Human SDC3/syndecan3/SYND3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

SDC3 (syndecan-3) is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. SDC3 is a 442 amino acid (aa) single pass type I transmembrane protein. SDC3 contains an N-terminal signal peptide, a Ser/Thr-rich (mucin-like) 387-aa extracellular domain (ECD) with 6 potential glycosylation sites, a transmembrane domain (TM), and a short 34-aa cytoplasmic tail.  SDC3 is expressed in the nervous system, the adrenal gland, and the spleen.  SDC3 may act as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. SDC3 may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of the SDC3 gene are associated with obesity. 

 

 

Gene Symbol: SDC3; SDCN; SYND3; Syndecan3; KIAA0468

 

NCBI Gene ID: 9672

 

Uniprot Entry: O75056

 

Construct Details: Full length human SDC3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1329 (bp)

 

Gene Insert Sequence:  

ATGAAGCCGGGGCCGCCGCACCGTGCCGGGGCCGCCCACGGGGCCGGCGCCGGGGCCGGGGCCGCGGCCG

GGCCCGGGGCCCGCGGGCTGCTCCTGCCACCGCTGCTGCTGCTGCTGCTGGCGGGGCGCGCCGCGGGGGC

CCAGCGCTGGCGCAGTGAGAACTTCGAGAGACCCGTGGACCTGGAGGGCTCTGGGGATGATGACTCCTTT

CCCGATGATGAACTGGATGACCTCTACTCGGGGTCGGGCTCGGGCTACTTCGAGCAGGAGTCGGGCATTG

AGACAGCCATGCGCTTCAGCCCAGATGTAGCCCTGGCGGTGTCCACCACACCTGCGGTGCTGCCCACCAC

GAACATCCAGCCTGTGGGCACACCATTTGAAGAGCTCCCCTCTGAGCGCCCCACCCTGGAGCCAGCCACC

AGCCCCCTGGTGGTGACAGAAGTCCCGGAAGAGCCCAGCCAGAGAGCCACCACCGTCTCCACTACCATGG

CTACCACTGCTGCCACAAGCACAGGGGACCCGACTGTGGCCACAGTGCCTGCCACAGTGGCCACCGCCAC

CCCCAGCACCCCTGCAGCACCCCCTTTTACGGCCACCACTGCTGTTATAAGGACCACTGGCGTACGGAGG

CTTCTGCCTCTCCCACTGACCACAGTGGCTACGGCACGGGCCACTACCCCCGAGGCGCCCTCCCCGCCCA

CCACGGCGGCTGTCTTGGACACCGAGGCCCCAACACCCAGGCTGGTCAGCACAGCTACCTCCCGGCCAAG

AGCCCTTCCCAGGCCGGCCACCACCCAGGAGCCTGACATCCCTGAGAGGAGCACCCTGCCCCTGGGGACC

ACTGCCCCTGGACCCACAGAGGTGGCTCAGACCCCAACTCCAGAGACCTTCCTGACCACAATCCGGGATG

AGCCAGAGGTTCCGGTGAGTGGGGGGCCCAGTGGAGACTTCGAGCTGCCAGAAGAAGAGACCACACAACC

AGACACAGCCAATGAGGTGGTAGCTGTGGGAGGGGCTGCGGCCAAGGCATCATCTCCACCTGGGACACTG

CCCAAGGGTGCCCGCCCGGGCCCTGGCCTCCTGGACAATGCCATCGACTCGGGCAGCTCAGCTGCTCAGC

TGCCTCAGAAGAGTATCCTGGAGCGGAAGGAGGTGCTCGTAGCTGTGATTGTGGGCGGGGTGGTGGGCGC

CCTCTTTGCTGCCTTCTTGGTCACACTGCTCATCTATCGTATGAAGAAAAAGGATGAGGGCAGCTACACG

CTGGAGGAACCCAAGCAGGCGAGCGTCACATACCAGAAGCCTGACAAGCAGGAGGAGTTCTATGCCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.