Human CDH5//CD144/7B4/Cadherin5 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin5, CDH5 or CD144 is a 784 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH5/CD144 contains an N-terminal signal peptide, a 552-aa extracellular domain (ECD) with 5 cadherin domains and 7 potential glycosylation sites, a transmembrane domain (TM), and a 164-aa highly conserved cytoplasmic tail.  CDH5 is found at cell-cell and cell-matrix boundaries. CDH5 can be phosphorylated on tyrosine residues by KDR/VEGFR-2 and dephosphorylated by PTPRB.  CDH5 may play an important role in endothelial junction assembly and maintenance. through control of the cohesion and organization of the intercellular junctions. CDH5 associates with alpha-catenin forming a link to the cytoskeleton. CDH5 also acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. CDH5 is required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. The CDH5 gene is located in a gene cluster in a region of chromosome 16 involved in loss of heterozygosity events in breast and prostate cancer.

 

 

Gene Symbol: CDH5; 7B4; CD144; Cadherin5; VE-cadherin

 

NCBI Gene ID: 1003

 

Uniprot Entry: P33151

 

Construct Details: Full length human CDH5/CD144 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2355 (bp)

 

Gene Insert Sequence:  

ATGCAGAGGCTCATGATGCTCCTCGCCACATCGGGCGCCTGCCTGGGCCTGCTGGCAGTGGCAGCAGTGG

CAGCAGCAGGTGCTAACCCTGCCCAACGGGACACCCACAGCCTGCTGCCCACCCACCGGCGCCAAAAGAG

AGATTGGATTTGGAACCAGATGCACATTGATGAAGAGAAAAACACCTCACTTCCCCATCATGTAGGCAAG

ATCAAGTCAAGCGTGAGTCGCAAGAATGCCAAGTACCTGCTCAAAGGAGAATATGTGGGCAAGGTCTTCC

GGGTCGATGCAGAGACAGGAGACGTGTTCGCCATTGAGAGGCTGGACCGGGAGAATATCTCAGAGTACCA

CCTCACTGCTGTCATTGTGGACAAGGACACTGGCGAAAACCTGGAGACTCCTTCCAGCTTCACCATCAAA

GTTCATGACGTGAACGACAACTGGCCTGTGTTCACGCATCGGTTGTTCAATGCGTCCGTGCCTGAGTCGT

CGGCTGTGGGGACCTCAGTCATCTCTGTGACAGCAGTGGATGCAGACGACCCCACTGTGGGAGACCACGC

CTCTGTCATGTACCAAATCCTGAAGGGGAAAGAGTATTTTGCCATCGATAATTCTGGACGTATTATCACA

ATAACGAAAAGCTTGGACCGAGAGAAGCAGGCCAGGTATGAGATCGTGGTGGAAGCGCGAGATGCCCAGG

GCCTCCGGGGGGACTCGGGCACGGCCACCGTGCTGGTCACTCTGCAAGACATCAATGACAACTTCCCCTT

CTTCACCCAGACCAAGTACACATTTGTCGTGCCTGAAGACACCCGTGTGGGCACCTCTGTGGGCTCTCTG

TTTGTTGAGGACCCAGATGAGCCCCAGAACCGGATGACCAAGTACAGCATCTTGCGGGGCGACTACCAGG

ACGCTTTCACCATTGAGACAAACCCCGCCCACAACGAGGGCATCATCAAGCCCATGAAGCCTCTGGATTA

TGAATACATCCAGCAATACAGCTTCATCGTCGAGGCCACAGACCCCACCATCGACCTCCGATACATGAGC

CCTCCCGCGGGAAACAGAGCCCAGGTCATTATCAACATCACAGATGTGGACGAGCCCCCCATTTTCCAGC

AGCCTTTCTACCACTTCCAGCTGAAGGAAAACCAGAAGAAGCCTCTGATTGGCACAGTGCTGGCCATGGA

CCCTGATGCGGCTAGGCATAGCATTGGATACTCCATCCGCAGGACCAGTGACAAGGGCCAGTTCTTCCGA

GTCACAAAAAAGGGGGACATTTACAATGAGAAAGAACTGGACAGAGAAGTCTACCCCTGGTATAACCTGA

CTGTGGAGGCCAAAGAACTGGATTCCACTGGAACCCCCACAGGAAAAGAATCCATTGTGCAAGTCCACAT

TGAAGTTTTGGATGAGAATGACAATGCCCCGGAGTTTGCCAAGCCCTACCAGCCCAAAGTGTGTGAGAAC

GCTGTCCATGGCCAGCTGGTCCTGCAGATCTCCGCAATAGACAAGGACATAACACCACGAAACGTGAAGT

TCAAATTCATCTTGAATACTGAGAACAACTTTACCCTCACGGATAATCACGATAACACGGCCAACATCAC

AGTCAAGTATGGGCAGTTTGACCGGGAGCATACCAAGGTCCACTTCCTACCCGTGGTCATCTCAGACAAT

GGGATGCCAAGTCGCACGGGCACCAGCACGCTGACCGTGGCCGTGTGCAAGTGCAACGAGCAGGGCGAGT

TCACCTTCTGCGAGGATATGGCCGCCCAGGTGGGCGTGAGCATCCAGGCAGTGGTAGCCATCTTACTCTG

CATCCTCACCATCACAGTGATCACCCTGCTCATCTTCCTGCGGCGGCGGCTCCGGAAGCAGGCCCGCGCG

CACGGCAAGAGCGTGCCGGAGATCCACGAGCAGCTGGTCACCTACGACGAGGAGGGCGGCGGCGAGATGG

ACACCACCAGCTACGATGTGTCGGTGCTCAACTCGGTGCGCCGCGGCGGGGCCAAGCCCCCGCGGCCCGC

GCTGGACGCCCGGCCTTCCCTCTATGCGCAGGTGCAGAAGCCACCGAGGCACGCGCCTGGGGCACACGGA

GGGCCCGGGGAGATGGCAGCCATGATCGAGGTGAAGAAGGACGAGGCGGACCACGACGGCGACGGCCCCC

CCTACGACACGCTGCACATCTACGGCTACGAGGGCTCCGAGTCCATAGCCGAGTCCCTCAGCTCCCTGGG

CACCGACTCATCCGACTCTGACGTGGATTACGACTTCCTTAACGACTGGGGACCCAGGTTTAAGATGCTG

GCTGAGCTGTACGGCTCGGACCCCCGGGAGGAGCTGCTGTATTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.