Human CDH15/CDH14/MCAD/Cadherin14 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin14 or CDH15 is an 814 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH15 contains an N-terminal signal peptide, a 546-aa extracellular domain (ECD) with 5 cadherin domains and 4 potential glycosylation sites, a transmembrane domain (TM), and a 188-aa highly conserved cytoplasmic tail. CDH15 is expressed in myoblasts and upregulated in myotubule-forming cells. CDH15 may control morphogenetic processes, specifically myogenesis, and provide a trigger for terminal muscle cell differentiation.  A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features, termed Mental retardation, autosomal dominant 3 (MRD3).

 

 

Gene Symbol: CDH15; CDH3; CDHM; MCAD; MRD3; CDH14; cadherin14; cadherin15

 

NCBI Gene ID: 1013

 

Uniprot Entry: P55291

 

Construct Details: Full length human CDH15 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2445 (bp)

 

Gene Insert Sequence:  

ATGGACGCCGCGTTCCTCCTCGTCCTCGGGCTGTTGGCCCAGAGCCTCTGCCTGTCTTTGGGGGTTCCTG

GATGGAGGAGGCCCACCACCCTGTACCCCTGGCGCCGGGCGCCTGCCCTGAGCCGCGTGCGGAGGGCCTG

GGTCATCCCCCCGATCAGCGTATCCGAGAACCACAAGCGTCTCCCCTACCCCCTGGTTCAGATCAAGTCG

GACAAGCAGCAGCTGGGCAGCGTCATCTACAGCATCCAGGGACCCGGCGTGGATGAGGAGCCCCGGGGCG

TCTTCTCTATCGACAAGTTCACAGGGAAGGTCTTCCTCAATGCCATGCTGGACCGCGAGAAGACTGATCG

CTTCAGGCTAAGAGCGTTTGCCCTGGACCTGGGAGGATCCACCCTGGAGGACCCCACGGACCTGGAGATT

GTAGTTGTGGATCAGAATGACAACCGGCCAGCCTTCCTGCAGGAGGCGTTCACTGGCCGCGTGCTGGAGG

GTGCAGTCCCAGGCACCTATGTGACCAGGGCAGAGGCCACAGATGCCGACGACCCCGAGACGGACAACGC

AGCGCTGCGGTTCTCCATCCTGCAGCAGGGCAGCCCCGAGCTCTTCAGCATCGACGAGCTCACAGGAGAG

ATCCGCACAGTGCAAGTGGGGCTGGACCGCGAGGTGGTCGCGGTGTACAATCTGACCCTGCAGGTGGCGG

ACATGTCTGGAGACGGCCTCACAGCCACTGCCTCAGCCATCATCACCCTTGATGACATCAATGACAATGC

CCCCGAGTTCACCAGGGATGAGTTCTTCATGGAGGCCATAGAGGCCGTCAGCGGAGTGGATGTGGGACGC

CTGGAAGTGGAGGACAGGGACCTGCCAGGCTCCCCAAACTGGGTGGCCAGGTTCACCATCCTGGAAGGCG

ACCCCGATGGGCAGTTCACCATCCGCACGGACCCCAAGACCAACGAGGGTGTTCTGTCCATTGTGAAGGC

CCTGGACTATGAGAGCTGTGAACACTACGAACTCAAAGTGTCGGTGCAGAATGAGGCCCCGCTGCAGGCG

GCTGCCCTTAGGGCTGAGCGGGGCCAGGCCAAGGTCCGCGTGCATGTGCAGGACACCAACGAGCCCCCCG

TGTTCCAGGAGAACCCACTTCGGACCAGCCTAGCAGAGGGGGCACCCCCAGGCACTCTGGTGGCCACCTT

CTCTGCCCGGGACCCTGACACAGAGCAGCTGCAGAGGCTCAGCTACTCCAAGGACTACGACCCGGAAGAC

TGGCTGCAAGTGGACGCAGCCACTGGCCGGATCCAGACCCAGCACGTGCTCAGCCCGGCGTCCCCCTTCC

TCAAGGGCGGCTGGTACAGAGCCATCGTCCTGGCCCAGGATGACGCCTCCCAGCCCCGCACCGCCACCGG

CACCCTGTCCATCGAGATCCTGGAGGTGAACGACCATGCACCTGTGCTGGCCCCGCCGCCGCCGGGCAGC

CTGTGCAGCGAGCCACACCAAGGCCCAGGCCTCCTCCTGGGCGCCACGGATGAGGACCTGCCCCCCCACG

GGGCCCCCTTCCACTTCCAGCTGAGCCCCAGGCTCCCAGAGCTCGGCCGGAACTGGAGCCTCAGCCAGGT

CAACGTGAGCCACGCGCGCCTGCGGCCGCGACACCAGGTCCCCGAAGGCCTGCACCGCCTCAGCCTGCTG

CTCCGGGACTCGGGGCAGCCGCCCCAGCAGCGCGAGCAGCCTCTGAACGTGACCGTGTGCCGCTGCGGCA

AGGACGGCGTCTGCCTGCCGGGGGCCGCAGCGCTGCTGGCGGGGGGCACAGGCCTCAGCCTGGGCGCACT

GGTCATCGTGCTGGCCAGCGCCCTCCTGCTGCTGGTGCTGGTCCTGCTCGTGGCACTCCGGGCGCGGTTC

TGGAAGCAGTCTCGGGGCAAGGGGCTGCTGCACGGCCCCCAGGACGACCTTCGAGACAATGTCCTCAACT

ACGATGAGCAAGGAGGCGGGGAGGAGGACCAGGACGCCTACGACATCAGCCAGCTGCGTCACCCGACAGC

GCTGAGCCTGCCTCTGGGACCGCCGCCACTTCGCAGAGATGCCCCGCAGGGCCGCCTGCACCCCCAGCCA

CCCCGAGTGCTGCCCACCAGCCCCCTGGACATCGCCGACTTCATCAATGATGGCTTGGAGGCTGCAGATA

GTGACCCCAGTGTGCCGCCTTACGACACAGCCCTCATCTATGACTACGAGGGTGACGGCTCGGTGGCGGG

GACGCTGAGCTCCATCCTGTCCAGCCAGGGCGATGAGGACCAGGACTACGACTACCTCAGAGACTGGGGG

CCCCGCTTCGCCCGGCTGGCAGACATGTATGGGCACCCGTGCGGGTTGGAGTACGGGGCCAGATGGGACC

ACCAGGCCAGGGAGGGTCTTTCTCCTGGGGCACTGCTACCCAGACACAGAGGCCGGACAGCCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.