Human CLEC12B Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CLEC12B (C-type lectin domain family 12 member A) is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC12B contains 1 C-type lectin domain in the extracellular region and 1 copy of cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitor motif) motif. The ITIM motif is involved in modulation of cellular responses and the phosphorylated ITIM can bind the SH2 domain of several SH2-containing phosphatases. CLEC12B may act as a cell surface receptor that protects target cells against natural killer cell-mediated lysis. CLEC12B may modulate signaling cascades and tyrosine phosphorylation of target MAP kinases.  

 

 

Gene Symbol: CLEC12B

 

NCBI Gene ID: 387837

 

Uniprot Entry: Q2HXU8

 

Construct Details: Full length human CLEC12B gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 831 (bp)

 

Gene Insert Sequence:  

ATGTCTGAAGAAGTGACCTACGCGACACTCACATTTCAGGATTCTGCTGGAGCAAGGAATAACCGAGATG

GAAATAACCTAAGAAAAAGAGGGCATCCAGCTCCATCTCCCATTTGGCGTCATGCTGCTCTGGGTCTGGT

AACTCTTTGCCTGATGTTGCTGATTGGGCTGGTGACATTGGGGATGATGTTTTTGCAGATATCTAATGAC

ATTAACTCAGATTCAGAGAAATTGAGTCAACTTCAGAAAACCATCCAACAGCAGCAGGATAACTTATCCC

AGCAACTGGGCAACTCCAACAACTTGTCCATGGAGGAGGAATTTCTCAAGTCACAGATCTCCAGTGTACT

GAAGAGGCAGGAACAAATGGCCATCAAACTGTGCCAAGAGCTAATCATTCATACTTCAGACCACAGATGT

AATCCATGTCCTAAGATGTGGCAATGGTACCAAAATAGTTGCTACTATTTTACAACAAATGAGGAGAAAA

CCTGGGCTAACAGTAGAAAGGACTGCATAGACAAGAACTCCACCCTAGTGAAGATAGACAGTTTGGAAGA

AAAGGATTTTCTTATGTCACAGCCATTACTCATGTTTTCGTTCTTTTGGCTGGGATTATCATGGGACTCC

TCTGGCAGAAGTTGGTTCTGGGAAGATGGCTCTGTTCCCTCTCCATCCTTATTTAGTACTAAAGAACTTG

ACCAGATCAATGGATCCAAAGGATGTGCTTATTTTCAAAAAGGAAATATTTATATTTCTCGCTGTAGTGC

TGAAATTTTTTGGATTTGCGAGAAGACAGCTGCCCCAGTGAAGACTGAGGATTTGGATTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.