Human TLR3/CD283 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA
TLR3 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucinerich repeats (LRRs) and a cytoplasmic tail with a Toll/IL1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR3 contains 22 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes double-stranded RNA (dsRNA) associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. TLR3 may thus play a role in host defense against viruses.
Gene Symbol: TLR3; CD283; IIAE2
NCBI Gene ID: 7098
Uniprot Entry: O60603
Construct Details: Full length human TLR3/CD283 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter +/- an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.
Vector Type: pLTC or pLTC-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- selection marker, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Sequence:
ATGAGACAGACTTTGCCTTGTATCTACTTTTGGGGGGGCCTTTTGCCCTTTGGGATGCTGTGTGCATCCT
CCACCACCAAGTGCACTGTTAGCCATGAAGTTGCTGACTGCAGCCACCTGAAGTTGACTCAGGTACCCGA
TGATCTACCCACAAACATAACAGTGTTGAACCTTACCCATAATCAACTCAGAAGATTACCAGCCGCCAAC
TTCACAAGGTATAGCCAGCTAACTAGCTTGGATGTAGGATTTAACACCATCTCAAAACTGGAGCCAGAAT
TGTGCCAGAAACTTCCCATGTTAAAAGTTTTGAACCTCCAGCACAATGAGCTATCTCAACTTTCTGATAA
AACCTTTGCCTTCTGCACGAATTTGACTGAACTCCATCTCATGTCCAACTCAATCCAGAAAATTAAAAAT
AATCCCTTTGTCAAGCAGAAGAATTTAATCACATTAGATCTGTCTCATAATGGCTTGTCATCTACAAAAT
TAGGAACTCAGGTTCAGCTGGAAAATCTCCAAGAGCTTCTATTATCAAACAATAAAATTCAAGCGCTAAA
AAGTGAAGAACTGGATATCTTTGCCAATTCATCTTTAAAAAAATTAGAGTTGTCATCGAATCAAATTAAA
GAGTTTTCTCCAGGGTGTTTTCACGCAATTGGAAGATTATTTGGCCTCTTTCTGAACAATGTCCAGCTGG
GTCCCAGCCTTACAGAGAAGCTATGTTTGGAATTAGCAAACACAAGCATTCGGAATCTGTCTCTGAGTAA
CAGCCAGCTGTCCACCACCAGCAATACAACTTTCTTGGGACTAAAGTGGACAAATCTCACTATGCTCGAT
CTTTCCTACAACAACTTAAATGTGGTTGGTAACGATTCCTTTGCTTGGCTTCCACAACTAGAATATTTCT
TCCTAGAGTATAATAATATACAGCATTTGTTTTCTCACTCTTTGCACGGGCTTTTCAATGTGAGGTACCT
GAATTTGAAACGGTCTTTTACTAAACAAAGTATTTCCCTTGCCTCACTCCCCAAGATTGATGATTTTTCT
TTTCAGTGGCTAAAATGTTTGGAGCACCTTAACATGGAAGATAATGATATTCCAGGCATAAAAAGCAATA
TGTTCACAGGATTGATAAACCTGAAATACTTAAGTCTATCCAACTCCTTTACAAGTTTGCGAACTTTGAC
AAATGAAACATTTGTATCACTTGCTCATTCTCCCTTACACATACTCAACCTAACCAAGAATAAAATCTCA
AAAATAGAGAGTGATGCTTTCTCTTGGTTGGGCCACCTAGAAGTACTTGACCTGGGCCTTAATGAAATTG
GGCAAGAACTCACAGGCCAGGAATGGAGAGGTCTAGAAAATATTTTCGAAATCTATCTTTCCTACAACAA
GTACCTGCAGCTGACTAGGAACTCCTTTGCCTTGGTCCCAAGCCTTCAACGACTGATGCTCCGAAGGGTG
GCCCTTAAAAATGTGGATAGCTCTCCTTCACCATTCCAGCCTCTTCGTAACTTGACCATTCTGGATCTAA
GCAACAACAACATAGCCAACATAAATGATGACATGTTGGAGGGTCTTGAGAAACTAGAAATTCTCGATTT
GCAGCATAACAACTTAGCACGGCTCTGGAAACACGCAAACCCTGGTGGTCCCATTTATTTCCTAAAGGGT
CTGTCTCACCTCCACATCCTTAACTTGGAGTCCAACGGCTTTGACGAGATCCCAGTTGAGGTCTTCAAGG
ATTTATTTGAACTAAAGATCATCGATTTAGGATTGAATAATTTAAACACACTTCCAGCATCTGTCTTTAA
TAATCAGGTGTCTCTAAAGTCATTGAACCTTCAGAAGAATCTCATAACATCCGTTGAGAAGAAGGTTTTC
GGGCCAGCTTTCAGGAACCTGACTGAGTTAGATATGCGCTTTAATCCCTTTGATTGCACGTGTGAAAGTA
TTGCCTGGTTTGTTAATTGGATTAACGAGACCCATACCAACATCCCTGAGCTGTCAAGCCACTACCTTTG
CAACACTCCACCTCACTATCATGGGTTCCCAGTGAGACTTTTTGATACATCATCTTGCAAAGACAGTGCC
CCCTTTGAACTCTTTTTCATGATCAATACCAGTATCCTGTTGATTTTTATCTTTATTGTACTTCTCATCC
ACTTTGAGGGCTGGAGGATATCTTTTTATTGGAATGTTTCAGTACATCGAGTTCTTGGTTTCAAAGAAAT
AGACAGACAGACAGAACAGTTTGAATATGCAGCATATATAATTCATGCCTATAAAGATAAGGATTGGGTC
TGGGAACATTTCTCTTCAATGGAAAAGGAAGACCAATCTCTCAAATTTTGTCTGGAAGAAAGGGACTTTG
AGGCGGGTGTTTTTGAACTAGAAGCAATTGTTAACAGCATCAAAAGAAGCAGAAAAATTATTTTTGTTAT
AACACACCATCTATTAAAAGACCCATTATGCAAAAGATTCAAGGTACATCATGCAGTTCAACAAGCTATT
GAACAAAATCTGGATTCCATTATATTGGTTTTCCTTGAGGAGATTCCAGATTATAAACTGAACCATGCAC
TCTGTTTGCGAAGAGGAATGTTTAAATCTCACTGCATCTTGAACTGGCCAGTTCAGAAAGAACGGATAGG
TGCCTTTCGTCATAAATTGCAAGTAGCACTTGGATCCAAAAACTCTGTACATTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.