Human CD89/FCAR/FcalphaRI Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA
FCAR (Fc alpha receptor) or CD89 is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily (IgSF). ICD89 contains two Ig-like C2-type domains in the extracellular region. CD89 binds to the Fc region of immunoglobulins alpha (IgA) and mediates several functions including cytokine production. CD89 associates with ITAMcontaining signaling subunit FcRγ (Ig epsilon receptor subunit gamma) to form a functional signaling complex. CD89 is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. CD89 recognizes IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity (ADCC), and stimulation of the release of inflammatory mediators. There are multiple alternatively splicing variants encoding different isoforms of CD89.
Gene Symbol: CD89; FCAR; FcalphaRI; CTB-61M7.2
NCBI Gene ID: 2204
Uniprot Entry: P24071
Construct Details: Full length human CD89 geneis subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 864 (bp)
Gene Insert Sequence:
ATGGACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAG
GGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAAT
CCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAG
ATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACA
AGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGGTTCCGGTACAGTGACACCCTGGAGCT
GGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAG
AATATTTCCCTCACGTGCAGCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAAC
TTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTTGGGTCCTGTGGACCTCAATGT
CTCAGGGATCTACAGGTGCTACGGTTGGTACAACAGGAGCCCCTACCTGTGGTCCTTCCCCAGTAATGCC
TTGGAGCTTGTGGTCACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGG
CAGGACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAA
GGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGGATTGACCTTTGCA
CGAACACCAAGTGTCTGCAAGTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.