Mouse MSLN/Mesothelin Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Mesothelin (MSLN) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum.  It is overexpressed in mesotheliomas, ovarian and pancreatic cancers as well as in specific squamous cell carcinomas. The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte-potentiating factor (MPF) and MSLN. MPF is secreted and functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. The cleaved form of MSLN remains GPI-anchored on cell-surface and may function as a cell adhesion molecule. Alternative splicing results in multiple transcript variants. Two variant forms exist; variant 1 has an eight amino acid insertion and is rarely expressed, while variant 2 is a truncated form found in cancers but rarely detected in normal individuals. MSLN interacts with CA125/MUC16 and potentiates megakaryocyte colony formation in vitro. MSLN is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment. 

 

 

Gene Symbol: MSLN; MPF; SMRP; CAK1

 

NCBI Gene ID: 56047

 

Uniprot Entry: Q61468

 

Construct Details: Full length mouse MSLN gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1878 (bp)

 

Gene Insert Sequence:  

ATGGCCTTGCCAACAGCTCGACCCCTGCTGGGGTCCTGTGGAAGTCCCATCTGCAGCCGAAGCTTCCTAC

TGCTTCTCCTTAGTCTTGGGTGGATACCACGTCTGCAGACCCAGACTACAAAGACAAGCCAGGAGGCCAC

ACTCCTCCATGCTGTGAACGGTGCCGCTGACTTTGCCAGTCTCCCCACAGGCCTCTTTCTTGGCCTCACA

TGTGAGGAGGTATCTGACCTGAGCATGGAACAAGCCAAGGGGCTGGCTATGGCTGTAAGACAGAAGAACA

TTACACTCCGGGGACATCAGCTGCGTTGTCTGGCACGTCGCCTTCCTAGGCACCTCACCGACGAGGAACT

GAATGCTCTTCCACTGGACCTGCTGCTCTTCCTCAACCCAGCCATGTTTCCAGGGCAACAGGCTTGTGCC

CACTTCTTCTCCCTCATCTCTAAAGCCAATGTGGATGTACTCCCACGGAGGTCTCTGGAGCGCCAGAGGC

TGCTGATGGAGGCTCTGAAGTGCCAGGGCGTGTATGGATTTCAAGTGAGTGAGGCAGATGTGCGGGCTCT

CGGAGGCCTGGCCTGTGACCTGCCTGGGAAATTTGTGGCCAGATCTTCCGAAGTTCTCCTCCCCTGGCTG

GCAGGATGCCAAGGACCCCTGGACCAGAGCCAGGAAAAGGCAGTCAGGGAGGTTCTGAGGAGTGGAAGAA

CCCAATATGGCCCCCCATCGAAGTGGTCAGTCTCCACCCTGGATGCCCTGCAGAGCTTGGTAGCAGTGTT

GGATGAGTCCATCGTCCAGAGCATCCCCAAGGATGTCAAAGCTGAATGGCTGCAACACATCTCCAGAGAC

CCCTCCAGGCTGGGGTCTAAGCTGACCGTCATACACCCAAGGTTCCGACGGGATGCAGAACAGAAAGCCT

GCCCTCCAGGGAAGGAGCCCTACAAGGTGGATGAAGACCTCATCTTCTACCAGAATTGGGAGCTGGAGGC

TTGTGTAGATGGCACCATGCTGGCCAGACAAATGGACCTTGTGAACGAGATTCCCTTCACCTATGAGCAG

CTCAGTATCTTTAAGCACAAACTGGACAAGACCTACCCACAAGGCTATCCTGAGTCCCTGATCCAGCAGC

TGGGTCACTTCTTCAGATATGTTAGCCCTGAAGACATCCACCAGTGGAATGTGACCTCACCAGACACAGT

GAAAACTCTGCTCAAAGTCAGCAAAGGACAAAAGATGAATGCTCAGGCGATTGCCTTGGTCGCCTGCTAT

CTTCGGGGAGGAGGCCAGCTGGACGAGGATATGGTAAAAGCCCTGGGCGACATCCCGTTAAGCTATCTAT

GTGACTTCAGCCCCCAGGATCTGCACTCGGTACCCTCCAGTGTCATGTGGCTGGTTGGGCCCCAAGACCT

GGACAAGTGCAGCCAGAGGCATCTGGGTCTCCTCTACCAGAAGGCCTGCTCAGCCTTCCAGAATGTGAGC

GGCCTAGAATACTTTGAGAAAATCAAGACATTCCTGGGTGGGGCCTCCGTGAAGGACCTGCGGGCCCTCA

GCCAGCACAATGTGAGCATGGACATAGCCACTTTCAAGAGGCTGCAGGTGGATTCCCTGGTGGGGCTGAG

TGTGGCTGAGGTACAGAAACTTCTGGGGCCAAACATTGTGGACCTGAAGACCGAGGAGGATAAAAGCCCT

GTCCGTGACTGGCTGTTCCGGCAGCATCAGAAAGACCTAGACAGGCTGGGTTTGGGACTTCAGGGTGGCA

TCCCCAATGGCTACCTGGTCCTGGACTTCAATGTCCGAGAGGCCTTCTCCAGCAGAGCCTCACTCCTTGG

GCCAGGATTTGTATTAATATGGATTCCAGCTCTGCTCCCAGCTTTAAGGCTGAGCTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) Reverse (or 3’-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. J. Biol. Chem. 269:805-808 (1994)

2. J. Biol. Chem. 270:21984-21990 (1995)

3. Clin. Cancer Res. 12:4225-4231 (2006)

4. Proc. Natl. Acad. Sci. 96:11531-11536 (1999)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.