAAAGGAGAAAAGTTTATTGAAGAACTGGCAGGTCACATTAGAGAAGGTGAAATAAGGCTTCTCA

AAGAGCATCTTGGAGAAAAAGAAGTTGAATTGGCACTCATCTTTGACTCAGTTGTGGAAGCTGACCTGGC

GAATTATACCTGCCATGTTGAAAACCGAAATGGACGGAAACATGCCAGTGTTTTGCTGCGTAAAAAGGAT

TTAATCTATAAAATTGAGCTTGCAGGGGGCCTGGGAGCAATCTTCCTCCTCCTTGTACTGCTGGTGGTCA

TTTACAAATGCTACAACATTGAATTGATGCTCTTCTACAGGCAGCACTTTGGAGCTGATGAAACTAATGA

TGACAACAAGGAATATGATGCCTATCTCTCTTACACAAAAGTGGACCAAGATACTTTAGACTGTGACAAT

CCTGAAGAAGAGCAGTTTGCTCTTGAAGTACTGCCAGATGTCCTGGAAAAACACTATGGATATAAACTCT

TCATCCCAGAAAGAGACCTGATTCCAAGTGGAACATACATGGAAGATCTCACAAGATATGTTGAACAAAG

CAGAAGACTTATTATCGTGCTAACTCCAGACTATATTCTCAGACGGGGATGGAGTATTTTCGAACTGGAA

AGCAGACTCCATAACATGCTAGTCAGTGGAGAAATCAAAGTGATTTTGATTGAGTGTACAGAATTAAAAG

GGAAAGTGAATTGCCAGGAAGTGGAATCACTAAAGCGTAGCATCAAACTTCTGTCCCTGATCAAGTGGAA

GGGATCCAAAAGCAGCAAATTAAATTCTAAGTTTTGGAAGCACTTAGTATATGAAATGCCCATCAAGAAA

AAAGAAATGCTACCTCGGTGCCATGTTCTGGACTCCGCAGAACAAGGACTTTTTGGAGAACTCCAGCCTA

TACCCTCTATTGCCATGACCAGTACTTCAGCCACTCTGGTGTCATCTCAGGCTGATCTCCCTGAATTCCA

CCCTTCAGATTCAATGCAAATCAGGCACTGTTGCAGAGGTTATAAACATGAGATACCAGCCACGACCTTG

CCAGTACCTTCCTTAGGCAACCACCATACTTATTGTAACCTGCCTCTGACGCTACTCAACGGACAGCTAC

CCCTTAATAACACCCTGAAAGATACCCAGGAATTTCACAGGAACAGTTCTTTGCTGCCTTTATCCTCCAA

AGAGCTTAGCTTTACCAGTGATATTTGGTAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.