Human CDH17/HPT1/Cadherin17 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin17 or CDH17 is an 832 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH17 contains an N-terminal signal peptide, a 765-aa extracellular domain (ECD) with 7 cadherin domains and 8 potential glycosylation sites, a transmembrane domain (TM), and a 24-aa short cytoplasmic tail. CDH17 is expressed in the gastrointestinal tract and pancreatic duct.  CDH17 is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. CDH17 may also play a role in the morphological organization of liver and intestine. 

 

 

Gene Symbol: CDH17; Cadherin-17; LI-cadherin; HPT1; HPT-1; Cadherin17

 

NCBI Gene ID: 1015

 

Uniprot Entry: Q12864

 

Construct Details: Full length human CDH17 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2499 (bp)

 

Gene Insert Sequence:  

ATGATACTTCAGGCCCATCTTCACTCCCTGTGTCTTCTTATGCTTTATTTGGCAACTGGATATGGCCAAG

AGGGGAAGTTTAGTGGACCCCTGAAACCCATGACATTTTCTATTTATGAAGGCCAAGAACCGAGTCAAAT

TATATTCCAGTTTAAGGCCAATCCTCCTGCTGTGACTTTTGAACTAACTGGGGAGACAGACAACATATTT

GTGATAGAACGGGAGGGACTTCTGTATTACAACAGAGCCTTGGACAGGGAAACAAGATCTACTCACAATC

TCCAGGTTGCAGCCCTGGACGCTAATGGAATTATAGTGGAGGGTCCAGTCCCTATCACCATAAAAGTGAA

GGACATCAACGACAATCGACCCACGTTTCTCCAGTCAAAGTACGAAGGCTCAGTAAGGCAGAACTCTCGC

CCAGGAAAGCCCTTCTTGTATGTCAATGCCACAGACCTGGATGATCCGGCCACTCCCAATGGCCAGCTTT

ATTACCAGATTGTCATCCAGCTTCCCATGATCAACAATGTCATGTACTTTCAGATCAACAACAAAACGGG

AGCCATCTCTCTTACCCGAGAGGGATCTCAGGAATTGAATCCTGCTAAGAATCCTTCCTATAATCTGGTG

ATCTCAGTGAAGGACATGGGAGGCCAGAGTGAGAATTCCTTCAGTGATACCACATCTGTGGATATCATAG

TGACAGAGAATATTTGGAAAGCACCAAAACCTGTGGAGATGGTGGAAAACTCAACTGATCCTCACCCCAT

CAAAATCACTCAGGTGCGGTGGAATGATCCCGGTGCACAATATTCCTTAGTTGACAAAGAGAAGCTGCCA

AGATTCCCATTTTCAATTGACCAGGAAGGAGATATTTACGTGACTCAGCCCTTGGACCGAGAAGAAAAGG

ATGCATATGTTTTTTATGCAGTTGCAAAGGATGAGTACGGAAAACCACTTTCATATCCGCTGGAAATTCA

TGTAAAAGTTAAAGATATTAATGATAATCCACCTACATGTCCGTCACCAGTAACCGTATTTGAGGTCCAG

GAGAATGAACGACTGGGTAACAGTATCGGGACCCTTACTGCACATGACAGGGATGAAGAAAATACTGCCA

ACAGTTTTCTAAACTACAGGATTGTGGAGCAAACTCCCAAACTTCCCATGGATGGACTCTTCCTAATCCA

AACCTATGCTGGAATGTTACAGTTAGCTAAACAGTCCTTGAAGAAGCAAGATACTCCTCAGTACAACTTA

ACGATAGAGGTGTCTGACAAAGATTTCAAGACCCTTTGTTTTGTGCAAATCAACGTTATTGATATCAATG

ATCAGATCCCCATCTTTGAAAAATCAGATTATGGAAACCTGACTCTTGCTGAAGACACAAACATTGGGTC

CACCATCTTAACCATCCAGGCCACTGATGCTGATGAGCCATTTACTGGGAGTTCTAAAATTCTGTATCAT

ATCATAAAGGGAGACAGTGAGGGACGCCTGGGGGTTGACACAGATCCCCATACCAACACCGGATATGTCA

TAATTAAAAAGCCTCTTGATTTTGAAACAGCAGCTGTTTCCAACATTGTGTTCAAAGCAGAAAATCCTGA

GCCTCTAGTGTTTGGTGTGAAGTACAATGCAAGTTCTTTTGCCAAGTTCACGCTTATTGTGACAGATGTG

AATGAAGCACCTCAATTTTCCCAACACGTATTCCAAGCGAAAGTCAGTGAGGATGTAGCTATAGGCACTA

AAGTGGGCAATGTGACTGCCAAGGATCCAGAAGGTCTGGACATAAGCTATTCACTGAGGGGAGACACAAG

AGGTTGGCTTAAAATTGACCACGTGACTGGTGAGATCTTTAGTGTGGCTCCATTGGACAGAGAAGCCGGA

AGTCCATATCGGGTACAAGTGGTGGCCACAGAAGTAGGGGGGTCTTCCTTGAGCTCTGTGTCAGAGTTCC

ACCTGATCCTTATGGATGTGAATGACAACCCTCCCAGGCTAGCCAAGGACTACACGGGCTTGTTCTTCTG

CCATCCCCTCAGTGCACCTGGAAGTCTCATTTTCGAGGCTACTGATGATGATCAGCACTTATTTCGGGGT

CCCCATTTTACATTTTCCCTCGGCAGTGGAAGCTTACAAAACGACTGGGAAGTTTCCAAAATCAATGGTA

CTCATGCCCGACTGTCTACCAGGCACACAGAGTTTGAGGAGAGGGAGTATGTCGTCTTGATCCGCATCAA

TGATGGGGGTCGGCCACCCTTGGAAGGCATTGTTTCTTTACCAGTTACATTCTGCAGTTGTGTGGAAGGA

AGTTGTTTCCGGCCAGCAGGTCACCAGACTGGGATACCCACTGTGGGCATGGCAGTTGGTATACTGCTGA

CCACCCTTCTGGTGATTGGTATAATTTTAGCAGTTGTGTTTATCCGCATAAAGAAGGATAAAGGCAAAGA

TAATGTTGAAAGTGCTCAAGCATCTGAAGTCAAACCTCTGAGAAGCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.