Human IL1RAP/IL1R3 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Product ID: pLTC-IL1RAP (SKU#: LTP1288)

Price:

$1,096.00

Description

IL1RAP (interleukin-1 receptor accessory protein), also known as IL1R3 (interleukin-1 receptor 3), is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin-1 receptor (IL1R) family. Like other family members, IL1RAP contain 3 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL1RAP is identified as a co-receptor for a high-affinity IL-1 receptor type I (IL1R1) complex that mediates IL1-dependent activation of NF-κB, MAPK and other signaling pathways. IL1RAP is required for transducing the signaling events on the plasma membrane level to downstream activation of IL1-responsive genes. IL1 are pleiotropic cytokines that induce synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress. Alternative splicing of the IL1RAP gene results in 2 transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. The soluble form of IL1RAP contributes to the antagonism of IL-1 action. When present with soluble IL1R2, soluble IL1RAP increases the IL1 binding affinity y of IL1R2 more than 100fold. In addition, ILRAP interacts with ST2 on mast cells and Th2 T lymphocytes to create a functional receptor complex for transducing IL33-dependent signaling activity.

Gene Symbol: IL1RAP; IL-1RAP; IL1R3; IL-1R3; IL-1R-3; C3orf13; IL-1RAcP

NCBI Gene ID: 3556

Uniprot Entry: Q9NPH3

Construct Details: Full length human IL1RAP gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC or pLTC-Marker (see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1713 (bp)

Gene Insert Sequence:

ATGACACTTCTGTGGTGTGTAGTGAGTCTCTACTTTTATGGAATCCTGCAAAGTGATGCCTCAGAACGCTGCGATGACTG

GGGACTAGACACCATGAGGCAAATCCAAGTGTTTGAAGATGAGCCAGCTCGCATCAAGTGCCCACTCTTTGAACACTTCT

TGAAATTCAACTACAGCACAGCCCATTCAGCTGGCCTTACTCTGATCTGGTATTGGACTAGGCAGGACCGGGACCTTGAG

GAGCCAATTAACTTCCGCCTCCCCGAGAACCGCATTAGTAAGGAGAAAGATGTGCTGTGGTTCCGGCCCACTCTCCTCAA

TGACACTGGCAACTATACCTGCATGTTAAGGAACACTACATATTGCAGCAAAGTTGCATTTCCCTTGGAAGTTGTTCAAA

AAGACAGCTGTTTCAATTCCCCCATGAAACTCCCAGTGCATAAACTGTATATAGAATATGGCATTCAGAGGATCACTTGT

CCAAATGTAGATGGATATTTTCCTTCCAGTGTCAAACCGACTATCACTTGGTATATGGGCTGTTATAAAATACAGAATTT

TAATAATGTAATACCCGAAGGTATGAACTTGAGTTTCCTCATTGCCTTAATTTCAAATAATGGAAATTACACATGTGTTG

TTACATATCCAGAAAATGGACGTACGTTTCATCTCACCAGGACTCTGACTGTAAAGGTAGTAGGCTCTCCAAAAAATGCA

GTGCCCCCTGTGATCCATTCACCTAATGATCATGTGGTCTATGAGAAAGAACCAGGAGAGGAGCTACTCATTCCCTGTAC

GGTCTATTTTAGTTTTCTGATGGATTCTCGCAATGAGGTTTGGTGGACCATTGATGGAAAAAAACCTGATGACATCACTA

TTGATGTCACCATTAACGAAAGTATAAGTCATAGTAGAACAGAAGATGAAACAAGAACTCAGATTTTGAGCATCAAGAAA

GTTACCTCTGAGGATCTCAAGCGCAGCTATGTCTGTCATGCTAGAAGTGCCAAAGGCGAAGTTGCCAAAGCAGCCAAGGT

GAAGCAGAAAGTGCCAGCTCCAAGATACACAGTGGAACTGGCTTGTGGTTTTGGAGCCACAGTCCTGCTAGTGGTGATTC

TCATTGTTGTTTACCATGTTTACTGGCTAGAGATGGTCCTATTTTACCGGGCTCATTTTGGAACAGATGAAACCATTTTA

GATGGAAAAGAGTATGATATTTATGTATCCTATGCAAGGAATGCGGAAGAAGAAGAATTTGTATTACTGACCCTCCGTGG

AGTTTTGGAGAATGAATTTGGATACAAGCTGTGCATCTTTGACCGAGACAGTCTGCCTGGGGGAATTGTCACAGATGAGA

CTTTGAGCTTCATTCAGAAAAGCAGACGCCTCCTGGTTGTTCTAAGCCCCAACTACGTGCTCCAGGGAACCCAAGCCCTC

CTGGAGCTCAAGGCTGGCCTAGAAAATATGGCCTCTCGGGGCAACATCAACGTCATTTTAGTACAGTACAAAGCTGTGAA

GGAAACGAAGGTGAAAGAGCTGAAGAGGGCTAAGACGGTGCTCACGGTCATTAAATGGAAAGGGGAAAAATCCAAGTATC

CACAGGGCAGGTTCTGGAAGCAGCTGCAGGTGGCCATGCCAGTGAAGAAAAGTCCCAGGCGGTCTAGCAGTGATGAGCAG

GGCCTCTCGTATTCATCTTTGAAAAATGTATGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

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3. J. Immunol. 161:6871 (1998).

4. J. Immunol. 160:3170 (1998).

5. Immunity, 18: 87-96 (2003).

Product datasheet (pdf) can be downloaded here: LTP1288-PDS.pdf