Human IgSF3/EWI3/LCDD Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

IgSF2 or EWI3 is a 1194 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the EWI subfamily of the Immunoglobulin superfamily (IgSF).  IgSF3 contains an N-terminal signal peptide, a 1105-aa extracellular domain (ECD) with 8 Ig-like C2-type domains, an EWI (Glu-Trp-Ile)-motif and 5 potential glycosylation sites, a transmembrane domain (TM), and a 49-aa cytoplasmic tail. IThere is likely an interchromosomal Alu-mediated fusion between IGSF3 on 1p13.1 and GGT on 22q11.2.  A mutation in the IgSF3 gene has been associated with bilateral nasolacrimal duct obstruction (LCDD), a condition resulting in the imbalance between tear production and tear drainage caused by failure of the nasolacrimal duct to open into the inferior meatus.

 

 

Gene Symbol: IgSF3; EWI3; V8; LCDD; EWI-3; IGSF-3

 

NCBI Gene ID: 3321

 

Uniprot Entry: O75054

 

Construct Details: Full length human IgSF3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3585 (bp)

 

Gene Insert Sequence:  

ATGAAGTGCTTTTTCCCGGTGCTGAGCTGTCTGGCTGTGCTGGGTGTGGTGTCAGCACAGCGGCAGGTCA

CCGTTCAGGAAGGACCCTTGTACCGCACGGAGGGCTCCCACATCACTATCTGGTGCAATGTGAGTGGCTA

CCAGGGACCTTCTGAGCAGAATTTCCAGTGGTCCATTTACCTGCCTTCGTCGCCAGAGCGAGAGGTGCAG

ATCGTCAGCACCATGGACTCTTCCTTCCCCTATGCCATCTACACCCAGCGCGTCCGCGGAGGGAAGATCT

TCATAGAAAGAGTCCAGGGGAACTCAACCCTATTGCACATCACAGATCTTCAGGCCCGGGATGCCGGGGA

GTATGAATGCCACACACCCAGCACTGATAAGCAATACTTTGGGAGTTACAGTGCAAAGATGAACCTAGTG

GTGATCCCAGACTCCCTGCAGACCACTGCCATGCCCCAGACTCTGCACAGAGTGGAGCAGGACCCGCTGG

AGCTCACTTGTGAGGTGGCCTCAGAGACCATTCAGCACAGCCACCTGTCTGTGGCCTGGCTCCGGCAGAA

AGTTGGCGAGAAGCCCGTGGAGGTCATCTCCCTGAGCCGAGATTTCATGCTTCACTCCAGCAGCGAATAT

GCCCAGAGGCAGAGCCTGGGGGAGGTGCGGCTGGACAAGCTGGGGAGGACCACCTTCCGCCTCACCATCT

TCCACCTGCAGCCTTCTGACCAGGGCGAATTCTACTGCGAGGCCGCCGAGTGGATCCAGGATCCGGATGG

GTCGTGGTATGCTATGACCCGAAAGCGTTCCGAGGGAGCCGTGGTCAACGTCCAGCCAACTGACAAAGAA

TTCACTGTTCGGCTGGAGACAGAGAAGCGGCTGCACACGGTGGGCGAGCCGGTGGAGTTCAGATGCATCC

TGGAGGCTCAGAATGTTCCCGACCGTTACTTTGCTGTCTCCTGGGCCTTCAACAGCTCGCTCATCGCCAC

CATGGGTCCTAACGCTGTGCCTGTCCTCAACAGCGAATTTGCTCACCGGGAAGCCAGGGGACAGCTTAAG

GTGGCCAAAGAGAGCGACAGTGTCTTTGTGCTGAAGATCTACCACCTCCGCCAGGAAGATAGCGGGAAAT

ACAACTGCCGGGTGACTGAGCGAGAGAAAACCGTGACCGGGGAATTCATTGATAAGGAGAGCAAGCGTCC

CAAGAACATCCCCATCATAGTCCTCCCCCTCAAGAGCAGCATCTCCGTGGAGGTGGCCAGCAATGCCAGC

GTCATCCTTGAGGGCGAGGACCTGCGCTTCTCCTGCAGTGTCCGCACGGCAGGCAGGCCGCAGGGTCGCT

TCTCTGTCATCTGGCAGCTTGTGGACAGGCAGAACCGCCGCAGCAATATCATGTGGCTAGACCGGGATGG

CACCGTGCAGCCAGGCTCGTCCTACTGGGAGCGCAGCAGCTTTGGGGGCGTCCAGATGGAGCAGGTGCAG

CCCAACTCGTTCAGCCTGGGCATCTTCAACAGCAGGAAGGAGGACGAGGGCCAGTATGAATGCCATGTGA

CTGAATGGGTGCGGGCAGTGGATGGCGAGTGGCAGATTGTTGGGGAGCGCCGGGCCAGCACTCCCATCTC

CATCACAGCTCTTGAAATGGGCTTCGCAGTCACAGCCATCTCCCGGACACCGGGGGTGACCTACAGCGAC

TCCTTTGACTTGCAGTGTATCATCAAACCCCACTACCCTGCCTGGGTCCCCGTGTCGGTGACATGGCGGT

TCCAGCCGGTGGGCACGGTGGAGTTCCATGACTTGGTGACCTTCACCCGGGACGGAGGGGTCCAGTGGGG

GGACAGGTCCTCCAGCTTCCGAACCCGAACTGCCATCGAGAAGGCTGAGTCCAGCAACAACGTCCGCCTA

AGCATCAGCCGAGCCAGTGACACGGAAGCAGGCAAGTACCAGTGTGTGGCAGAGCTGTGGCGGAAGAACT

ACAACAACACCTGGACGCGACTGGCGGAGAGGACCTCCAACCTGCTGGAGATCAGGGTGCTGCAGCCAGT

GACAAAGCTGCAGGTGAGCAAATCGAAGAGGACCCTCACCCTGGTGGAAAACAAGCCCATTCAGTTGAAC

TGCTCAGTCAAGTCTCAGACTAGCCAGAACTCCCACTTTGCGGTGCTCTGGTATGTCCACAAGCCCTCGG

ATGCCGATGGCAAGCTTATCCTGAAGACCACCCACAACTCCGCCTTTGAATACGGTACTTACGCCGAGGA

GGAGGGCCTGAGAGCCAGGCTCCAGTTTGAGAGGCATGTGTCGGGGGGCCTGTTCAGCCTCACCGTCCAG

AGAGCCGAGGTCAGCGACAGCGGCAGCTACTACTGCCACGTGGAGGAGTGGCTGCTGAGCCCCAACTACG

CCTGGTACAAGCTGGCAGAGGAGGTTTCTGGGCGCACAGAAGTCACTGTGAAACAGCCAGACAGCCGCCT

GAGGCTCAGCCAAGCCCAGGGGAACCTGTCGGTTCTGGAGACCCGGCAGGTACAGCTGGAGTGTGTGGTT

CTCAACCGCACCAGCATAACCTCCCAGCTCATGGTGGAATGGTTTGTATGGAAGCCCAACCACCCTGAGC

GGGAGACTGTGGCCCGCTTGAGCCGTGACGCCACCTTCCACTATGGAGAGCAGGCAGCCAAGAACAATCT

GAAGGGGCGGCTGCATTTGGAGAGTCCTTCCCCCGGCGTGTACCGTCTCTTCATCCAGAACGTGGCTGTG

CAGGACAGCGGGACCTACAGCTGCCATGTGGAGGAGTGGCTGCCCAGCCCCAGTGGCATGTGGTATAAGC

GGGCAGAGGACACCGCTGGGCAGACAGCTCTGACAGTCATGCGACCAGATGCTTCCCTGCAGGTGGACAC

AGTGGTCCCCAATGCCACGGTCTCTGAGAAGGCAGCTTTCCAGCTGGACTGTAGCATCGTGTCCCGCTCC

AGCCAGGACTCCCGCTTCGCTGTGGCCTGGTATTCCCTGAGGACTAAAGCTGGGGGGAAAAGGAGCAGCC

CTGGCCTGGAAGAACAGGAAGAGGAAAGGGAGGAGGAGGAGGAGGAGGACGACGACGACGACGACGACCC

AACAGAGCGGACGGCCCTGCTGAGCGTGGGCCCAGATGCTGTCTTTGGCCCAGAGGGCAGTCCTTGGGAG

GGCAGGCTTCGCTTCCAGAGGCTCTCCCCGGTGCTCTACCGGCTCACAGTGCTGCAGGCAAGCCCCCAAG

ATACAGGCAATTACTCCTGCCATGTGGAGGAGTGGCTGCCCAGCCCTCAGAAGGAATGGTACCGGCTGAC

GGAGGAGGAGTCAGCCCCCATCGGCATCCGTGTTCTAGATACAAGTCCCACCCTCCAGTCCATCATCTGC

TCCAACGACGCACTCTTCTACTTCGTCTTCTTCTACCCTTTCCCCATCTTTGGCATTCTTATCATCACCA

TCCTTCTGGTGCGTTTCAAGAGCCGGAACTCCAGCAAGAACTCTGATGGGAAGAATGGGGTGCCTCTGCT

GTGGATCAAAGAGCCACACCTCAACTACTCCCCTACTTGCCTGGAGCCCCCTGTTCTCAGTATCCATCCA

GGGGCCATAGACTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.