Human CDH12/CDHB/Cadherin12 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin12 or CDH12 is a 794 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH12 contains an N-terminal signal peptide, a 555-aa extracellular domain (ECD) with 5 cadherin domains and 4 potential glycosylation sites, a transmembrane domain (TM), and a 157-aa highly conserved cytoplasmic tail.  CDH12 is a type II cadherin that may play an important role in cell differentiation and morphogenesis. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. CDH12 is expressed specifically in the brain and its temporal pattern of expression suggests a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.

 

 

Gene Symbol: CDH12; CDHB; Cadherin12; Cadherin-12; BR-cadherin; N-cadherin2

 

NCBI Gene ID: 1010

 

Uniprot Entry: P55289

 

Construct Details: Full length human CDH12 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2385 (bp)

 

Gene Insert Sequence:  

ATGCTTACAAGGAACTGTTTATCCCTGCTTCTCTGGGTTCTGTTTGATGGAGGTCTCCTAACACCACTAC

AACCACAGCCACAGCAGACTTTAGCCACAGAGCCAAGAGAAAATGTTATCCATCTGCCAGGACAACGGTC

ACATTTCCAACGTGTTAAACGTGGCTGGGTATGGAATCAATTTTTTGTGCTGGAAGAATACGTGGGCTCC

GAGCCTCAGTATGTGGGAAAGCTCCATTCCGACTTAGACAAGGGAGAGGGCACTGTGAAATACACCCTCT

CAGGAGATGGCGCTGGCACCGTTTTTACCATTGATGAAACCACAGGGGACATTCATGCAATAAGGAGCCT

AGATAGAGAAGAGAAACCTTTCTACACTCTTCGTGCTCAGGCTGTGGACATAGAAACCAGAAAGCCCCTG

GAGCCTGAATCAGAATTCATCATCAAAGTGCAGGATATTAATGATAATGAGCCAAAGTTTTTGGATGGAC

CTTATGTTGCTACTGTTCCAGAAATGTCTCCTGTGGGTGCATATGTACTCCAGGTCAAGGCCACAGATGC

AGATGACCCGACCTATGGAAACAGTGCCAGAGTCGTTTACAGCATTCTTCAGGGACAACCTTATTTCTCT

ATTGATCCCAAGACAGGTGTTATTAGAACAGCTTTGCCAAACATGGACAGAGAAGTCAAAGAACAATATC

AAGTACTCATCCAAGCCAAGGATATGGGAGGACAGCTTGGAGGATTAGCCGGAACAACAATAGTCAACAT

CACTCTCACCGATGTCAATGACAATCCACCTCGATTCCCCAAAAGCATCTTCCACTTGAAAGTTCCTGAG

TCTTCCCCTATTGGTTCAGCTATTGGAAGAATAAGAGCTGTGGATCCTGATTTTGGACAAAATGCAGAAA

TTGAATACAATATTGTTCCAGGAGATGGGGGAAATTTGTTTGACATCGTCACAGATGAGGATACACAAGA

GGGAGTCATCAAATTGAAAAAGCCTTTAGATTTTGAAACAAAGAAGGCATACACTTTCAAAGTTGAGGCT

TCCAACCTTCACCTTGACCACCGGTTTCACTCGGCGGGCCCTTTCAAAGACACAGCTACGGTGAAGATCA

GCGTGCTGGACGTAGATGAGCCACCGGTTTTCAGCAAGCCGCTCTACACCATGGAGGTTTATGAAGACAC

TCCGGTAGGGACCATCATTGGCGCTGTCACTGCTCAAGACCTGGATGTAGGCAGCAGTGCTGTTAGGTAC

TTCATAGATTGGAAGAGTGATGGGGACAGCTACTTTACAATAGATGGAAATGAAGGAACCATCGCCACTA

ATGAATTACTAGACAGAGAAAGCACTGCGCAGTATAATTTCTCCATAATTGCGAGTAAAGTTAGTAACCC

TTTATTGACCAGCAAAGTCAATATACTGATTAATGTCTTAGATGTAAATGAATTTCCTCCAGAAATATCT

GTGCCATATGAGACAGCCGTGTGTGAAAATGCCAAGCCAGGACAGATAATTCAGATAGTCAGTGCTGCAG

ACCGAGATCTTTCACCTGCTGGGCAACAATTCTCCTTTAGATTATCACCTGAGGCTGCTATCAAACCAAA

TTTTACAGTTCGTGACTTCAGAAACAACACAGCGGGGATTGAAACCCGAAGAAATGGATACAGCCGCAGG

CAGCAAGAGTTGTATTTCCTCCCTGTTGTAATAGAAGACAGCAGCTACCCTGTCCAGAGCAGCACAAACA

CAATGACTATTCGAGTCTGTAGATGTGACTCTGATGGCACCATCCTGTCTTGTAATGTGGAAGCAATTTT

TCTACCTGTAGGACTTAGCACTGGGGCGTTGATTGCAATTCTACTATGCATTGTTATACTCTTAGCCATA

GTTGTACTGTATGTAGCACTGCGAAGGCAGAAGAAAAAAGACACCCTGATGACCTCTAAAGAAGACATCA

GAGACAACGTCATCCATTACGATGATGAAGGAGGTGGGGAGGAAGATACCCAGGCTTTCGACATCGGGGC

TCTGAGAAACCCAAAAGTGATTGAGGAGAACAAAATTCGCAGGGATATAAAACCAGACTCTCTCTGTTTA

CCTCGTCAGAGACCACCCATGGAAGATAACACAGACATAAGGGATTTCATTCATCAAAGGCTACAGGAAA

ATGATGTGGATCCAACTGCCCCACCATACGATTCACTGGCCACATATGCCTACGAAGGGAGTGGGTCCGT

GGCAGAGTCCCTCAGCTCTATAGACTCTCTCACCACAGAAGCCGACCAGGACTATGACTATCTGACAGAC

TGGGGACCCCGCTTTAAAGTCTTGGCAGACATGTTTGGCGAAGAAGAGAGTTATAACCCTGATAAAGTCA

CTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.