Human CDH28/CDHR3 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  CDHR3 (Cadherin-related family member 3) or CDH28 (Cadherin-like protein 28) is an 885 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH28 contains an N-terminal signal peptide, a 694-aa extracellular domain (ECD) with 6 cadherin domains and 3 potential glycosylation sites, a transmembrane domain (TM), and a 151-aa conderved cytoplasmic tail. CDH28 acts as a receptor for rhinovirus C.

 

 

Gene Symbol: CDH28; CDHR3

 

NCBI Gene ID: 222256

 

Uniprot Entry: Q6ZTQ4

 

Construct Details: Full length human CDH28 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2658 (bp)

 

Gene Insert Sequence:  

ATGCAGGAAGCAATCATTCTCCTGGCTCTCCTGGGTGCCATGTCAGGGGGAGAAGCACTACACCTAATCC

TCTTACCTGCTACAGGCAATGTGGCAGAGAATTCTCCACCTGGGACTTCAGTGCACAAGTTTTCTGTGAA

GTTATCAGCATCATTGTCACCTGTGATCCCAGGATTTCCCCAGATAGTCAACTCAAATCCCCTCACTGAA

GCTTTTAGGGTGAATTGGCTGTCAGGCACCTACTTTGAGGTTGTCACCACTGGGATGGAACAACTAGATT

TTGAAACAGGACCAAACATATTTGATTTGCAGATTTATGTGAAGGATGAGGTTGGTGTCACAGACCTGCA

AGTCCTGACTGTCCAGGTAACAGATGTGAACGAGCCACCTCAGTTTCAAGGCAACTTGGCAGAAGGTCTA

CACCTCTACATAGTAGAAAGAGCAAACCCTGGATTCATTTACCAGGTTGAGGCCTTCGATCCAGAAGACA

CAAGCCGAAACATTCCCCTCAGTTATTTCCTGATTTCTCCCCCAAAGAGCTTCAGAATGTCTGCTAATGG

CACCCTCTTCTCCACAACAGAATTGGACTTTGAAGCAGGACACAGAAGTTTCCATCTCATCGTGGAGGTG

AGGGACAGTGGAGGCCTCAAAGCCTCCACAGAGCTCCAGGTGAACATCGTGAACCTCAACGACGAAGTCC

CTCGCTTTACCAGCCCGACACGAGTGTACACAGTCCTGGAGGAACTGAGTCCAGGAACCATCGTGGCCAA

TATCACAGCGGAGGATCCTGATGATGAAGGTTTTCCCAGCCACCTCCTCTACAGCATTACCACTGTTAGC

AAATATTTCATGATAAATCAGTTGACTGGTACAATCCAAGTGGCCCAAAGGATAGACCGAGATGCAGGTG

AATTGAGACAAAATCCCACCATTTCCCTGGAAGTTCTAGTGAAGGACAGACCATATGGGGGTCAGGAGAA

TCGCATCCAGATAACCTTCATTGTGGAAGACGTCAACGACAATCCTGCCACATGCCAAAAGTTCACCTTC

AGCATTATGGTGCCGGAAAGAACAGCCAAGGGGACGTTGCTTCTTGACCTAAACAAGTTCTGCTTTGATG

ATGACAGTGAGGCACCAAACAACAGATTCAACTTCACCATGCCATCTGGAGTGGGGAGCGGCAGCAGATT

TTTACAGGATCCAGCTGGCTCTGGGAAGATTGTGCTGATTGGTGATCTAGACTACGAAAATCCAAGTAAC

CTAGCAGCCGGCAATAAATATACGGTGATAATCCAGGTGCAGGATGTGGCCCCCCCTTACTATAAAAATA

ACGTCTACGTTTATATCCTAACAAGCCCAGAAAATGAGTTTCCTCTCATTTTTGATAGGCCATCCTATGT

ATTTGATGTGTCAGAAAGAAGGCCCGCCAGAACCCGAGTGGGACAGGTGCGAGCCACTGATAAAGACCTC

CCCCAGAGCAGCCTCCTGTACTCCATCTCCACTGGAGGGGCCAGCCTCCAGTATCCAAATGTATTTTGGA

TTAATCCCAAGACAGGAGAACTCCAGCTGGTAACTAAAGTGGACTGTGAAACAACCCCCATCTATATTCT

CAGAATCCAGGCCACCAACAACGAAGACACAAGCTCTGTCACTGTTACTGTGAACATCCTTGAAGAAAAT

GATGAAAAGCCAATTTGTACTCCAAACTCTTATTTCCTGGCCCTCCCAGTGGATCTGAAAGTTGGCACAA

ATATTCAGAATTTCAAGCTGACATGTACCGACCTTGATTCCAGCCCCAGATCTTTCCGTTATTCCATTGG

CCCAGGTAACGTCAACAATCATTTCACCTTCTCTCCCAATGCTGGTTCCAATGTCACACGCCTGCTGCTT

ACATCTCGCTTTGACTATGCTGGTGGGTTTGATAAGATCTGGGACTACAAGCTACTTGTCTACGTAACTG

ATGACAACTTGATGTCTGACAGGAAGAAAGCGGAGGCTCTTGTTGAGACAGGAACAGTGACACTGAGTAT

TAAAGTCATTCCCCACCCAACCACTATCATCACCACGACCCCCAGGCCCAGGGTCACCTATCAGGTCCTG

AGGAAAAACGTTTACTCTCCATCTGCATGGTACGTGCCGTTTGTCATCACTTTGGGCTCCATATTGCTTC

TGGGTCTCCTCGTGTACCTGGTCGTCCTATTGGCCAAAGCCATCCACAGACACTGCCCCTGCAAGACTGG

GAAGAACAAGGAACCTCTGACAAAGAAAGGAGAAACGAAGACTGCAGAGAGAGACGTCGTGGTGGAAACT

ATCCAGATGAACACTATCTTTGATGGAGAAGCCATAGATCCAGTGACCGGGGAAACATATGAATTCAACT

CAAAAACTGGAGCCAGAAAGTGGAAAGATCCACTAACCCAAATGCCAAAATGGAAAGAGTCCAGCCACCA

GGGAGCTGCCCCACGCAGAGTCACTGCTGGGGAAGGGATGGGGTCACTGAGAAGTGCCAACTGGGAAGAA

GATGAGCTGAGTGGCAAAGCGTGGGCTGAGGATGCTGGTCTGGGTTCCAGAAATGAGGGTGGCAAGCTGG

GCAACCCAAAGAACAGAAATCCAGCCTTCATGAACAGGGCTTACCCCAAACCACACCCAGGAAAGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.