Human CD93/C1QR1/CDw93/MXRA4 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description

CD93 is a single-pass type I transmembrane protein and a member of the calcium dependent C-type lectin superfamily.  CD93 contains 1 C-type lectin domain and 5 EGF-like domains in the extracellular region. CD93 was originally identified as a myeloid cell-specific marker and was thought to be a receptor for C1q.  CD93 may be involved in intercellular adhesion and in the clearance of apoptotic cells. CD93 is highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils but not expressed in cells of lymphoid origin. CD93 interacts with HCV core protein.  CD93 also binds C1QBP; the association may represent a cell surface C1q receptor. The intracellular cytoplasmic tail of CD93 may interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.  

 

 

Gene Symbol: CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1

 

NCBI Gene ID: 22918

 

Uniprot Entry: O9NPY3

 

Construct Details: The recombinant human CD93-Fc fusion is expressed as a 785 amino acid protein consisting of Ala24 - Lys580 region of CD93 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

  

Source: Human cells stably expressing human CD93-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ADTEAVVCVG TACYTAHSGK LSAAEAQNHC NQNGGNLATV KSKEEAQHVQ RVLAQLLRRE 

        70         80         90        100        110        120 

AALTARMSKF WIGLQREKGK CLDPSLPLKG FSWVGGGEDT PYSNWHKELR NSCISKRCVS 

       130        140        150        160        170        180 

LLLDLSQPLL PSRLPKWSEG PCGSPGSPGS NIEGFVCKFS FKGMCRPLAL GGPGQVTYTT 

       190        200        210        220        230        240 

PFQTTSSSLE AVPFASAANV ACGEGDKDET QSHYFLCKEK APDVFDWGSS GPLCVSPKYG 

       250        260        270        280        290        300 

CNFNNGGCHQ DCFEGGDGSF LCGCRPGFRL LDDLVTCASR NPCSSSPCRG GATCVLGPHG 

       310        320        330        340        350        360 

KNYTCRCPQG YQLDSSQLDC VDVDECQDSP CAQECVNTPG GFRCECWVGY EPGGPGEGAC 

       370        380        390        400        410        420 

QDVDECALGR SPCAQGCTNT DGSFHCSCEE GYVLAGEDGT QCQDVDECVG PGGPLCDSLC 

       430        440        450        460        470        480 

FNTQGSFHCG CLPGWVLAPN GVSCTMGPVS LGPPSGPPDE EDKGEKEGST VPRAATASPT 

       490        500        510        520        530        540 

RGPEGTPKAT PTTSRPSLSS DAPITSAPLK MLAPSGSPGV WREPSIHHAT AASGPQEPAG 

       550        560        570        580        590        600 

GDSSVATQNN DGTDGQKVDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       610        620        630        640        650        660 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 

       670        680        690        700        710        720 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 

       730        740        750        760        770        780 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

 

LSPGK 

  

M.W.: Calculated molecular mass (kDa): 83.8; Estimated by SDS-PAGE under reducing condition (kDa): 3 major bands 100-110, 80-90 &  60-70 Kda probably due to proteolytic cleavages within the ECD of CD93

 

Calculated PI: 5.44

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 95820

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.