Human IgSF2/CD101/EWI101 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

IgSF2 or CD101 is a 1021 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the EWI subfamily of the Immunoglobulin superfamily (IgSF).  IgSF2 contains an N-terminal signal peptide, a 934-aa extracellular domain (ECD) with 7 Ig-like C2-type domains, an EWI (Glu-Trp-Ile)-motif and 2 potential glycosylation sites, a transmembrane domain (TM), and a 46-aa cytoplasmic tail. IgSF2 plays a role as inhibitor of T-cells proliferation induced by CD3 via IL-10 secretion by cutaneous dendritic cells. IgSF2 inhibits expression of IL2RA on activated T-cells and secretion of IL2. IgSF2 inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. It also inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. IgSF2 prevents nuclear translocation of nuclear factor of activated T-cell (NFAT) to the nucleus. IgSF2 may be a marker of CD4+ CD56+ leukemic tumor cells.

 

 

Gene Symbol: IgSF2; CD101; EWI101; V7; EWI-101; IGSF-2

 

NCBI Gene ID: 9398

 

Uniprot Entry: Q93033

 

Construct Details: Full length human IgSF2/CD101 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3066 (bp)

 

Gene Insert Sequence:  

ATGGCAGGCATCTCATATGTGGCATCTTTCTTTCTCCTTCTGACTAAGCTCAGCATTGGCCAGAGAGAAG

TAACAGTTCAGAAAGGACCACTGTTTAGAGCTGAAGGTTACCCAGTCAGCATTGGCTGCAATGTAACTGG

CCACCAGGGACCTTCTGAGCAGCATTTCCAGTGGTCTGTTTACCTGCCGACAAACCCGACCCAGGAAGTC

CAGATCATTAGCACCAAGGATGCTGCCTTCTCTTACGCAGTATATACGCAGCGGGTGCGAAGCGGAGACG

TCTACGTGGAGAGGGTCCAGGGCAACTCAGTCTTGTTGCACATCTCAAAACTCCAGATGAAGGATGCTGG

CGAGTATGAGTGTCACACACCAAACACTGATGAGAAATACTATGGAAGTTACAGTGCAAAGACTAATCTA

ATTGTTATTCCAGATACCCTCTCTGCCACCATGAGTTCTCAGACTCTCGGTAAGGAGGAAGGTGAGCCAT

TAGCCCTCACCTGTGAGGCATCCAAAGCCACAGCCCAACATACTCACCTCTCTGTCACCTGGTACCTAAC

ACAGGATGGAGGAGGAAGCCAAGCCACTGAGATTATTTCTCTCTCCAAAGATTTTATATTGGTCCCTGGG

CCCTTGTATACAGAGCGGTTTGCAGCCAGTGACGTACAGCTCAACAAACTGGGACCCACTACATTCAGGC

TGTCCATAGAGAGGCTCCAGTCCTCAGATCAGGGTCAGCTGTTCTGTGAGGCAACGGAATGGATTCAGGA

TCCAGATGAAACTTGGATGTTCATCACCAAAAAGCAGACCGATCAAACCACTCTGAGGATCCAGCCAGCA

GTGAAAGATTTTCAAGTCAACATTACAGCTGACAGCTTGTTTGCTGAAGGGAAACCCTTAGAACTGGTTT

GCCTGGTTGTAAGCAGTGGCCGTGACCCACAGCTTCAAGGCATTTGGTTCTTCAATGGGACTGAAATTGC

TCACATTGATGCTGGTGGAGTCCTGGGCCTGAAGAATGACTACAAAGAGAGAGCAAGTCAAGGAGAGCTC

CAGGTTTCAAAGTTAGGCCCCAAGGCTTTCTCTCTCAAGATCTTCTCTCTGGGCCCAGAGGATGAAGGCG

CCTACAGATGTGTGGTAGCAGAGGTCATGAAAACACGCACAGGTTCCTGGCAGGTGCTTCAGAGAAAGCA

GTCACCAGACAGCCACGTGCACCTGAGGAAGCCAGCAGCAAGAAGTGTGGTCATGTCTACCAAGAACAAG

CAGCAAGTTGTGTGGGAAGGAGAGACACTCGCCTTTCTCTGTAAGGCTGGTGGAGCTGAAAGTCCCCTGT

CTGTGAGCTGGTGGCACATCCCACGGGACCAGACACAGCCCGAGTTTGTGGCTGGCATGGGGCAGGATGG

CATTGTGCAGCTGGGTGCCTCCTATGGGGTACCCAGTTACCATGGCAACACAAGGCTGGAGAAAATGGAC

TGGGCCACCTTCCAGCTGGAGATCACCTTCACTGCCATCACAGACAGTGGCACATATGAGTGCAGAGTAT

CTGAGAAGTCTCGGAACCAGGCCAGAGATCTGAGCTGGACTCAGAAGATTTCAGTTACTGTAAAGTCTCT

GGAGTCAAGTTTACAAGTTAGTCTGATGAGCCGTCAGCCGCAAGTGATGTTAACCAACACCTTTGACCTG

TCCTGTGTCGTGAGGGCCGGTTACTCTGACCTCAAGGTGCCACTCACTGTGACGTGGCAGTTCCAGCCAG

CTAGCTCTCACATCTTCCACCAGCTTATTCGAATCACCCACAATGGCACTATTGAATGGGGGAATTTCCT

ATCCCGGTTCCAAAAGAAGACGAAAGTGTCGCAGTCTTTATTTCGTTCACAACTCCTAGTCCATGATGCC

ACTGAGGAAGAGACAGGAGTGTATCAGTGTGAAGTAGAAGTTTATGACAGAAATTCCCTATACAACAACC

GCCCCCCGAGGGCTTCTGCCATCTCTCACCCACTGAGGATAGCCGTCACTTTACCAGAGAGCAAGCTAAA

AGTGAATTCAAGGAGTCAAGTCCAAGAGCTCTCCATCAACTCCAACACTGATATAGAATGTAGCATCTTG

TCCCGGTCCAATGGAAACCTTCAGTTAGCCATTATTTGGTATTTTTCTCCTGTTTCCACTAATGCCTCTT

GGCTAAAGATCCTGGAGATGGACCAAACCAATGTTATAAAAACTGGGGATGAGTTTCACACCCCACAGAG

AAAACAAAAATTTCATACTGAGAAGGTTTCCCAAGACTTATTTCAGCTGCACATTCTGAATGTGGAAGAC

AGCGATCGGGGCAAATATCACTGTGCTGTGGAGGAATGGCTCCTGTCTACAAATGGCACTTGGCACAAGC

TTGGAGAAAAGAAGTCAGGACTAACAGAATTGAAACTCAAGCCCACAGGAAGTAAGGTACGTGTCTCCAA

AGTGTACTGGACCGAAAATGTGACTGAGCACAGAGAAGTGGCCATCCGCTGCAGCCTGGAGAGTGTAGGC

AGCTCAGCCACTCTGTACTCTGTGATGTGGTACTGGAACAGAGAAAACTCTGGAAGTAAATTGCTGGTGC

ACTTGCAACATGATGGCTTGCTGGAGTATGGGGAAGAGGGGCTCAGGAGGCACCTGCACTGTTACCGTTC

ATCCTCTACAGACTTTGTCCTGAAGCTTCATCAGGTGGAGATGGAGGATGCAGGAATGTACTGGTGTAGG

GTGGCAGAGTGGCAGCTCCATGGACACCCAAGCAAGTGGATTAATCAAGCATCCGATGAGTCACAGCGGA

TGGTGCTCACGGTGCTGCCTTCAGAGCCCACGCTTCCTTCCAGGATCTGCTCCTCGGCCCCTTTACTCTA

TTTCCTGTTCATCTGTCCCTTCGTCCTGCTCCTCCTTCTGCTCATCTCCCTCCTCTGCTTATACTGGAAG

GCCAGGAAGTTGTCAACACTGCGTTCCAACACACGGAAAGAAAAAGCTCTCTGGGTGGACTTGAAAGAGG

CTGGAGGTGTGACCACAAATAGGAGGGAAGACGAGGAGGAAGATGAAGGCAACTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.