Human CDHR2/PCLKC/PCDH24 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  CDHR2 (Cadherin-related family member 2) is an 1310 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDHR2 contains an N-terminal signal peptide, a 1134-aa extracellular domain (ECD) with 9 cadherin domains and 24 potential glycosylation sites, a transmembrane domain (TM), and a 135-aa cytoplasmic tail. CDHR2 is a member of the protocadherin family, a subset of the cadherin superfamily. The members of the protocadherin family encode non-classical cadherins that function as calcium-dependent cell-cell adhesion molecules. CDHR2 is a intermicrovillar adhesion molecule that forms, via its extracellular domain, calcium-dependent heterophilic complexes with CDHR5 on adjacent microvilli. CDHR2 may also play a role in cell-cell adhesion and contact inhibition in epithelial cells as well as in tumor suppression.

 

 

Gene Symbol: CDHR2; PCLKC; PCDH24

 

NCBI Gene ID: 54825

 

Uniprot Entry: Q9BYE9

 

Construct Details: Full length human CDHR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3933(bp)

 

Gene Insert Sequence:  

ATGGCCCAGCTATGGCTGTCCTGCTTCCTCCTTCCTGCCCTCGTGGTGTCTGTGGCAGCCAACGTGGCCC

CGAAGTTCCTAGCCAACATGACGTCAGTGATCCTGCCTGAGGACCTGCCTGTGGGTGCCCAGGCCTTCTG

GTTGGTAGCGGAAGACCAGGACAATGACCCTCTGACCTATGGGATGAGCGGCCCCAATGCCTACTTCTTC

GCTGTCACTCCGAAAACTGGGGAAGTGAAGCTGGCCAGCGCTCTGGACTACGAGACACTCTACACATTCA

AAGTCACCATCTCCGTGAGCGACCCCTACATCCAGGTGCAGAGGGAGATGCTGGTGATTGTGGAAGATAG

AAACGACAACGCACCCGTTTTCCAGAACACCGCTTTCTCCACCAGCATCAACGAGACCCTGCCCGTGGGC

AGTGTGGTGTTCTCCGTGCTGGCCGTGGATAAAGACATGGGGTCTGCAGGCATGGTCGTGTACTCCATAG

AGAAGGTCATCCCTAGCACTGGGGACAGCGAGCATCTCTTCCGGATCCTGGCCAATGGCTCCATAGTCCT

CAATGGCAGCCTCAGCTACAACAACAAGAGCGCTTTCTACCAGCTGGAGCTGAAGGCCTGTGACTTGGGC

GGCATGTACCACAACACCTTCACCATCCAGTGCTCCCTGCCTGTCTTCCTGTCCATCTCCGTGGTGGACC

AGCCTGACCTTGACCCCCAGTTTGTCAGGGAGTTTTACTCGGCCTCTGTGGCTGAGGATGCAGCCAAGGG

AACCTCGGTGCTGACGGTGGAGGCTGTGGATGGCGACAAAGGCATCAATGACCCTGTGATCTACAGCATC

TCCTACTCCACGCGGCCCGGCTGGTTTGACATCGGGGCAGATGGGGTGATCAGGGTCAACGGCTCCCTGG

ACCGTGAGCAGCTGCTGGAGGCGGATGAGGAGGTGCAGCTGCAGGTCACGGCCACCGAGACACACCTCAA

CATCTACGGGCAGGAGGCCAAGGTGAGCATCTGGGTGACAGTGAGAGTGATGGACGTCAATGACCACAAA

CCTGAGTTTTACAACTGCAGCCTCCCAGCCTGCACCTTCACCCCCGAAGAGGCCCAAGTGAACTTCACTG

GCTACGTGGACGAGCATGCCTCCCCCCGCATCCCCATCGATGACCTCACCATGGTGGTCTACGACCCGGA

CAAGGGCAGCAATGGCACCTTCCTGTTGTCGCTGGGGGGCCCCGATGCAGAAGCCTTCAGCGTCTCCCCG

GAGCGGGCAGTGGGCTCAGCCTCCGTTCAGGTGCTGGTGAGAGTATCCGCGCTGGTGGACTACGAGAGGC

AGACGGCGATGGCGGTGCAGGTTGTGGCCACAGACTCCGTCAGCCAGAACTTCTCCGTCGCCATGGTGAC

CATCCACCTTAGAGACATTAATGACCACAGGCCCACGTTTCCCCAGAGCTTGTACGTCCTCACGGTGCCA

GAGCACAGCGCCACCGGCTCTGTGGTCACCGACAGCATCCACGCCACGGACCCAGACACGGGCGCGTGGG

GCCAAATTACCTACAGCCTGCTCCCAGGAAATGGGGCAGACCTCTTCCAAGTGGATCCCGTCTCAGGGAC

GGTGACGGTGAGGAACGGTGAGCTGCTGGACCGGGAGAGCCAGGCCGTGTACTACCTGACGCTGCAGGCC

ACAGACGGCGGGAACCTGTCCTCCTCCACCACACTGCAGATCCACCTGCTGGACATCAACGACAATGCAC

CCGTGGTTAGCGGCTCCTACAACATCTTCGTCCAGGAGGAGGAGGGCAATGTCTCCGTGACCATCCAGGC

CCACGACAATGATGAGCCGGGCACCAACAACAGCCGTCTGCTCTTCAACCTGCTGCCTGGCCCCTACAGC

CACAACTTCTCCTTGGACCCTGACACAGGGCTCCTCAGAAACCTGGGGCCCCTGGACAGAGAGGCCATCG

ACCCCGCCCTGGAGGGCCGCATTGTGCTGACAGTGCTTGTGTCTGACTGCGGCGAGCCTGTCCTCGGCAC

CAAAGTCAATGTCACCATCACTGTGGAGGACATCAATGATAACCTGCCCATCTTCAATCAGTCCAGCTAC

AACTTTACGGTGAAGGAGGAGGATCCAGGAGTGCTAGTGGGCGTGGTGAAGGCCTGGGACGCGGACCAGA

CGGAAGCCAACAACCGCATCAGCTTCAGCCTGTCGGGGAGTGGTGCCAACTACTTCATGATCCGAGGCTT

GGTGCTGGGGGCTGGGTGGGCTGAGGGCTACCTCCGGCTGCCCCCGGACGTGAGCCTGGATTACGAGACA

CAGCCCGTCTTCAACTTGACAGTGAGTGCTGAGAACCCAGACCCCCAGGGGGGTGAGACCATAGTAGACG

TCTGCGTGAATGTGAAAGACGTGAACGACAATCCCCCCACCCTGGATGTAGCCTCACTCCGGGGCATCCG

TGTGGCTGAGAATGGCTCACAGCACGGCCAGGTGGCTGTGGTGGTTGCCTCGGATGTGGACACCAGTGCC

CAGCTGGAGATACAGCTTGTGAACATTCTCTGCACCAAGGCCGGGGTCGATGTGGGCAGCCTATGCTGGG

GCTGGTTCTCAGTGGCGGCCAACGGCTCTGTGTACATCAACCAGAGCAAAGCCATCGACTACGAGGCCTG

TGACCTGGTCACGCTGGTTGTGCGGGCCTGTGACCTAGCCACGGACCCCGGCTTCCAGGCCTACAGCAAC

AATGGAAGCCTCCTCATTACCATTGAGGACGTGAATGACAATGCACCCTATTTTCTGCCTGAGAATAAGA

CTTTTGTGATCATCCCTGAACTCGTGCTGCCCAACCGGGAGGTGGCTTCTGTCCGGGCCAGAGACGATGA

TTCAGGGAACAATGGCGTCATCCTGTTCTCCATCCTCCGAGTAGACTTCATCTCTAAGGACGGGGCCACC

ATCCCTTTCCAGGGTGTCTTCTCGATCTTCACCTCCTCCGAGGCCGACGTGTTCGCTGGGAGCATTCAGC

CGGTGACCAGCCTCGACTCCACTCTCCAAGGCACCTACCAAGTGACAGTCCAGGCCAGGGACAGACCTTC

CTTGGGTCCTTTCCTGGAAGCCACCACCACCCTGAATCTCTTCACCGTGGACCAGAGTTACCGCTCGCGG

CTGCAGTTCTCCACACCGAAGGAGGAGGTGGGCGCCAACAGACAGGCGATTAATGCGGCTCTTACCCAGG

CAACCAGGACTACAGTATACATTGTGGACATTCAGGACATAGATTCTGCAGCTCGGGCCCGACCTCACTC

CTACCTCGATGCCTACTTTGTCTTCCCCAATGGGTCAGCCCTGACCCTTGATGAGCTGAGTGTGATGATC

CGGAATGATCAGGACTCGCTGACGCAGCTGCTGCAGCTGGGGCTGGTGGTGCTGGGCTCCCAGGAGAGCC

AGGAGTCAGACCTGTCGAAACAGCTCATCAGTGTCATCATAGGATTGGGAGTGGCTTTGCTGCTGGTCCT

TGTGATCATGACCATGGCCTTCGTGTGTGTGCGGAAGAGCTACAACCGGAAGCTTCAAGCTATGAAGGCT

GCCAAGGAGGCCAGGAAGACAGCAGCAGGGGTGATGCCCTCAGCCCCTGCCATCCCAGGGACTAACATGT

ACAACACTGAGCGAGCCAACCCCATGCTGAACCTCCCCAACAAAGACCTGGGCTTGGAGTACCTCTCTCC

CTCCAATGACCTGGACTCTGTCAGCGTCAACTCCCTGGACGACAACTCTGTGGATGTGGACAAGAACAGT

CAGGAAATCAAGGAGCACAGGCCACCACACACACCACCAGAGCCAGATCCAGAGCCCCTGAGCGTGGTCC

TGTTAGGACGGCAGGCAGGCGCAAGTGGACAGCTGGAGGGGCCATCCTACACCAACGCTGGCCTGGACAC

CACGGACCTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.