Human CDH4/CAD4/RCAD/Cadherin4 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells.  Cadherins thus contribute to the sorting of heterogeneous cell types.  Cadherin4 or CDH4 is a 916 amino acid (aa) single pass type I transmembrane protein of the cadherin superfamily.  CDH4 contains an N-terminal signal peptide, a 565-aa extracellular domain (ECD) with 5 cadherin domains and 6 potential glycosylation sites, a transmembrane domain (TM), and a 160-aa highly conserved cytoplasmic tail.  CDH4 may play an important role during brain segmentation and neuronal outgrowth as well as in kidney and muscle development. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in co-transfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. 

 

 

Gene Symbol: CDH4; CAD4; RCAD; R-CAD; R-cadherin; Cadherin4

 

NCBI Gene ID: 1002

 

Uniprot Entry: P55283

 

Construct Details: Full length human CDH4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2751 (bp)

 

Gene Insert Sequence:  

ATGACCGCGGGCGCCGGCGTGCTCCTTCTGCTGCTCTCGCTCTCCGGCGCGCTCCGGGCCCATAATGAGG

ATCTTACAACTAGAGAGACCTGCAAGGCTGGGTTCTCTGAAGATGATTACACGGCATTAATCTCCCAAAA

TATTCTAGAAGGGGAAAAGCTACTTCAAGTCAAGTTCAGCAGCTGTGTGGGGACCAAGGGGACACAATAT

GAGACCAACAGCATGGACTTCAAAGTTGGGGCAGATGGGACAGTCTTCGCCACCCGGGAGCTGCAGGTCC

CCTCCGAGCAGGTGGCGTTCACGGTGACTGCATGGGACAGCCAGACAGCAGAGAAATGGGACGCCGTGGT

GCGGTTGCTGGTGGCCCAGACCTCGTCCCCGCACTCTGGACACAAGCCGCAGAAAGGAAAGAAGGTCGTG

GCTCTGGACCCCTCTCCGCCTCCGAAGGACACCCTGCTGCCGTGGCCCCAGCACCAGAACGCCAACGGGC

TGAGGCGGCGCAAACGGGACTGGGTCATCCCGCCCATCAACGTGCCCGAGAACTCGCGCGGGCCCTTCCC

GCAGCAGCTCGTGAGGATCCGGTCCGACAAAGACAATGACATCCCCATCCGGTACAGCATCACGGGAGTG

GGCGCCGACCAGCCCCCCATGGAGGTCTTCAGCATTGACTCCATGTCCGGCCGGATGTACGTCACAAGGC

CCATGGACCGGGAGGAGCACGCCTCTTACCACCTCCGAGCCCACGCTGTGGACATGAATGGCAACAAGGT

GGAGAACCCCATCGACCTGTACATCTACGTCATCGACATGAATGACAACCGCCCTGAGTTCATCAACCAG

GTCTACAACGGCTCCGTGGACGAGGGCTCCAAGCCAGGCACCTACGTGATGACCGTCACGGCCAACGATG

CTGACGACAGCACCACGGCCAACGGGATGGTGCGGTACCGGATCGTGACCCAGACCCCACAGAGCCCGTC

CCAGAATATGTTCACCATCAACAGCGAGACTGGAGATATCGTCACAGTGGCGGCTGGCCTGGACCGAGAG

AAAGTTCAGCAGTACACAGTCATCGTTCAGGCCACAGATATGGAAGGAAATCTCAACTATGGCCTCTCAA

ACACAGCCACAGCCATCATCACGGTGACAGATGTGAATGACAACCCGCCAGAATTTACCGCCAGCACGTT

TGCAGGGGAGGTCCCCGAAAACCGCGTGGAGACCGTGGTCGCAAACCTCACGGTGATGGACCGAGATCAG

CCCCACTCTCCAAACTGGAATGCCGTTTACCGCATCATCAGTGGGGATCCATCCGGGCACTTCAGCGTCC

GCACAGACCCCGTAACCAACGAGGGCATGGTCACCGTGGTGAAGGCAGTCGACTACGAGCTCAACAGAGC

TTTCATGCTGACAGTGATGGTGTCCAACCAGGCGCCCCTGGCCAGCGGAATCCAGATGTCCTTCCAGTCC

ACGGCAGGGGTGACCATCTCCATCATGGACATCAACGAGGCTCCCTACTTCCCCTCAAACCACAAGCTGA

TCCGCCTGGAGGAGGGCGTGCCCCCCGGCACCGTGCTGACCACGTTTTCAGCTGTGGACCCTGACCGGTT

CATGCAGCAGGCTGTGAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCTGCACATCAATGCCACCAAC

GGCCAGATCACCACGGCGGCAGTGCTGGACCGTGAGTCCCTCTACACCAAAAACAACGTCTACGAGGCCA

CCTTCCTGGCAGCTGACAATGGGATACCCCCGGCCAGCGGCACCGGGACCCTCCAGATCTATCTCATTGA

CATCAACGACAACGCCCCTGAGCTGCTGCCCAAGGAGGCGCAGATCTGCGAGAAGCCCAACCTGAACGCC

ATCAACATCACGGCGGCCGACGCTGACGTCGACCCCAACATCGGCCCCTACGTCTTCGAGCTGCCCTTTG

TCCCGGCGGCCGTGCGGAAGAACTGGACCATCACCCGCCTGAACGGTGACTATGCCCAACTCAGCTTGCG

CATCCTGTACCTGGAGGCCGGGATGTATGACGTCCCCATCATCGTCACAGACTCTGGAAACCCTCCCCTG

TCCAACACGTCCATCATCAAAGTCAAGGTGTGCCCATGTGATGACAACGGGGACTGCACCACCATTGGCG

CAGTGGCAGCGGCTGGTCTGGGCACCGGTGCCATCGTGGCCATCCTCATCTGCATCCTCATCCTGCTGAC

CATGGTCCTGCTGTTTGTCATGTGGATGAAGCGGCGAGAGAAGGAGCGCCACACGAAGCAGCTGCTCATT

GACCCCGAGGACGACGTCCGCGACAACATCCTCAAGTATGACGAGGAAGGCGGTGGCGAGGAGGACCAGG

ACTACGACCTCAGCCAGCTGCAGCAGCCGGAAGCCATGGGGCACGTGCCAAGCAAAGCCCCTGGCGTGCG

TCGCGTGGATGAGCGGCCGGTGGGCGCTGAGCCCCAGTACCCGATCAGGCCCATGGTGCCGCACCCAGGC

GACATCGGTGACTTCATCAATGAGGGACTCCGCGCTGCTGACAACGACCCCACGGCACCCCCCTATGACT

CCCTGCTGGTCTTCGACTACGAGGGGAGCGGCTCCACCGCAGGCTCCGTCAGCTCCCTGAACTCATCCAG

TTCCGGGGACCAAGACTACGATTACCTCAACGACTGGGGGCCCAGATTCAAGAAGCTGGCGGACATGTAT

GGAGGTGGTGAAGAGGATTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.