Human CDH10/Cadherin10 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin10 or CDH10 is a 788 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH10 contains an N-terminal signal peptide, a 559-aa extracellular domain (ECD) with 5 cadherin domains and 4 potential glycosylation sites, a transmembrane domain (TM), and a 154-aa highly conserved cytoplasmic tail.  CDH10 is a type II cadherin that may play an important role in cell differentiation and morphogenesis. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. CDH10 is predominantly expressed in brain and may be involved in synaptic adhesions, axon outgrowth and guidance. Mutations in the CDH10 gene may be associated with lung squamous cell carcinoma and colorectal cancer in human patients.

 

 

Gene Symbol: CDH10; Cadherin10; Cadherin-10

 

NCBI Gene ID: 1008

 

Uniprot Entry: Q9Y6N8

 

Construct Details: Full length human CDH10 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2367 (bp)

 

Gene Insert Sequence:  

ATGACAATACATCAATTTTTGCTACTGTTTCTATTCTGGGTATGCCTGCCACATTTCTGCTCTCCAGAAA

TAATGTTCAGAAGGACGCCTGTGCCACAGCAAAGAATTTTAAGTTCACGTGTACCAAGGAGTGATGGCAA

AATTCTCCATCGTCAAAAACGTGGTTGGATGTGGAATCAATTTTTCTTACTTGAAGAATATACAGGATCT

GATTATCAGTACGTAGGCAAGCTACATTCAGACCAAGATAAAGGAGATGGATCACTCAAATATATCTTAT

CTGGAGATGGAGCTGGTACTCTTTTTATTATTGATGAAAAAACAGGTGATATTCATGCCACAAGGCGAAT

TGATAGGGAGGAAAAGGCCTTTTATACTCTACGCGCACAAGCTATTAACAGAAGAACTCTGAGGCCAGTA

GAGCCAGAGTCAGAGTTTGTGATCAAAATTCATGATATCAATGACAATGAGCCAACGTTCCCAGAAGAAA

TCTATACAGCTAGTGTTCCCGAAATGTCTGTTGTAGGTACTTCTGTGGTGCAAGTCACAGCTACAGATGC

CGATGACCCTTCATATGGGAACAGCGCCAGAGTCATTTACAGCATACTTCAAGGGCAGCCCTATTTCTCT

GTGGAGCCTGAAACAGGTATCATCAGGACTGCTTTACCGAACATGAACAGAGAAAACAGAGAGCAATACC

AAGTGGTCATCCAGGCCAAAGACATGGGCGGCCAGATGGGAGGCTTATCGGGGACAACCACTGTGAACAT

CACGCTGACAGATGTCAATGACAACCCACCACGTTTCCCCCAGAACACTATTCATCTTCGAGTTCTTGAA

TCCTCCCCAGTTGGCACAGCCATTGGAAGTGTCAAAGCAACTGATGCTGACACTGGGAAAAATGCTGAAG

TAGAATACCGAATTATTGATGGTGACGGTACTGATATGTTTGACATCGTGACTGAGAAGGACACACAGGA

AGGCATCATCACTGTGAAAAAGCCACTCGACTATGAGAGCCGAAGACTTTATACTCTGAAAGTCGAAGCA

GAAAACACCCATGTAGATCCCCGTTTTTATTACCTAGGACCATTTAAAGATACTACCATAGTGAAAATCT

CTATAGAAGATGTGGATGAACCTCCTGTTTTTAGTAGGTCCTCCTATCTGTTTGAAGTTCATGAAGATAT

TGAAGTGGGCACAATCATTGGTACTGTAATGGCAAGGGACCCAGATTCTATTTCCAGCCCCATTAGATTT

TCCTTGGATCGCCATACTGACCTTGACAGAATCTTTAACATTCATTCAGGAAATGGATCTCTTTATACAT

CAAAACCTCTTGACCGTGAACTATCTCAGTGGCATAATCTTACTGTTATTGCTGCTGAAATCAACAATCC

CAAAGAGACAACACGCGTGGCTGTTTTTGTGAGAATTTTGGATGTTAATGACAATGCCCCACAGTTTGCT

GTGTTCTATGACACTTTTGTATGTGAAAATGCCAGACCAGGGCAGCTAATACAGACTATAAGTGCAGTAG

ACAAAGATGACCCTTTAGGTGGACAGAAATTTTTTTTCAGTTTAGCTGCTGTCAATCCAAACTTCACAGT

ACAGGATAATGAAGATAATACTGCCAGAATCTTAACCAGAAAAAATGGATTCAATAGACATGAAATCAGT

ACCTATCTCTTGCCTGTGGTGATATCAGACAATGATTACCCAATTCAGAGCAGCACAGGCACACTGACCA

TTCGAGTGTGTGCTTGTGACAGCCAAGGCAACATGCAATCCTGCAGTGCTGAAGCCCTGCTCCTCCCTGC

CGGCCTCAGCACTGGGGCCTTGATCGCCATCCTCCTCTGCATCATCATTCTACTGGTTATAGTAGTACTG

TTTGCAGCTCTGAAAAGACAGCGAAAAAAAGAGCCTCTGATCTTGTCAAAAGAAGATATCAGAGACAACA

TTGTGAGCTATAACGATGAGGGTGGTGGAGAGGAGGACACCCAGGCCTTTGATATCGGCACCCTGAGGAA

TCCTGCAGCCATTGAGGAAAAAAAGCTCCGGCGAGATATTATTCCAGAAACGTTATTTATTCCTCGGAGG

ACTCCTACAGCTCCAGATAACACGGACGTCCGGGATTTCATTAATGAAAGGCTAAAAGAGCATGATCTTG

ACCCCACCGCACCCCCCTACGACTCACTTGCAACCTATGCCTATGAAGGAAATGATTCCATTGCTGAATC

TCTGAGTTCATTAGAATCAGGTACTACTGAAGGAGACCAAAACTACGATTACCTCCGAGAATGGGGCCCT

CGGTTTAATAAGCTAGCAGAAATGTATGGTGGTGGGGAAAGTGACAAAGACTCTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.