Human INSRR/IRR Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

INSRR (insulin receptor-related protein) receptor tyrosine kinase (RTK), is a single-pass type I transmembrane glycoprotein that belongs to the insulin receptor (InsR) subfamily of the tyrosine protein kinase family.  INSRR is expressed as a 1297-aa precursor and cleavage of the precursor generates alpha and beta subunits. The alpha subunit comprises a 716-aa extracellular domain (ECD) while the beta subunit contains a 175-aa extracellular domain (ECD), a transmembrane region (TM), and a 354-aa intracellular kinase domain. IINSRR probably exist as a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain for signaling. INSRR may function as a pH sensing receptor which is activated by increased extracellular pH. INSRR activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.

 

 

Gene Symbol: INSRR; IRR

 

NCBI Gene ID: 3645

 

Uniprot Entry: P14616

 

Construct Details: Full length human INSRR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3894 (bp)

 

Gene Insert Sequence:  

ATGGCAGTGCCTAGTCTGTGGCCCTGGGGAGCATGCCTGCCTGTGATCTTCCTCTCCTTGGGATTTGGCC

TGGATACAGTAGAGGTGTGCCCCAGCCTGGATATTCGCTCAGAGGTGGCAGAGCTTCGTCAGCTGGAGAA

CTGCAGCGTGGTGGAGGGCCACCTGCAGATCCTGCTCATGTTCACAGCCACCGGGGAGGACTTCCGCGGC

CTCAGCTTCCCTCGCCTCACCCAGGTCACCGACTACCTGCTGCTCTTCCGTGTCTACGGACTGGAGAGCC

TGCGCGACCTCTTCCCCAACCTAGCAGTCATCCGCGGGACGCGCCTCTTCCTGGGCTATGCACTGGTCAT

CTTTGAGATGCCACATCTGCGTGACGTGGCACTGCCTGCACTTGGGGCCGTGCTGCGTGGGGCTGTGCGT

GTGGAGAAGAACCAGGAGCTCTGCCACCTCTCCACCATTGACTGGGGACTGCTGCAGCCAGCACCTGGCG

CCAACCACATCGTGGGCAACAAGCTGGGCGAGGAGTGTGCTGACGTGTGCCCTGGTGTGCTGGGTGCTGC

TGGTGAGCCCTGTGCCAAGACCACCTTCAGCGGGCACACTGACTACAGATGCTGGACCTCCAGCCACTGC

CAGAGAGTGTGCCCCTGCCCCCATGGGATGGCTTGCACAGCGAGGGGCGAGTGCTGCCACACCGAATGCC

TGGGGGGCTGCAGCCAGCCAGAAGACCCTCGTGCCTGTGTAGCTTGCCGCCACCTCTACTTCCAGGGTGC

CTGCCTGTGGGCCTGCCCGCCAGGCACCTACCAGTATGAGTCCTGGCGCTGTGTCACAGCTGAGCGCTGT

GCCAGCCTGCACTCTGTGCCCGGCCGTGCCTCCACCTTCGGCATACACCAGGGCAGTTGCCTGGCCCAGT

GCCCTTCTGGCTTCACCCGTAATAGCAGCAGCATATTCTGCCACAAGTGCGAGGGGCTGTGCCCTAAAGA

GTGCAAGGTAGGCACCAAGACCATCGACTCCATCCAGGCGGCACAGGATCTTGTGGGCTGCACGCATGTG

GAGGGAAGCCTCATCCTCAACCTTCGCCAGGGCTACAACCTGGAGCCACAGCTGCAGCACAGCCTGGGGC

TGGTAGAAACCATTACTGGCTTCCTCAAAATCAAGCACTCCTTTGCCCTCGTGTCCCTGGGCTTTTTCAA

GAACCTCAAACTAATCCGGGGAGACGCCATGGTGGATGGGAACTACACTCTCTACGTGCTGGACAACCAG

AACCTACAACAGCTAGGGTCCTGGGTGGCCGCGGGGCTCACCATTCCCGTGGGCAAGATCTACTTCGCCT

TCAACCCGCGCCTCTGCTTGGAACACATCTACCGACTGGAGGAGGTGACAGGCACGCGAGGTCGGCAGAA

CAAGGCTGAGATCAACCCCCGCACCAACGGAGACCGCGCCGCCTGCCAGACTCGCACCCTGCGCTTCGTG

TCCAACGTGACGGAGGCAGACCGCATCCTGCTACGCTGGGAGCGCTATGAGCCACTGGAGGCCCGCGACC

TGCTCAGCTTCATCGTGTACTACAAGGAGTCCCCATTCCAGAACGCCACAGAGCACGTGGGTCCAGATGC

TTGTGGAACCCAGAGCTGGAACCTGCTGGATGTGGAGCTGCCCCTAAGCCGCACCCAGGAGCCAGGGGTG

ACCCTAGCCTCCCTCAAGCCTTGGACACAGTACGCAGTGTTTGTGCGGGCCATCACGCTAACCACTGAGG

AGGACAGCCCTCATCAAGGAGCCCAGAGTCCCATCGTCTACCTCCGAACGCTGCCTGCAGCTCCCACGGT

GCCCCAAGACGTCATCTCCACGTCCAACTCCTCCTCCCACCTCCTGGTGCGCTGGAAGCCACCGACCCAG

CGCAATGGGAACCTCACCTACTACCTGGTGCTGTGGCAGCGGCTGGCAGAGGACGGCGACCTCTACCTCA

ATGACTACTGCCACCGCGGCTTGCGGCTGCCCACCAGCAACAACGATCCGCGCTTCGACGGCGAAGACGG

GGATCCTGAGGCCGAGATGGAGTCCGACTGCTGCCCTTGCCAGCACCCACCTCCTGGTCAGGTTCTGCCC

CCGCTGGAGGCGCAAGAGGCCTCGTTCCAGAAGAAGTTTGAAAACTTTCTACACAACGCGATCACCATCC

CCATATCCCCTTGGAAGGTGACGTCCATCAACAAGAGCCCCCAAAGGGACTCAGGGCGGCACCGCCGGGC

AGCTGGGCCCCTCCGGCTGGGGGGCAACAGCTCGGATTTCGAGATCCAGGAGGACAAGGTGCCCCGTGAG

CGAGCGGTGCTGAGCGGCCTGCGCCACTTCACGGAATACCGGATCGACATCCATGCCTGCAACCACGCGG

CGCACACCGTGGGCTGCAGCGCCGCCACCTTCGTCTTTGCGCGCACCATGCCCCACAGAGAGGCTGATGG

TATTCCAGGAAAGGTGGCCTGGGAGGCCTCCAGCAAGAACAGTGTCCTTCTGCGCTGGCTCGAGCCACCA

GACCCCAACGGACTCATCCTCAAGTACGAAATCAAGTACCGCCGCTTGGGAGAGGAGGCCACAGTGCTGT

GTGTGTCCCGTCTTCGATATGCGAAGTTTGGGGGAGTCCACCTGGCCCTGCTGCCCCCTGGAAACTACTC

TGCCAGGGTTAGGGCAACCTCACTGGCTGGCAATGGCTCTTGGACAGACAGTGTTGCCTTCTACATCCTT

GGCCCAGAGGAGGAGGATGCTGGGGGGCTGCATGTCCTCCTCACTGCCACCCCTGTGGGGCTCACGCTGC

TCATCGTTCTTGCTGCCCTTGGTTTCTTCTACGGCAAGAAGAGAAACAGAACCCTGTATGCTTCTGTGAA

TCCAGAGTACTTCAGCGCCTCTGATATGTATGTCCCTGATGAATGGGAGGTGCCTCGGGAGCAGATCTCG

ATAATCCGGGAACTGGGCCAGGGCTCTTTTGGGATGGTATATGAGGGGCTGGCACGAGGACTTGAGGCTG

GAGAGGAGTCCACACCCGTGGCCCTGAAGACGGTGAATGAGCTGGCCAGCCCACGGGAATGCATTGAGTT

CCTCAAGGAAGCTTCTGTCATGAAAGCCTTCAAGTGTCACCATGTGGTGCGTCTCCTGGGTGTGGTATCT

CAGGGCCAGCCAACTCTGGTCATCATGGAGTTAATGACCCGTGGGGACCTCAAGAGCCATCTTCGATCTT

TGCGGCCTGAGGCAGAGAACAACCCTGGGCTCCCACAGCCAGCATTGGGGGAAATGATCCAAATGGCTGG

TGAGATTGCAGACGGCATGGCCTACCTTGCTGCCAACAAGTTTGTGCACCGAGATCTAGCAGCCCGCAAC

TGCATGGTGTCCCAGGACTTCACCGTCAAGATCGGGGACTTCGGGATGACTCGGGACGTGTATGAGACAG

ACTATTACCGCAAGGGTGGGAAGGGGCTGCTGCCCGTGCGCTGGATGGCCCCCGAGTCCCTCAAAGATGG

GATCTTCACCACCCACTCGGATGTCTGGTCCTTTGGCGTGGTACTCTGGGAGATTGTGACCCTGGCAGAA

CAACCCTACCAGGGCCTGTCCAATGAGCAGGTGCTGAAGTTCGTCATGGATGGCGGGGTCCTGGAGGAGC

TGGAGGGCTGTCCCCTTCAGCTGCAGGAGCTGATGAGCCGCTGCTGGCAGCCGAACCCACGCCTGCGCCC

ATCTTTCACACACATTCTGGACAGCATACAGGAGGAGCTGCGGCCCTCCTTCCGCCTCCTCTCCTTCTAC

TACAGCCCGGAATGCCGGGGGGCCCGGGGCTCCCTGCCTACCACCGATGCAGAGCCTGACTCCTCACCCA

CTCCAAGAGACTGCAGCCCTCAAAATGGGGGTCCAGGGCACTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.