Human CD248/TEM1/CD164L1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD248, also known as TEM1 (tumor endothelial marker 1) and  CD164L1 (CD164 sialomucin-like 1) is a single-pass type I transmembrane protein and a member of the C-type lectin superfamily.  CD248 contains 1 C-type lectin domain, 1 EGF-like domain and 1 Sushi (CCP/SCR) domain in the extracellular region.  CD248 is expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. It is expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. CD248 is expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. However it is expressed in stromal fibroblasts  CD248 may play a role in tumor angiogenesis.

 

 

Gene Symbol: CD248; TEM1; CD164L1

 

NCBI Gene ID: 57124

 

Uniprot Entry: O9HCU0

 

Construct Details: Full length human CD248 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2274 (bp)

 

Gene Insert Sequence:  

ATGCTGCTGCGCCTGTTGCTGGCCTGGGCGGCCGCAGGGCCCACACTGGGCCAGGACCCCTGGGCTGCTG

AGCCCCGTGCCGCCTGCGGCCCCAGCAGCTGCTACGCTCTCTTCCCACGGCGCCGCACCTTCCTGGAGGC

CTGGCGGGCCTGCCGCGAGCTGGGGGGCGACCTGGCCACTCCTCGGACCCCCGAGGAGGCCCAGCGTGTG

GACAGCCTGGTGGGTGCGGGCCCAGCCAGCCGGCTGCTGTGGATCGGGCTGCAGCGGCAGGCCCGGCAAT

GCCAGCTGCAGCGCCCACTGCGCGGCTTCACGTGGACCACAGGGGACCAGGACACGGCTTTCACCAACTG

GGCCCAGCCAGCCTCTGGAGGCCCCTGCCCGGCCCAGCGCTGTGTGGCCCTGGAGGCAAGTGGCGAGCAC

CGCTGGCTGGAGGGCTCGTGCACGCTGGCTGTCGACGGCTACCTGTGCCAGTTTGGCTTCGAGGGCGCCT

GCCCGGCGCTGCAAGATGAGGCGGGCCAGGCCGGCCCAGCCGTGTATACCACGCCCTTCCACCTGGTCTC

CACAGAGTTTGAGTGGCTGCCCTTCGGCTCTGTGGCCGCTGTGCAGTGCCAGGCTGGCAGGGGAGCCTCT

CTGCTCTGCGTGAAGCAGCCTGAGGGAGGTGTGGGCTGGTCACGGGCTGGGCCCCTGTGCCTGGGGACTG

GCTGCAGCCCTGACAACGGGGGCTGCGAACACGAATGTGTGGAGGAGGTGGATGGTCACGTGTCCTGCCG

CTGCACTGAGGGCTTCCGGCTGGCAGCAGACGGGCGCAGTTGCGAGGACCCCTGTGCCCAGGCTCCGTGC

GAGCAGCAGTGTGAGCCCGGTGGGCCACAAGGCTACAGCTGCCACTGTCGCCTGGGTTTCCGGCCAGCGG

AGGATGATCCGCACCGCTGTGTGGACACAGATGAGTGCCAGATTGCCGGTGTGTGCCAGCAGATGTGTGT

CAACTACGTTGGTGGCTTCGAGTGTTATTGTAGCGAGGGACATGAGCTGGAGGCTGATGGCATCAGCTGC

AGCCCTGCAGGGGCCATGGGTGCCCAGGCTTCCCAGGACCTCGGAGATGAGTTGCTGGATGACGGGGAGG

ATGAGGAAGATGAAGACGAGGCCTGGAAGGCCTTCAACGGTGGCTGGACGGAGATGCCTGGGATCCTGTG

GATGGAGCCTACGCAGCCGCCTGACTTTGCCCTGGCCTATAGACCGAGCTTCCCAGAGGACAGAGAGCCA

CAGATACCCTACCCGGAGCCCACCTGGCCACCCCCGCTCAGTGCCCCCAGGGTCCCCTACCACTCCTCAG

TGCTCTCCGTCACCCGGCCTGTGGTGGTCTCTGCCACGCATCCCACACTGCCTTCTGCCCACCAGCCTCC

TGTGATCCCTGCCACACACCCAGCTTTGTCCCGTGACCACCAGATCCCCGTGATCGCAGCCAACTATCCA

GATCTGCCTTCTGCCTACCAACCCGGTATTCTCTCTGTCTCTCATTCAGCACAGCCTCCTGCCCACCAGC

CCCCTATGATCTCAACCAAATATCCGGAGCTCTTCCCTGCCCACCAGTCCCCCATGTTTCCAGACACCCG

GGTCGCTGGCACCCAGACCACCACTCATTTGCCTGGAATCCCACCTAACCATGCCCCTCTGGTCACCACC

CTCGGTGCCCAGCTACCCCCTCAAGCCCCAGATGCCCTTGTCCTCAGAACCCAGGCCACCCAGCTTCCCA

TTATCCCAACTGCCCAGCCCTCTCTGACCACCACCTCCAGGTCCCCTGTGTCTCCTGCCCATCAAATCTC

TGTGCCTGCTGCCACCCAGCCCGCAGCCCTCCCCACCCTCCTGCCCTCTCAGAGCCCCACTAACCAGACC

TCACCCATCAGCCCTACACATCCCCATTCCAAAGCCCCCCAAATCCCAAGGGAAGATGGCCCCAGTCCCA

AGTTGGCCCTGTGGCTGCCCTCACCAGCTCCCACAGCAGCCCCAACAGCCCTGGGGGAGGCTGGTCTTGC

CGAGCACAGCCAGAGGGATGACCGGTGGCTGCTGGTGGCACTCCTGGTGCCAACGTGTGTCTTTTTGGTG

GTCCTGCTTGCACTGGGCATCGTGTACTGCACCCGCTGTGGCCCCCATGCACCCAACAAGCGCATCACTG

ACTGCTATCGCTGGGTCATCCATGCTGGGAGCAAGAGCCCAACAGAACCCATGCCCCCCAGGGGCAGCCT

CACAGGGGTGCAGACCTGCAGAACCAGCGTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.