Human EVI2B/CD361/EVDB Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

EVI2B (ecotropic viral integration site 2B protein homolog) or CD361 is a 448 amino acid (aa) single pass type I transmembrane protein.  EVI2B is proline-rich and contains an N-terminal signal peptide, an 181 aa extracellular domain (ECD) with 4 potential glycosylation sites, a transmembrane domain (TM), and a 222 aa cytoplasmic hydrophilic tail.  EVI2B and EVI2A were the genes originally identified between the 2 translocation breakpoints that interrupt the NF1 gene in patients with neurofibromatosis I. EVI2B is expressed in bone marrow, peripheral blood mononuclear cells, fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell lines.

 

 

Gene Symbol: EVI2B; EVDB; CD361; D17S376

 

NCBI Gene ID: 2124

 

Uniprot Entry: P34910

 

Construct Details: Full length human EVI2B gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1347 (bp)

 

Gene Insert Sequence:  

ATGGATCCCAAATATTTCATCTTAATTTTGTTTTGTGGACACCTGAACAATACATTTTTTTCAAAGACAG

AGACAATTACAACAGAGAAGCAGTCACAGCCTACCTTATTCACATCATCAATGTCACAGGTATTGGCTAA

TTCTCAAAACACAACAGGGAATCCTTTGGGTCAACCAACACAATTCAGCGACACTTTTTCTGGACAATCA

ATATCACCTGCCAAAGTCACTGCTGGACAACCAACACCAGCTGTCTATACCTCTTCTGAAAAACCAGAAG

CACATACTTCTGCTGGACAACCACTTGCCTACAACACCAAACAACCAACACCAATAGCCAACACCTCCTC

CCAGCAAGCCGTGTTCACCTCTGCCAGACAACTACCATCTGCCCGTACTTCTACCACACAACCACCAAAG

TCATTTGTCTATACTTTTACTCAACAATCATCATCTGTCCAGATCCCTTCTAGAAAACAAATAACTGTTC

ATAATCCATCCACACAACCAACATCAACTGTCAAAAATTCACCTAGGAGTACACCAGGATTTATCTTAGA

TACTACCAGTAACAAACAAACCCCACAAAAAAACAATTATAATTCAATAGCTGCCATACTAATTGGTGTA

CTTCTGACTTCTATGTTGGTAGCTATAATCATCATTGTACTTTGGAAATGCTTAAGGAAACCAGTTTTAA

ATGATCAAAATTGGGCAGGTAGATCTCCATTTGCTGATGGAGAAACCCCTGACATTTGTATGGATAACAT

CAGAGAAAATGAAATATCCACAAAACGTACATCAATCATTTCACTTACACCCTGGAAACCAAGCAAAAGC

ACACTTTTAGCAGATGACTTAGAAATTAAGTTGTTTGAATCAAGTGAAAACATTGAAGACTCCAACAACC

CCAAAACAGAGAAAATAAAAGATCAAGTAAATGGTACATCAGAAGATAGTGCTGATGGTTCAACAGTTGG

AACTGCTGTTTCTTCTTCAGATGATGCAGATCTGCCTCCACCACCTCCCCTTCTGGATTTGGAAGGACAG

GAAAGTAACCAATCTGACAAACCCACAATGACAATTGTATCTCCTCTTCCAAATGATTCTACTAGTCTCC

CTCCATCTCTGGACTGTCTCAATCAAGACTGTGGAGATCATAAATCTGAGATAATACAATCATTTCCACC

GCTTGACTCACTTAACTTGCCCCTGCCACCAGTAGATTTTATGAAAAACCAAGAAGATTCCAACCTTGAG

ATCCAGTGTCAGGAGTTCTCTATTCCTCCCAACTCTGATCAAGATCTTAATGAATCCCTGCCACCTCCAC

CTGCAGAACTGTTATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.