Human SLC34A1/NaPi2a/NaPi3/NPT2 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

SLC34A1 (sodium-dependent phosphate transport protein 2A) or NaPi2a is a 8-pass type II transmembrane protein belonging to the SLC34A transporter family. SLC34A1 acts as a type II sodium-phosphate cotransporter. SLC34A1 is involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. SLC34A1 probably mediates 70-80% of the apical influx.  SLC34A1 interacts via its C-terminal region with PDZK2 and with SLC9A3R1. Mutations in the SLC34A1 gene are associated with hypophosphatemia nephrolithiasis/osteoporosis 1 (NPHLOP1) and hypercalcemia, infantile, 2 (HCINF2).

 

 

Gene Symbol: SLC34A1; NaPi2a; NPT2; FRTS2; SLC11; HCINF2; NaPi-2a; NAPI-3; NPTIIa; NPHLOP1; SLC17A2

 

NCBI Gene ID: 6569

 

Uniprot Entry: Q06495

 

Construct Details: Full length human SLC34A1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1920 (bp)

 

Gene Insert Sequence:  

ATGTTGTCCTACGGAGAGAGGCTGGGGTCCCCTGCTGTCTCCCCACTCCCAGTCCGTGGGGGGCATGTGA

TGCGAGGGACGGCCTTTGCCTACGTGCCCAGCCCTCAGGTCCTACACAGGATCCCGGGGACCTCTGCCTA

TGCCTTCCCCAGCCTGGGCCCTGTGGCCCTTGCTGAGCACACCTGCCCCTGTGGGGAGGTCCTGGAGCGC

CATGAACCACTGCCTGCCAAGCTGGCCCTGGAGGAGGAGCAGAAGCCAGAGTCCAGGCTGGTCCCCAAGC

TGCGCCAGGCTGGCGCCATGCTGCTCAAGGTGCCACTGATGCTCACCTTCCTCTACCTCTTCGTCTGCTC

CCTGGACATGCTCAGCTCGGCCTTCCAGCTGGCTGGAGGGAAGGTGGCTGGTGACATCTTCAAGGATAAC

GCCATCCTGTCCAACCCGGTGGCCGGGCTGGTGGTGGGGATCCTGGTGACCGTGCTGGTGCAGAGCTCCA

GCACCTCCACATCCATCATCGTCAGCATGGTCTCCTCTGGCTTGCTGGAGGTGAGCTCTGCCATCCCCAT

CATCATGGGCTCCAACATCGGCACCTCTGTCACCAACACCATCGTGGCCCTGATGCAGGCGGGGGACAGG

ACTGACTTCCGGCGGGCCTTCGCGGGGGCCACGGTGCATGACTGCTTTAACTGGCTGTCAGTGCTGGTCC

TGCTGCCCCTGGAGGCTGCCACTGGCTACCTGCACCACATCACTCGACTTGTGGTGGCCTCCTTCAACAT

CCATGGTGGCCGTGATGCTCCTGACCTGCTCAAGATCATCACAGAGCCCTTCACGAAGCTCATCATCCAG

CTGGACGAGTCTGTGATAACCAGCATTGCCACTGGTGATGAGTCCCTGAGGAACCACAGTCTCATCCAGA

TCTGGTGCCACCCAGACTCCTTACAGGCTCCCACCTCCATGTCCAGAGCAGAGGCCAACTCCAGCCAGAC

CCTTGGAAATGCCACCATGGAGAAATGCAACCACATCTTTGTGGACACTGGCCTACCGGACCTGGCTGTG

GGGCTCATCCTGCTGGCAGGATCCCTGGTGCTGCTGTGCACCTGCCTCATCCTCCTAGTCAAGATGCTCA

ACTCCCTGCTCAAGGGCCAAGTGGCCAAGGTCATCCAGAAGGTCATCAATACGGACTTCCCTGCCCCCTT

CACCTGGGTCACAGGCTACTTTGCCATGGTGGTGGGCGCCAGCATGACCTTCGTGGTCCAGAGCAGTTCT

GTGTTCACCTCGGCCATCACCCCACTCATCGGTCTTGGTGTGATCAGCATTGAGAGGGCCTACCCGCTCA

CACTGGGTTCCAACATCGGCACCACCACCACGGCCATCCTGGCTGCCCTGGCCAGCCCCAGGGAGAAGCT

GTCCAGCGCTTTCCAGATTGCCCTCTGTCACTTCTTCTTCAACATCTCGGGTATCCTTCTGTGGTACCCG

GTGCCCTGCACACGCCTGCCCATCCGCATGGCCAAGGCGCTGGGGAAACGCACGGCCAAGTACCGCTGGT

TTGCCGTCCTCTATCTCCTTGTCTGCTTCCTGCTGCTGCCCTCACTGGTGTTTGGCATCTCCATGGCAGG

CTGGCAGGTCATGGTAGGTGTGGGCACGCCCTTCGGGGCCCTGCTGGCCTTCGTGGTGCTCATCAATGTC

CTGCAGAGTCGGAGTCCCGGGCACCTGCCCAAGTGGTTACAGACATGGGACTTCCTGCCTCGCTGGATGC

ACTCCCTGAAGCCCCTGGACCACCTCATCACCCGCGCCACCCTATGCTGTGCCAGGCCTGAGCCCCGCTC

ACCCCCGCTGCCCCCCAGGGTCTTCCTGGAGGAGCTACCCCCTGCCACACCCTCCCCCCGTCTTGCACTG

CCTGCTCACCACAATGCCACCCGCCTCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.