Human LTK/TYK1 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

LTK receptor yrosine-protein kinase (RTK), is a single-pass type I transmembrane glycoprotein that belongs to the insulin receptor (InsR) subfamily of the tyrosine protein kinase family. LTK comprises a 408-aa large extracellular domain (ECD), a transmembrane region (TM), and a 415-aa intracellular kinase domain.  LTK is expressed in non-hematopoietic cell lines and T- and B-cell lines.  The exact function of LTK is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. LTK may be involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport.

 

 

Gene Symbol: LTK; TYK1

 

NCBI Gene ID: 4058

 

Uniprot Entry: P29376

 

Construct Details: Full length human LTK gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2595 (bp)

 

Gene Insert Sequence:  

ATGGGCTGCTGGGGACAGCTGCTGGTGTGGTTCGGAGCCGCGGGCGCCATTCTCTGCTCTAGCCCGGGGT

CCCAGGAGACTTTTCTGCGGTCCTCGCCCCTGCCGCTGGCAAGTCCCAGCCCCCGGGACCCGAAAGTCAG

CGCCCCGCCTAGTATCTTGGAGCCAGCCTCCCCGCTGAATTCTCCGGGCACCGAGGGGTCTTGGCTGTTT

TCTACCTGCGGGGCCAGCGGCCGGCATGGGCCCACACAGACACAATGTGACGGGGCGTACGCGGGGACCA

GCGTGGTGGTGACCGTGGGGGCCGCCGGGCAGCTGAGAGGCGTGCAGCTGTGGCGCGTGCCGGGCCCTGG

CCAGTATCTGATCTCAGCCTACGGAGCCGCGGGCGGCAAAGGCGCCAAGAACCACCTGTCGCGGGCGCAT

GGCGTCTTCGTCTCAGCAATCTTCTCCCTCGGTCTCGGGGAGTCGCTGTACATCCTGGTGGGGCAGCAGG

GAGAGGACGCCTGTCCCGGAGGTAGCCCGGAGAGCCAGCTCGTCTGCCTCGGGGAGTCTCGAGCCGTTGA

AGAGCACGCGGCGATGGATGGGAGCGAAGGGGTCCCGGGGTCGCGGCGCTGGGCGGGAGGTGGCGGGGGT

GGCGGGGGCGCCACCTACGTTTTCCGGGTGCGCGCTGGCGAGCTGGAACCGTTGCTGGTGGCGGCCGGAG

GCGGCGGTCGGGCCTACCTGAGGCCGCGGGACCGAGGCCGGACTCAGGCCTCCCCCGAGAAACTGGAGAA

CCGCTCGGAGGCGCCCGGGAGCGGCGGGAGAGGCGGGGCGGCAGGTGGTGGGGGCGGCTGGACGTCGCGG

GCTCCCTCTCCGCAGGCCGGCCGCTCACTGCAGGAGGGGGCGGAGGGCGGCCAGGGCTGCTCCGAGGCTT

GGGCGACCCTTGGCTGGGCCGCGGCCGGCGGCTTCGGGGGCGGCGGCGGGGCCTGCACTGCGGGCGGAGG

CGGCGGCGGCTACAGGGGGGGCGACGCTTCAGAGACTGACAACCTCTGGGCTGATGGGGAAGATGGAGTA

TCCTTCATACACCCCAGCAGCGAGCTCTTCCTGCAGCCTCTGGCAGTCACCGAGAACCACGGAGAGGTAG

AGATCCGAAGGCACCTCAACTGCAGTCACTGCCCTTTGAGAGACTGCCAATGGCAGGCAGAGCTCCAGCT

GGCTGAATGCCTGTGCCCAGAAGGCATGGAGCTAGCTGTGGATAACGTCACCTGCATGGACCTGCACAAG

CCCCCAGGCCCTCTGGTTCTGATGGTGGCTGTGGTGGCAACCTCAACACTGAGCCTCCTTATGGTGTGTG

GGGTCCTGATTCTGGTGAAGCAGAAGAAGTGGCAGGGCCTGCAGGAGATGAGGCTGCCGAGCCCTGAGCT

TGAGCTGAGCAAGCTTCGAACCTCTGCCATCAGGACAGCCCCCAATCCCTATTATTGCCAGGTGGGGCTT

GGCCCGGCCCAGTCCTGGCCTCTGCCACCAGGTGTCACCGAGGTTTCCCCAGCCAATGTTACTCTGCTCA

GAGCCCTGGGCCATGGTGCCTTTGGGGAGGTGTATGAGGGACTGGTAATTGGCCTTCCTGGGGACTCCAG

TCCCCTGCAGGTAGCTATCAAGACCCTGCCAGAACTCTGCTCGCCTCAGGATGAGCTGGATTTCCTCATG

GAGGCCCTCATCATCAGCAAGTTTCGCCATCAGAACATTGTGCGGTGTGTGGGGCTCAGCCTCAGGGCCA

CCCCTCGCCTCATTCTGCTGGAACTGATGTCTGGAGGGGACATGAAGAGTTTCCTGAGGCACAGTCGGCC

ACACCTGGGCCAGCCATCACCTCTGGTCATGCGGGACCTGCTGCAACTGGCCCAGGACATAGCCCAGGGC

TGCCACTACCTGGAGGAAAATCACTTCATCCACAGGGATATTGCCGCCCGGAACTGCCTGCTGAGCTGCG

CTGGACCCAGCCGAGTGGCCAAGATTGGGGACTTTGGGATGGCACGAGATATCTACCGGGCCAGTTATTA

CCGCAGGGGGGACCGGGCCTTGCTCCCAGTCAAGTGGATGCCCCCAGAGGCCTTCCTGGAGGGCATCTTC

ACATCCAAGACAGATTCCTGGTCTTTTGGGGTGCTGCTCTGGGAGATCTTCTCACTGGGCTACATGCCCT

ATCCTGGGCGCACCAACCAGGAGGTGCTGGACTTCGTCGTTGGAGGAGGCCGGATGGACCCTCCTAGGGG

CTGCCCAGGGCCTGTGTACCGCATCATGACCCAGTGTTGGCAGCACGAGCCTGAGCTCCGCCCTAGCTTT

GCCAGCATCTTGGAGCGTCTGCAGTACTGCACTCAGGACCCGGATGTGCTGAATTCACTCCTGCCAATGG

AGCTGGGGCCCACCCCAGAGGAGGAAGGGACTTCTGGGCTGGGGAACAGATCTTTGGAGTGCCTAAGACC

CCCACAGCCCCAGGAACTGAGTCCAGAGAAGTTGAAAAGCTGGGGAGGTAGCCCTCTTGGCCCCTGGCTG

TCCTCTGGCCTCAAGCCCCTCAAATCCAGGGGCCTCCAACCTCAGAACCTTTGGAATCCCACTTATCGCT

CCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.