Human CD93/C1QR1/CDw93/MXRA4 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD93 is a single-pass type I transmembrane protein and a member of the calcium dependent C-type lectin superfamily.  CD93 contains 1 C-type lectin domain and 5 EGF-like domains in the extracellular region. CD93 was originally identified as a myeloid cell-specific marker and was thought to be a receptor for C1q.  CD93 may be involved in intercellular adhesion and in the clearance of apoptotic cells. CD93 is highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils but not expressed in cells of lymphoid origin. CD93 interacts with HCV core protein.  CD93 also binds C1QBP; the association may represent a cell surface C1q receptor. The intracellular cytoplasmic tail of CD93 may interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.  

 

 

Gene Symbol: CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1

 

NCBI Gene ID: 22918

 

Uniprot Entry: O9NPY3

 

Construct Details: Full length human CD93 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1959 (bp)

 

Gene Insert Sequence:  

ATGGCCACCTCCATGGGCCTGCTGCTGCTGCTGCTGCTGCTCCTGACCCAGCCCGGGGCGGGGACGGGAG

CTGACACGGAGGCGGTGGTCTGCGTGGGGACCGCCTGCTACACGGCCCACTCGGGCAAGCTGAGCGCTGC

CGAGGCCCAGAACCACTGCAACCAGAACGGGGGCAACCTGGCCACTGTGAAGAGCAAGGAGGAGGCCCAG

CACGTCCAGCGAGTACTGGCCCAGCTCCTGAGGCGGGAGGCAGCCCTGACGGCGAGGATGAGCAAGTTCT

GGATTGGGCTCCAGCGAGAGAAGGGCAAGTGCCTGGACCCTAGTCTGCCGCTGAAGGGCTTCAGCTGGGT

GGGCGGGGGGGAGGACACGCCTTACTCTAACTGGCACAAGGAGCTCCGGAACTCGTGCATCTCCAAGCGC

TGTGTGTCTCTGCTGCTGGACCTGTCCCAGCCGCTCCTTCCCAGCCGCCTCCCCAAGTGGTCTGAGGGCC

CCTGTGGGAGCCCAGGCTCCCCCGGAAGTAACATTGAGGGCTTCGTGTGCAAGTTCAGCTTCAAAGGCAT

GTGCCGGCCTCTGGCCCTGGGGGGCCCAGGTCAGGTGACCTACACCACCCCCTTCCAGACCACCAGTTCC

TCCTTGGAGGCTGTGCCCTTTGCCTCTGCGGCCAATGTAGCCTGTGGGGAAGGTGACAAGGACGAGACTC

AGAGTCATTATTTCCTGTGCAAGGAGAAGGCCCCCGATGTGTTCGACTGGGGCAGCTCGGGCCCCCTCTG

TGTCAGCCCCAAGTATGGCTGCAACTTCAACAATGGGGGCTGCCACCAGGACTGCTTTGAAGGGGGGGAT

GGCTCCTTCCTCTGCGGCTGCCGACCAGGATTCCGGCTGCTGGATGACCTGGTGACCTGTGCCTCTCGAA

ACCCTTGCAGCTCCAGCCCATGTCGTGGGGGGGCCACGTGCGTCCTGGGACCCCATGGGAAAAACTACAC

GTGCCGCTGCCCCCAAGGGTACCAGCTGGACTCGAGTCAGCTGGACTGTGTGGACGTGGATGAATGCCAG

GACTCCCCCTGTGCCCAGGAGTGTGTCAACACCCCTGGGGGCTTCCGCTGCGAATGCTGGGTTGGCTATG

AGCCGGGCGGTCCTGGAGAGGGGGCCTGTCAGGATGTGGATGAGTGTGCTCTGGGTCGCTCGCCTTGCGC

CCAGGGCTGCACCAACACAGATGGCTCATTTCACTGCTCCTGTGAGGAGGGCTACGTCCTGGCCGGGGAG

GACGGGACTCAGTGCCAGGACGTGGATGAGTGTGTGGGCCCGGGGGGCCCCCTCTGCGACAGCTTGTGCT

TCAACACACAAGGGTCCTTCCACTGTGGCTGCCTGCCAGGCTGGGTGCTGGCCCCAAATGGGGTCTCTTG

CACCATGGGGCCTGTGTCTCTGGGACCACCATCTGGGCCCCCCGATGAGGAGGACAAAGGAGAGAAAGAA

GGGAGCACCGTGCCCCGTGCTGCAACAGCCAGTCCCACAAGGGGCCCCGAGGGCACCCCCAAGGCTACAC

CCACCACAAGTAGACCTTCGCTGTCATCTGACGCCCCCATCACATCTGCCCCACTCAAGATGCTGGCCCC

CAGTGGGTCCCCAGGCGTCTGGAGGGAGCCCAGCATCCATCACGCCACAGCTGCCTCTGGCCCCCAGGAG

CCTGCAGGTGGGGACTCCTCCGTGGCCACACAAAACAACGATGGCACTGACGGGCAAAAGCTGCTTTTAT

TCTACATCCTAGGCACCGTGGTGGCCATCCTACTCCTGCTGGCCCTGGCTCTGGGGCTACTGGTCTATCG

CAAGCGGAGAGCGAAGAGGGAGGAGAAGAAGGAGAAGAAGCCCCAGAATGCGGCAGACAGTTACTCCTGG

GTTCCAGAGCGAGCTGAGAGCAGGGCCATGGAGAACCAGTACAGTCCGACACCTGGGACAGACTGCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.