Human SIGIRR/TIR8 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Product ID: pLTC-SIGIRR (SKU#: LTP1308)

Price:

$896.00

Description

SIGIRR (single immunoglobulin IL-1 receptor-related molecule) is a single-pass type III membrane protein that belongs to the interleukin 1 receptor (IL1R) family. The IL1R family comprises at least 10 members, which typically contain 3 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. Although SIGIRR contains the conserved Ig-like and TOR motifs, it differs from the other 9 members by having only one Ig-like domain. Human SIGIRR lacks signal peptide and contains a cytoplasmic extention beyond the TIR domain, which is reminiscent of the structure of Toll and Tolllike receptor family members but absent in most IL1 receptor family members. SIGIRR is widely expressed in mouse and human epithelial tissues, such as kidney, lung and gut. The ligand and signaling mechanism for SIGIRR remain unclear. SIGIRR may act as a negative regulator for Toll-IL-1R signaling by trapping the crucial components of the pathway. Inflammation is enhanced in SIGIRR-deficient mice, as demonstrated by the increased chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge. SIGIRR may plays critical role in gut homeostasis, intestinal inflammation, and colitis-associated tumorigenesis by maintaining the microbial tolerance of the colonic epithelium. Moreover, SIGIRR-deficient mice developed stronger Th2 immune response, suggesting that SIGIRR is an important regulator of Th2, possibly through its impact on IL-33-mediated signaling pathway.

Gene Symbol: SIGIRR; TIR8; UNQ301/PRO342

NCBI Gene ID: 59307

Uniprot Entry: Q6IA17

Construct Details: Full length human SIGIRR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC or pLTC-Marker (see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1233 (bp)

Gene Insert Sequence:

ATGCCAGGTGTCTGTGATAGGGCCCCTGACTTCCTCTCCCCGTCTGAAGACCAGGTGCTGAGGCCTGCCTTGGGCAGCTC

AGTGGCTCTGAACTGCACGGCTTGGGTAGTCTCTGGGCCCCACTGCTCCCTGCCTTCAGTCCAGTGGCTGAAAGACGGGC

TTCCATTGGGAATTGGGGGCCACTACAGCCTCCACGAGTACTCCTGGGTCAAGGCCAACCTGTCAGAGGTGCTTGTGTCC

AGTGTCCTGGGGGTCAACGTGACCAGCACTGAAGTCTATGGGGCCTTCACCTGCTCCATCCAGAACATCAGCTTCTCCTC

CTTCACTCTTCAGAGAGCTGGCCCTACAAGCCACGTGGCTGCGGTGCTGGCCTCCCTCCTGGTCCTGCTGGCCCTGCTGC

TGGCCGCCCTGCTCTATGTCAAGTGCCGTCTCAACGTGCTGCTCTGGTACCAGGACGCGTATGGGGAGGTGGAGATAAAC

GACGGGAAGCTCTACGACGCCTACGTCTCCTACAGCGACTGCCCCGAGGACCGCAAGTTCGTGAACTTCATCCTAAAGCC

GCAGCTGGAGCGGCGTCGGGGCTACAAGCTCTTCCTGGACGACCGCGACCTCCTGCCGCGCGCTGAGCCCTCCGCCGACC

TCTTGGTGAACCTGAGCCGCTGCCGACGCCTCATCGTGGTGCTTTCGGACGCCTTCCTGAGCCGGGCCTGGTGCAGCCAC

AGCTTCCGGGAGGGCCTGTGCCGGCTGCTGGAGCTCACCCGCAGACCCATCTTCATCACCTTCGAGGGCCAGAGGCGCGA

CCCCGCGCACCCGGCGCTCCGCCTGCTGCGCCAGCACCGCCACCTGGTGACCTTGCTGCTCTGGAGGCCCGGCTCCGTGA

CTCCTTCCTCCGATTTTTGGAAAGAAGTGCAGCTGGCGCTGCCGCGGAAGGTGCGGTACAGGCCGGTGGAAGGAGACCCC

CAGACGCAGCTGCAGGACGACAAGGACCCCATGCTGATTCTTCGAGGCCGAGTCCCTGAGGGCCGGGCCCTGGACTCAGA

GGTGGACCCGGACCCTGAGGGCGACCTGGGTGTCCGGGGGCCTGTTTTTGGAGAGCCATCAGCTCCACCGCACACCAGTG

GGGTCTCGCTGGGAGAGAGCCGGAGCAGCGAAGTGGACGTCTCGGATCTCGGCTCGCGAAACTACAGTGCCCGCACAGAC

TTCTACTGCCTGGTGTCCAAGGATGATATGTAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

1. Cytokine 11: 389-399 (1999).

2. Nat. Immunol. 4:920-927 (2003).

3. J. Biol. Chem. 280:25233-25241 (2005).

4. Immunity . 26: 461-475 (2007).

5. J Immunol. 182: 2601-2609 (2009).

Product datasheet (pdf) can be downloaded here: LTP1308-PDS.pdf