Human IL27RA/IL27R/CRL1/TCCR/WSX1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

IL27RA (interleukin-27 receptor subunit alpha) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL27RA consists of a extracellular region with 3 fibronectin type III (FN3) domains, and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  IL27RA is similar to the mouse T-cell cytokine receptor TCCR.  IL27RA acts as a receptor for IL27 but requires IL6ST/gp130 to mediate signal transduction in response to IL27. This IL27 signaling pathway that acts through STAT3 and STAT1 is involved in the regulation of Th1-type immune responses. It is also involved in innate defense mechanisms.

 

 

Gene Symbol: IL27RA; CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1

 

NCBI Gene ID: 9466

 

Uniprot Entry: Q6UWB1

 

Construct Details: Full length human IL27RA gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1911 (bp)

 

Gene Insert Sequence:  

ATGCGGGGAGGCAGGGGCGCCCCTTTCTGGCTGTGGCCGCTGCCCAAGCTGGCGCTGCTGCCTCTGTTGT

GGGTGCTTTTCCAGCGGACGCGTCCCCAGGGCAGCGCCGGGCCACTGCAGTGCTACGGAGTTGGACCCTT

GGGCGACTTGAACTGCTCGTGGGAGCCTCTTGGGGACCTGGGAGCCCCCTCCGAGTTACACCTCCAGAGC

CAAAAGTACCGTTCCAACAAAACCCAGACTGTGGCAGTGGCAGCCGGACGGAGCTGGGTGGCCATTCCTC

GGGAACAGCTCACCATGTCTGACAAACTCCTTGTCTGGGGCACTAAGGCAGGCCAGCCTCTCTGGCCCCC

CGTCTTCGTGAACCTAGAAACCCAAATGAAGCCAAACGCCCCCCGGCTGGGCCCTGACGTGGACTTTTCC

GAGGATGACCCCCTGGAGGCCACTGTCCATTGGGCCCCACCTACATGGCCATCTCATAAAGTTCTGATCT

GCCAGTTCCACTACCGAAGATGTCAGGAGGCGGCCTGGACCCTGCTGGAACCGGAGCTGAAGACCATACC

CCTGACCCCTGTTGAGATCCAAGATTTGGAGCTAGCCACTGGCTACAAAGTGTATGGCCGCTGCCGGATG

GAGAAAGAAGAGGATTTGTGGGGCGAGTGGAGCCCCATTTTGTCCTTCCAGACACCGCCTTCTGCTCCAA

AAGATGTGTGGGTATCAGGGAACCTCTGTGGGACGCCTGGAGGAGAGGAACCTTTGCTTCTATGGAAGGC

CCCAGGGCCCTGTGTGCAGGTGAGCTACAAAGTCTGGTTCTGGGTTGGAGGTCGTGAGCTGAGTCCAGAA

GGAATTACCTGCTGCTGCTCCCTAATTCCCAGTGGGGCGGAGTGGGCCAGGGTGTCCGCTGTCAACGCCA

CAAGCTGGGAGCCTCTCACCAACCTCTCTTTGGTCTGCTTGGATTCAGCCTCTGCCCCCCGTAGCGTGGC

AGTCAGCAGCATCGCTGGGAGCACGGAGCTACTGGTGACCTGGCAACCGGGGCCTGGGGAACCACTGGAG

CATGTAGTGGACTGGGCTCGAGATGGGGACCCCCTGGAGAAACTCAACTGGGTCCGGCTTCCCCCTGGGA

ACCTCAGTGCTCTGTTACCAGGGAATTTCACTGTCGGGGTCCCCTATCGAATCACTGTGACCGCAGTCTC

TGCTTCAGGCTTGGCCTCTGCATCCTCCGTCTGGGGGTTCAGGGAGGAATTAGCACCCCTAGTGGGGCCA

ACGCTTTGGCGACTCCAAGATGCCCCTCCAGGGACCCCCGCCATAGCGTGGGGAGAGGTCCCAAGGCACC

AGCTTCGAGGCCACCTCACCCACTACACCTTGTGTGCACAGAGTGGAACCAGCCCCTCCGTCTGCATGAA

TGTGAGTGGCAACACACAGAGTGTCACCCTGCCTGACCTTCCTTGGGGTCCCTGTGAGCTGTGGGTGACA

GCATCTACCATCGCTGGACAGGGCCCTCCTGGTCCCATCCTCCGGCTTCATCTACCAGATAACACCCTGA

GGTGGAAAGTTCTGCCGGGCATCCTATTCTTGTGGGGCTTGTTCCTGTTGGGGTGTGGCCTGAGCCTGGC

CACCTCTGGAAGGTGCTACCACCTAAGGCACAAAGTGCTGCCCCGCTGGGTCTGGGAGAAAGTTCCTGAT

CCTGCCAACAGCAGTTCAGGCCAGCCCCACATGGAGCAAGTACCTGAGGCCCAGCCCCTTGGGGACTTGC

CCATCCTGGAAGTGGAGGAGATGGAGCCCCCGCCGGTTATGGAGTCCTCCCAGCCCGCCCAGGCCACCGC

CCCGCTTGACTCTGGGTATGAGAAGCACTTCCTGCCCACACCTGAGGAGCTGGGCCTTCTGGGGCCCCCC

AGGCCACAGGTTCTGGCCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.