Human OSMR/IL31RB/OSMRB/PLCA1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

OSMR (oncostatin M receptor), also known as OSMRB (OSMR subunit beta) and IL31RB (IL-31 receptor subunit beta), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  OSMR consists of an extracellular region with 4 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  OSMR heterodimerizes with IL6ST (interleukin 6 signal transducer) or gp130 to form the type II OSMR complex and with IL31RA to form the IL31R complex, and thus transduces oncostatin M and IL31 induced signaling events.  Mutations in the OSMR gene have been associated with familial primary localized cutaneous amyloidosis (PLCA1). 

 

 

Gene Symbol: OSMR; OSMRB; IL31RB; PLCA1; IL-31RB; IL-31R-beta

 

NCBI Gene ID: 9180

 

Uniprot Entry: Q99650

 

Construct Details: Full length human OSMR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2940 (bp)

 

Gene Insert Sequence:  

ATGGCTCTATTTGCAGTCTTTCAGACAACATTCTTCTTAACATTGCTGTCCTTGAGGACTTACCAGAGTG

AAGTCTTGGCTGAACGTTTACCATTGACTCCTGTATCACTTAAAGTTTCCACCAATTCTACGCGTCAGAG

TTTGCACTTACAATGGACTGTCCACAACCTTCCTTATCATCAGGAATTGAAAATGGTATTTCAGATCCAG

ATCAGTAGGATTGAAACATCCAATGTCATCTGGGTGGGGAATTACAGCACCACTGTGAAGTGGAACCAGG

TTCTGCATTGGAGCTGGGAATCTGAGCTCCCTTTGGAATGTGCCACACACTTTGTAAGAATAAAGAGTTT

GGTGGACGATGCCAAGTTCCCTGAGCCAAATTTCTGGAGCAACTGGAGTTCCTGGGAGGAAGTCAGTGTA

CAAGATTCTACTGGACAGGATATATTGTTCGTTTTCCCTAAAGATAAGCTGGTGGAAGAAGGCACCAATG

TTACCATTTGTTACGTTTCTAGGAACATTCAAAATAATGTATCCTGTTATTTGGAAGGGAAACAGATTCA

TGGAGAACAACTTGATCCACATGTAACTGCATTCAACTTGAATAGTGTGCCTTTCATTAGGAATAAAGGG

ACAAATATCTATTGTGAGGCAAGTCAAGGAAATGTCAGTGAAGGCATGAAAGGCATCGTTCTTTTTGTCT

CAAAAGTACTTGAGGAGCCCAAGGACTTTTCTTGTGAAACCGAGGACTTCAAGACTTTGCACTGTACTTG

GGATCCTGGGACGGACACTGCCTTGGGGTGGTCTAAACAACCTTCCCAAAGCTACACTTTATTTGAATCA

TTTTCTGGGGAAAAGAAACTTTGTACACACAAAAACTGGTGTAATTGGCAAATAACTCAAGACTCACAAG

AAACCTATAACTTCACACTCATAGCTGAAAATTACTTAAGGAAGAGAAGTGTCAATATCCTTTTTAACCT

GACTCATCGAGTTTATTTAATGAATCCTTTTAGTGTCAACTTTGAAAATGTAAATGCCACAAATGCCATC

ATGACCTGGAAGGTGCACTCCATAAGGAATAATTTCACATATTTGTGTCAGATTGAACTCCATGGTGAAG

GAAAAATGATGCAATACAATGTTTCCATCAAGGTGAACGGTGAGTACTTCTTAAGTGAACTGGAACCTGC

CACAGAGTACATGGCGCGAGTACGGTGTGCTGATGCCAGCCACTTCTGGAAATGGAGTGAATGGAGTGGT

CAGAACTTCACCACACTTGAAGCTGCTCCCTCAGAGGCCCCTGATGTCTGGAGAATTGTGAGCTTGGAGC

CAGGAAATCATACTGTGACCTTATTCTGGAAGCCATTATCAAAACTGCATGCCAATGGAAAGATCCTGTT

CTATAATGTAGTTGTAGAAAACCTAGACAAACCATCCAGTTCAGAGCTCCATTCCATTCCAGCACCAGCC

AACAGCACAAAACTAATCCTTGACAGGTGTTCCTACCAAATCTGCGTCATAGCCAACAACAGTGTGGGTG

CTTCTCCTGCTTCTGTAATAGTCATCTCTGCAGACCCCGAAAACAAAGAGGTTGAGGAAGAAAGAATTGC

AGGCACAGAGGGTGGATTCTCTCTGTCTTGGAAACCCCAACCTGGAGATGTTATAGGCTATGTTGTGGAC

TGGTGTGACCATACCCAGGATGTGCTCGGTGATTTCCAGTGGAAGAATGTAGGTCCCAATACCACAAGCA

CAGTCATTAGCACAGATGCTTTTAGGCCAGGAGTTCGATATGACTTCAGAATTTATGGGTTATCTACAAA

AAGGATTGCTTGTTTATTAGAGAAAAAAACAGGATACTCTCAGGAACTTGCTCCTTCAGACAACCCTCAC

GTGCTGGTGGATACATTGACATCCCACTCCTTCACTCTGAGTTGGAAAGATTACTCTACTGAATCTCAAC

CTGGTTTTATACAAGGGTACCATGTCTATCTGAAATCCAAGGCGAGGCAGTGCCACCCACGATTTGAAAA

GGCAGTTCTTTCAGATGGTTCAGAATGTTGCAAATACAAAATTGACAACCCGGAAGAAAAGGCATTGATT

GTGGACAACCTAAAGCCAGAATCCTTCTATGAGTTTTTCATCACTCCATTCACTAGTGCTGGTGAAGGCC

CCAGTGCTACGTTCACGAAGGTCACGACTCCGGATGAACACTCCTCGATGCTGATTCATATCCTACTGCC

CATGGTTTTCTGCGTCTTGCTCATCATGGTCATGTGCTACTTGAAAAGTCAGTGGATCAAGGAGACCTGT

TATCCTGACATCCCTGACCCTTACAAGAGCAGCATCCTGTCATTAATAAAATTCAAGGAGAACCCTCACC

TAATAATAATGAATGTCAGTGACTGTATCCCAGATGCTATTGAAGTTGTAAGCAAGCCAGAAGGGACAAA

GATACAGTTCCTAGGCACTAGGAAGTCACTCACAGAAACCGAGTTGACTAAGCCTAACTACCTTTATCTC

CTTCCAACAGAAAAGAATCACTCTGGCCCTGGCCCCTGCATCTGTTTTGAGAACTTGACCTATAACCAGG

CAGCTTCTGACTCTGGCTCTTGTGGCCATGTTCCAGTATCCCCAAAAGCCCCAAGTATGCTGGGACTAAT

GACCTCACCTGAAAATGTACTAAAGGCACTAGAAAAAAACTACATGAACTCCCTGGGAGAAATCCCAGCT

GGAGAAACAAGTTTGAATTATGTGTCCCAGTTGGCTTCACCCATGTTTGGAGACAAGGACAGTCTCCCAA

CAAACCCAGTAGAGGCACCACACTGTTCAGAGTATAAAATGCAAATGGCAGTCTCCCTGCGTCTTGCCTT

GCCTCCCCCGACCGAGAATAGCAGCCTCTCCTCAATTACCCTTTTAGATCCAGGTGAACACTACTGCTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.