Human Nectin3/CD113/PRR3/PVRL3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

Nectin3 or CD113 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF).  Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin3 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected with NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1 and with IGSF4. Nectin3 also interacts with with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. Nectin3 may be involved in the formation of cell-cell junctions, including adherens junctions and synapses. Nectin3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Nectin3 plays a role in the morphology of the ciliary body and in ocular development involving the ciliary body. Mutations in the Nectin3 gene are believed to result in congenital ocular defects. 

 

 

Gene Symbol: Nectin3; CD113; PPR3; PRR3; PVRL3; PVRR3; CDW113; NECTIN-3

 

NCBI Gene ID: 25945

 

Uniprot Entry: Q9NQS3

 

Construct Details: Full length human NECTIN3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. 

 

Vector Type: pLTC or pLTC-Marker (lentiviral expression vector containing a heterologous CMV promoter -/+ a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1650 (bp)

 

Gene Insert Sequence:  

ATGGCGCGGACCCTGCGGCCGTCCCCGCTGTGTCCTGGAGGCGGCAAAGCACAACTTTCCTCCGCTTCTC

TCCTCGGAGCCGGGCTCCTGCTGCAGCCCCCGACGCCACCTCCGCTGCTGCTGCTGCTCTTCCCGCTGCT

GCTCTTCTCCAGGCTCTGTGGTGCCTTAGCTGGACCAATTATTGTGGAGCCACATGTCACAGCAGTATGG

GGAAAGAATGTTTCATTAAAGTGTTTAATTGAAGTAAATGAAACCATAACACAGATTTCATGGGAGAAGA

TACATGGCAAAAGTTCACAGACTGTTGCAGTTCACCATCCCCAATATGGATTCTCTGTTCAAGGAGAATA

TCAGGGAAGAGTCTTGTTTAAAAATTACTCACTTAATGATGCAACAATTACTCTGCATAACATAGGATTC

TCTGATTCTGGAAAATACATCTGCAAAGCTGTTACATTCCCGCTTGGAAATGCCCAGTCCTCTACAACTG

TAACTGTGTTAGTTGAACCCACTGTGAGCCTGATAAAAGGGCCAGATTCTTTAATTGATGGAGGAAATGA

AACAGTAGCAGCCATTTGCATCGCAGCCACTGGAAAACCCGTTGCACATATTGACTGGGAAGGTGATCTT

GGTGAAATGGAATCCACTACAACTTCTTTTCCAAATGAAACGGCAACGATTATCAGCCAGTACAAGCTAT

TTCCAACCAGATTTGCTAGAGGAAGGCGAATTACTTGTGTTGTAAAACATCCAGCCTTGGAAAAGGACAT

CCGATACTCTTTCATATTAGACATACAGTATGCTCCTGAAGTTTCGGTAACAGGATATGATGGAAATTGG

TTTGTAGGAAGAAAAGGTGTTAATCTCAAATGTAATGCTGATGCAAATCCACCACCCTTCAAATCTGTGT

GGAGCAGGTTGGATGGACAATGGCCTGATGGTTTATTGGCTTCAGACAATACTCTTCATTTTGTCCATCC

ATTGACTTTCAATTATTCTGGTGTTTATATCTGTAAAGTGACCAATTCCCTTGGTCAAAGAAGTGACCAA

AAAGTCATCTACATTTCAGATCCTCCTACTACTACCACCCTTCAGCCTACAATTCAGTGGCATCCCTCAA

CTGCTGACATCGAGGATCTAGCAACAGAACCTAAAAAATTGCCCTTCCCATTGTCAACTTTGGCAACAAT

TAAGGATGACACAATTGCCACGATCATTGCTAGTGTAGTGGGTGGGGCTCTCTTCATAGTACTTGTAAGT

GTTTTGGCTGGAATATTCTGCTATAGGAGAAGACGGACGTTTCGTGGAGACTACTTTGCCAAGAACTACA

TTCCACCATCAGATATGCAAAAAGAATCACAAATAGATGTTCTTCAACAAGATGAGCTTGATTCTTACCC

AGACAGTGTAAAAAAAGAAAACAAAAATCCAGTGAACAATCTAATACGTAAAGACTATTTAGAAGAGCCT

GAAAAAACTCAGTGGAACAATGTAGAAAATCTCAATAGGTTTGAAAGACCAATGGATTATTATGAAGATC

TAAAAATGGGAATGAAGTTTGTCAGTGATGAACATTATGATGAAAACGAAGATGACTTAGTTTCACATGT

AGATGGTTCCGTAATTTCCAGGAGGGAGTGGTATGTTTAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.