Human CNTFR/CNTFRA Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CNTFR or CNTFRa (Ciliary neurotrophic factor receptor subunit alpha) is a GPI-anchored surface glycoprotein of the type I cytokine receptor family, type 3 subfamily.  CNTFR consists of an extracellular region with 1 Ig-like C2-type (immunoglobulin-like) domain, 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, in addition to a transmembrane (TM) domain, and a cytoplasmic tail. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding. CNTF is one of the cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 that utilize the common signaling transducer IL6ST/gp130 for initiating signal transmission. Binding of CNTF to CNTFR recruits the transmembrane components of the co-receptors, IL6ST/gp130 and leukemia inhibitory factor receptor (LIFR), facilitating signal transduction. The CNTF signaling pathway plays a critical role in neuronal cell survival, differentiation and gene expression. Single nucleotide polymorphisms in the CNTFR gene may be associated with variations in muscle strength, as well as early onset of eating disorders. 

 

 

Gene Symbol: CNTFR; CNTFRA; CNTFR-alpha

 

NCBI Gene ID: 1271

 

Uniprot Entry: P26992

 

Construct Details: Full length human CNTFR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1119 (bp)

 

Gene Insert Sequence:  

ATGGCTGCTCCTGTCCCGTGGGCCTGCTGTGCTGTGCTTGCCGCCGCCGCCGCAGTTGTCTACGCCCAGA

GACACAGTCCACAGGAGGCACCCCATGTGCAGTACGAGCGCCTGGGCTCTGACGTGACACTGCCATGTGG

GACAGCAAACTGGGATGCTGCGGTGACGTGGCGGGTAAATGGGACAGACCTGGCCCCTGACCTGCTCAAC

GGCTCTCAGCTGGTGCTCCATGGCCTGGAACTGGGCCACAGTGGCCTCTACGCCTGCTTCCACCGTGACT

CCTGGCACCTGCGCCACCAAGTCCTGCTGCATGTGGGCTTGCCGCCGCGGGAGCCTGTGCTCAGCTGCCG

CTCCAACACTTACCCCAAGGGCTTCTACTGCAGCTGGCATCTGCCCACCCCCACCTACATTCCCAACACC

TTCAATGTGACTGTGCTGCATGGCTCCAAAATTATGGTCTGTGAGAAGGACCCAGCCCTCAAGAACCGCT

GCCACATTCGCTACATGCACCTGTTCTCCACCATCAAGTACAAGGTCTCCATAAGTGTCAGCAATGCCCT

GGGCCACAATGCCACAGCTATCACCTTTGACGAGTTCACCATTGTGAAGCCTGATCCTCCAGAAAATGTG

GTAGCCCGGCCAGTGCCCAGCAACCCTCGCCGGCTGGAGGTGACGTGGCAGACCCCCTCGACCTGGCCTG

ACCCTGAGTCTTTTCCTCTCAAGTTCTTTCTGCGCTACCGACCCCTCATCCTGGACCAGTGGCAGCATGT

GGAGCTGTCCGACGGCACAGCACACACCATCACAGATGCCTACGCCGGGAAGGAGTACATTATCCAGGTG

GCAGCCAAGGACAATGAGATTGGGACATGGAGTGACTGGAGCGTAGCCGCCCACGCTACGCCCTGGACTG

AGGAACCGCGACACCTCACCACGGAGGCCCAGGCTGCGGAGACCACGACCAGCACCACCAGCTCCCTGGC

ACCCCCACCTACCACGAAGATCTGTGACCCTGGGGAGCTGGGCAGCGGCGGGGGACCCTCGGCACCCTTC

TTGGTCAGCGTCCCCATCACTCTGGCCCTGGCTGCCGCTGCCGCCACTGCCAGCAGTCTCTTGATCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.