Human CDH18/CDH14/Cadherin18 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin18 or CDH18 is a 790 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH18 contains an N-terminal signal peptide, a 555-aa extracellular domain (ECD) with 5 cadherin domains and 4 potential glycosylation sites, a transmembrane domain (TM), and a 154-aa conderved cytoplasmic tail. CDH18 is a type II cadherin that may play an important role in cell differentiation and morphogenesis. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins.  CDH18 is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance. 

 

 

Gene Symbol: CDH18; Cadherin-18; Cadherin-14; CDH14; CDH24; CDH14L; Cadherin18

 

NCBI Gene ID: 1016

 

Uniprot Entry: Q13634

 

Construct Details: Full length human CDH18 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2373 (bp)

 

Gene Insert Sequence:  

ATGAAAATTACTAGCACATCTTGCATCTGTCCAGTCCTAGTGTGTCTCTGTTTTGTGCAGAGGTGTTATG

GAACTGCTCACCACAGCTCCATCAAGGTGATGAGAAACCAAACCAAACACATTGAAGGTGAAACCGAAGT

CCATCATCGTCCCAAAAGGGGATGGGTATGGAATCAGTTCTTTGTTTTAGAAGAACATATGGGACCAGAT

CCTCAGTATGTTGGAAAGCTGCACTCCAATTCTGACAAAGGTGATGGATCTGTCAAGTACATCCTTACTG

GAGAGGGTGCTGGGACTATATTTATCATTGACGATACCACGGGTGATATCCACTCAACAAAAAGCCTAGA

CAGAGAGCAGAAGACCCACTATGTGCTTCATGCTCAAGCTATTGATAGACGTACAAACAAACCTCTTGAG

CCTGAATCCGAGTTCATCATCAAAGTGCAAGACATCAATGACAACGCTCCAAAATTCACAGATGGACCAT

ACATTGTTACTGTGCCTGAAATGTCAGATATGGGTACCTCTGTTCTACAGGTGACAGCTACTGATGCAGA

TGACCCTACCTATGGAAACAGCGCTCGGGTGGTTTACAGCATTCTCCAAGGACAACCCTACTTCTCCGTC

GACCCTAAAACAGGAGTTATTAGAACGGCCTTACATAACATGGACAGAGAAGCCAGAGAACATTACTCCG

TAGTCATTCAAGCCAAAGACATGGCTGGGCAAGTTGGAGGGCTTTCAGGATCTACAACAGTCAACATCAC

CTTAACCGATGTCAATGACAACCCACCACGCTTTCCTCAAAAACACTATCAGCTATATGTTCCTGAGTCA

GCTCAAGTTGGTTCAGCTGTTGGGAAAATCAAGGCAAATGATGCTGACACTGGCTCAAATGCTGACATGA

CCTACTCCATCATAAATGGTGATGGCATGGGAATATTCTCAATCTCCACTGACAAAGAGACCAGAGAAGG

AATCCTTTCTTTAAAGAAGCCACTGAACTATGAGAAAAAGAAGTCATATACCCTCAACATAGAAGGAGCA

AATACACATCTTGATTTTCGCTTTTCTCACTTGGGTCCTTTTAAAGATGCTACTATGCTGAAGATCATTG

TTGGGGATGTAGATGAACCACCACTATTTTCCATGCCTTCCTACCTCATGGAAGTCTACGAAAATGCCAA

GATTGGGACCGTCGTTGGTACAGTTTTGGCACAAGATCCTGACAGTACTAACAGCTTAGTAAGATACTTC

ATCAACTACAATGTTGAAGACGACAGATTTTTCAACATTGATGCCAATACTGGGACCATTAGGACTACAA

AGGTTCTCGACAGAGAAGAAACTCCATGGTACAACATCACAGTCACTGCTTCAGAAATTGATAATCCTGA

TTTGCTGAGCCATGTCACAGTGGGTATTAGAGTTCTGGATGTCAATGACAATCCACCCGAACTTGCCAGG

GAATATGATATTATTGTATGTGAAAATTCTAAGCCTGGCCAGGTTATTCATACCATCAGTGCCACTGATA

AAGATGATTTTGCCAATGGACCAAGGTTTAACTTCTTTCTTGATGAACGCCTGCCTGTAAATCCAAACTT

CACTCTGAAGGACAATGAAGATAACACAGCCAGCATTCTGACAAGGCGGAGGAGATTTAGTCGAACTGTT

CAGGATGTGTATTATCTGCCCATTATGATCTCTGATGGTGGAATCCCCTCTCTCAGCAGCAGCAGCACCC

TCACCATCAGGGTTTGTGCATGCGAGAGAGATGGGCGTGTGCGGACCTGCCATGCAGAAGCCTTCCTGTC

CTCGGCTGGTTTGAGTACAGGAGCCTTAATCGCTATTCTTCTCTGTGTTCTCATTCTCCTGGCAATTGTG

GTACTTTTTATCACCCTGAGGCGCAGCAAAAAAGAGCCCTTGATCATTTCAGAAGAGGATGTACGGGAGA

ACGTGGTCACCTATGATGATGAAGGAGGCGGAGAGGAAGACACAGAGGCCTTTGACATCACAGCCTTGAG

GAATCCTTCTGCTGCTGAGGAGCTCAAGTACCGGAGGGATATCAGACCTGAAGTGAAGCTCACTCCCAGA

CACCAGACATCATCCACCCTGGAAAGCATAGATGTTCAGGAATTTATTAAGCAAAGACTGGCAGAAGCAG

ACCTAGACCCTAGCGTTCCCCCTTATGACTCTCTTCAGACTTATGCCTATGAGGGTCAGAGATCAGAAGC

TGGGTCTATCAGCTCGCTGGATTCAGCAACGACACAATCAGACCAGGATTATCACTACCTTGGAGACTGG

GGACCCGAGTTTAAAAAGTTAGCTGAACTCTATGGAGAAATAGAATCTGAAAGAACAACTTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.