Human LILRA5/CD85f/LIR9/ILT11/LILRB7 Gene

Description

LILRA5 (Leukocyte immunoglobulin like receptor A5), also known as CD85F and ILT11 (Immunoglobulin-like transcript 11), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  LIR family members are known to have activating and inhibitory functions in leukocytes.  It belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail. LILRA5 contains 2 Ig-like C2 type domains in the extracellular region. LILRA5 may play a role in triggering innate immune responses. But it does not seem to play a role for any class I MHC antigen recognition. LILRA5 is expressed mostly in tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Among leukocytes, monocytes and neutrophils express the highest level. It is expressed in CD14+ monocytes, but not in T-cells, B-cells or natural killer (NK) cells Crosslink of LILRA5 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.

 

 

Gene Symbol: LILRA5; ILT11; CD85F; LIR9; ILT-11; LIR-9; LILRB7; CD85

 

NCBI Gene ID: 353514

 

Uniprot Entry: A6NI73

 

Construct Details: Full length human LILRA5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 900 (bp)

 

Gene Insert Sequence:  

ATGGCACCATGGTCTCATCCATCTGCACAGCTGCAGCCAGTGGGAGGAGACGCCGTGAGCCCTGCCCTCA

TGGTTCTGCTCTGCCTCGGGCTGAGTCTGGGCCCCAGGACCCACGTGCAGGCAGGGAACCTCTCCAAAGC

CACCCTCTGGGCTGAGCCAGGCTCTGTGATCAGCCGGGGGAACTCTGTGACCATCCGGTGTCAGGGGACC

CTGGAGGCCCAGGAATACCGTCTGGTTAAAGAGGGAAGCCCAGAACCCTGGGACACACAGAACCCACTGG

AGCCCAAGAACAAGGCCAGATTCTCCATCCCATCCATGACAGAGCACCATGCAGGGAGATACCGCTGTTA

CTACTACAGCCCTGCAGGCTGGTCAGAGCCCAGCGACCCCCTGGAGCTGGTGGTGACAGGATTCTACAAC

AAACCCACCCTCTCAGCCCTGCCCAGTCCTGTGGTGACCTCAGGAGAGAACGTGACCCTCCAGTGTGGCT

CACGGCTGAGATTCGACAGGTTCATTCTGACTGAGGAAGGAGACCACAAGCTCTCCTGGACCTTGGACTC

ACAGCTGACCCCCAGTGGGCAGTTCCAGGCCCTGTTCCCTGTGGGCCCTGTGACCCCCAGCCACAGGTGG

ATGCTCAGATGCTATGGCTCTCGCAGGCATATCCTGCAGGTATGGTCAGAACCCAGTGACCTCCTGGAGA

TTCCGGTCTCAGGAGCAGCTGATAACCTCAGTCCGTCACAAAACAAGTCTGACTCTGGGACTGCCTCACA

CCTTCAGGATTACGCAGTAGAGAATCTCATCCGCATGGGCATGGCCGGCTTGATCCTGGTGGTCCTTGGG

ATTCTGATATTTCAGGATTGGCACAGCCAGAGAAGCCCCCAAGCTGCAGCTGGAAGGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.