Human CDH22/Cadherin22 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin22 or CDH22 is an 828 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH22 contains an N-terminal signal peptide, a 589-aa extracellular domain (ECD) with 5 cadherin domains and 3 potential glycosylation sites, a transmembrane domain (TM), and a 183-aa conserved cytoplasmic tail. CDH22 is expressed predominantly in the brain and may play an important role in morphogenesis and tissue formation in neural and non-neural cells during development and maintenance of the brain and neuroendocrine organs. 

 

 

Gene Symbol: CDH22; Cadherin-22; PB-cadherin; C20orf25; dJ998H6.1; Cadherin22

 

NCBI Gene ID: 64405

 

Uniprot Entry: Q9UJ99

 

Construct Details: Full length human CDH22 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2487 (bp)

 

Gene Insert Sequence:  

ATGAGGCCGAGGCCCGAAGGTAGGGGGCTCCGGGCGGGAGTCGCGCTGTCCCCCGCGCTACTGCTGCTGC

TGCTGCTGCCGCCGCCGCCGACGCTGCTGGGGCGCCTGTGGGCAGCGGGCACACCCTCGCCGTCGGCGCC

CGGAGCTCGGCAGGACGGCGCGCTGGGAGCCGGCCGCGTCAAACGCGGCTGGGTGTGGAACCAGTTCTTC

GTGGTAGAGGAGTACACGGGCACGGAGCCCCTGTATGTGGGCAAGATCCACTCCGACTCAGACGAGGGTG

ACGGGGCCATCAAGTACACCATCTCAGGCGAGGGTGCTGGGACCATCTTCCTGATCGACGAGCTGACAGG

CGACATTCATGCCATGGAGCGCCTGGACCGCGAGCAGAAAACCTTCTACACGCTGCGGGCCCAGGCTCGG

GATCGCGCCACCAACCGCCTACTGGAGCCCGAGTCGGAGTTCATCATCAAGGTGCAGGACATCAATGACA

GTGAGCCCCGCTTCCTGCACGGCCCCTATATTGGCAGCGTGGCCGAGCTCTCACCTACAGGCACGTCGGT

GATGCAGGTGATGGCCTCGGATGCGGATGACCCCACGTACGGCAGCAGCGCTCGGCTGGTGTACAGCGTG

CTGGACGGCGAGCACCACTTCACCGTGGACCCCAAGACCGGCGTAATCCGGACGGCTGTGCCTGACCTTG

ACCGCGAGAGCCAGGAGCGCTACGAGGTGGTGATCCAGGCCACAGACATGGCGGGTCAGCTGGGTGGCCT

CTCGGGCTCCACTACCGTCACCATCGTAGTCACCGACGTCAATGACAACCCGCCCCGTTTCCCGCAGAAG

ATGTACCAGTTCAGCATCCAGGAGTCAGCCCCCATTGGAACGGCTGTGGGACGTGTGAAGGCTGAGGACT

CAGACGTGGGAGAGAACACAGACATGACTTACCACCTTAAGGACGAGAGCAGCAGCGGCGGCGATGTGTT

CAAGGTCACCACAGACAGCGACACTCAGGAGGCCATCATCGTAGTGCAGAAGCGCCTGGACTTCGAATCC

CAGCCCGTGCACACCGTGATCCTGGAGGCCCTCAACAAGTTCGTGGACCCCCGCTTCGCCGACCTGGGCA

CGTTCCGCGACCAGGCGATCGTGCGCGTGGCCGTGACCGACGTGGACGAGCCCCCCGAGTTCCGGCCGCC

CTCCGGCCTCCTGGAGGTGCAGGAGGACGCGCAGGTGGGCTCCCTGGTCGGCGTGGTGACGGCGCGGGAC

CCCGACGCCGCCAACCGGCCCGTCCGGTACGCCATTGACCGCGAATCAGATTTGGACCAGATCTTCGATA

TCGATGCGGACACAGGCGCCATCGTGACTGGCAAGGGGCTGGACCGCGAGACGGCCGGCTGGCACAACAT

CACAGTGCTGGCCATGGAGGCGGACAATCATGCACAGCTATCCCGGGCATCCCTAAGGATCCGAATCCTG

GATGTGAACGACAATCCCCCAGAACTGGCCACACCCTACGAGGCAGCTGTATGCGAGGATGCCAAGCCAG

GCCAGCTCATCCAGACCATCAGCGTGGTGGACAGAGACGAGCCCCAAGGCGGGCACCGCTTCTATTTCCG

CCTGGTGCCTGAAGCTCCCAGCAACCCTCATTTCTCTCTGCTTGACATCCAAGACAACACCGCTGCAGTG

CACACGCAGCACGTGGGCTTCAACCGGCAGGAGCAGGACGTGTTCTTCCTGCCCATCCTGGTGGTAGACA

GTGGGCCGCCCACACTGAGCAGCACAGGCACGCTCACCATCCGCATCTGTGGCTGCGACAGCTCCGGCAC

CATCCAGTCCTGCAACACCACGGCCTTTGTCATGGCCGCCTCCCTCAGCCCCGGCGCCCTCATCGCCCTC

TTGGTCTGCGTTCTCATCCTGGTTGTGCTGGTGCTGCTGATCCTCACCCTCAGGCGCCACCACAAGAGCC

ACCTGAGCTCGGACGAGGATGAAGACATGCGGGACAACGTCATCAAATACAACGACGAAGGCGGCGGCGA

GCAGGACACCGAAGCCTACGACATGTCGGCGCTGCGGAGCCTCTACGACTTCGGCGAGCTCAAGGGCGGC

GACGGGGGCGGCAGCGCGGGCGGGGGAGCGGGCGGGGGCTCGGGCGGGGGCGCGGGCAGCCCCCCGCAGG

CCCACCTGCCCTCCGAGCGCCACTCGCTGCCGCAGGGGCCGCCGAGCCCCGAGCCAGACTTCTCAGTGTT

CAGGGACTTCATCAGCCGCAAGGTGGCACTGGCGGACGGGGACCTGTCGGTGCCGCCCTACGACGCCTTC

CAGACCTACGCCTTCGAGGGCGCGGACTCGCCGGCCGCCTCGCTCAGCTCCCTGCACAGCGGCTCGTCGG

GCTCCGAGCAGGACTTCGCCTATCTCAGCAGCTGGGGTCCGCGCTTCCGGCCCCTGGCCGCGCTCTACGC

CGGCCACCGCGGGGACGACGAGGCCCAGGCCTCCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.