Human CD131/CSF2RB/IL3RB/IL5RB/SMDP5 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD131, also known as CSF2RB (colony-stimulating factor-2 receptor beta), IL3RB and IL5RB (IL-3/IL-5 receptor common beta), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 4 subfamily.  CD131 consists of a extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD131 is a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF to mediate their cellular responses.  For example, the CSF receptor complex is composed of a cytokine-specific alpha- chain CD116 and a beta-chain CD131 that are shared with the receptors for IL-3 and IL-5. CD116 alone binds GM-CSF with low affinity, whereas CD131 does not bind GM-CSF by itself. Association with CD131 converts CD116 from a low affinity to a high affinity receptor for GM­CSF. GM­CSF is a hematopoietic cytokine that regulates differentiation, survival, and proliferation of colony-forming unit-granulocyte-macrophage progenitor cells. Mutations in CD131 may cause pulmonary surfactant metabolism dysfunction 5 (SMDP5).

 

 

Gene Symbol: CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131

 

NCBI Gene ID: 1439

 

Uniprot Entry: P32927

 

Construct Details: Full length human CD131/CSF2RB gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2694 (bp)

 

Gene Insert Sequence:  

ATGGTGCTGGCCCAGGGGCTGCTCTCCATGGCCCTGCTGGCCCTGTGCTGGGAGCGCAGCCTGGCAGGGG

CAGAAGAAACCATCCCGCTGCAGACCCTGCGCTGCTACAACGACTACACCAGCCACATCACCTGCAGGTG

GGCAGACACCCAGGATGCCCAGCGGCTCGTCAACGTGACCCTCATTCGCCGGGTGAATGAGGACCTCCTG

GAGCCAGTGTCCTGTGACCTCAGTGATGACATGCCCTGGTCAGCCTGCCCCCATCCCCGCTGCGTGCCCA

GGAGATGTGTCATTCCCTGCCAGAGTTTTGTCGTCACTGACGTTGACTACTTCTCATTCCAACCAGACAG

GCCTCTGGGCACCCGGCTCACCGTCACTCTGACCCAGCATGTCCAGCCTCCTGAGCCCAGGGACCTGCAG

ATCAGCACCGACCAGGACCACTTCCTGCTGACCTGGAGTGTGGCCCTTGGGAGTCCCCAGAGCCACTGGT

TGTCCCCAGGGGATCTGGAGTTTGAGGTGGTCTACAAGCGGCTTCAGGACTCTTGGGAGGACGCAGCCAT

CCTCCTCTCCAACACCTCCCAGGCCACCCTGGGGCCAGAGCACCTCATGCCCAGCAGCACCTACGTGGCC

CGAGTACGGACCCGCCTGGCCCCAGGTTCTCGGCTCTCAGGACGTCCCAGCAAGTGGAGCCCAGAGGTTT

GCTGGGACTCCCAGCCAGGGGATGAGGCCCAGCCCCAGAACCTGGAGTGCTTCTTTGACGGGGCCGCCGT

GCTCAGCTGCTCCTGGGAGGTGAGGAAGGAGGTGGCCAGCTCGGTCTCCTTTGGCCTATTCTACAAGCCC

AGCCCAGATGCAGGGGAGGAAGAGTGCTCCCCAGTGCTGAGGGAGGGGCTCGGCAGCCTCCACACCAGGC

ACCACTGCCAGATTCCCGTGCCCGACCCCGCGACCCACGGCCAATACATCGTCTCTGTTCAGCCAAGGAG

GGCAGAGAAACACATAAAGAGCTCAGTGAACATCCAGATGGCCCCTCCATCCCTCAACGTGACCAAGGAT

GGAGACAGCTACAGCCTGCGCTGGGAAACAATGAAAATGCGATACGAACACATAGACCACACATTTGAGA

TCCAGTACAGGAAAGACACGGCCACGTGGAAGGACAGCAAGACCGAGACCCTCCAGAACGCCCACAGCAT

GGCCCTGCCAGCCCTGGAGCCCTCCACCAGGTACTGGGCCAGGGTGAGGGTCAGGACCTCCCGCACCGGC

TACAACGGGATCTGGAGCGAGTGGAGTGAGGCGCGCTCCTGGGACACCGAGTCGGTGCTGCCTATGTGGG

TGCTGGCCCTCATCGTGATCTTCCTCACCATCGCTGTGCTCCTGGCCCTCCGCTTCTGTGGCATCTACGG

GTACAGGCTGCGCAGAAAGTGGGAGGAGAAGATCCCCAACCCCAGCAAGAGCCACCTGTTCCAGAACGGG

AGCGCAGAGCTTTGGCCCCCAGGCAGCATGTCGGCCTTCACTAGCGGGAGTCCCCCACACCAGGGGCCGT

GGGGCAGCCGCTTCCCTGAGCTGGAGGGGGTGTTCCCTGTAGGATTCGGGGACAGCGAGGTGTCACCTCT

CACCATAGAGGACCCCAAGCATGTCTGTGATCCACCATCTGGGCCTGACACGACTCCAGCTGCCTCAGAT

CTACCCACAGAGCAGCCCCCCAGCCCCCAGCCAGGCCCGCCTGCCGCCTCCCACACACCTGAGAAACAGG

CTTCCAGCTTTGACTTCAATGGGCCCTACCTGGGGCCGCCCCACAGCCGCTCCCTACCTGACATCCTGGG

CCAGCCGGAGCCCCCACAGGAGGGTGGGAGCCAGAAGTCCCCACCTCCAGGGTCCCTGGAGTACCTGTGT

CTGCCTGCTGGGGGGCAGGTGCAACTGGTCCCTCTGGCCCAGGCGATGGGACCAGGACAGGCCGTGGAAG

TGGAGAGAAGGCCGAGCCAGGGGGCTGCAGGGAGTCCCTCCCTGGAGTCCGGGGGAGGCCCTGCCCCTCC

TGCTCTTGGGCCAAGGGTGGGAGGACAGGACCAAAAGGACAGCCCTGTGGCTATACCCATGAGCTCTGGG

GACACTGAGGACCCTGGAGTGGCCTCTGGTTATGTCTCCTCTGCAGACCTGGTATTCACCCCAAACTCAG

GGGCCTCGTCTGTCTCCCTAGTTCCCTCTCTGGGCCTCCCCTCAGACCAGACCCCCAGCTTATGTCCTGG

GCTGGCCAGTGGACCCCCTGGAGCCCCAGGCCCTGTGAAGTCAGGGTTTGAGGGCTATGTGGAGCTCCCT

CCAATTGAGGGCCGGTCCCCCAGGTCACCAAGGAACAATCCTGTCCCCCCTGAGGCCAAAAGCCCTGTCC

TGAACCCAGGGGAACGCCCGGCAGATGTGTCCCCAACATCCCCACAGCCCGAGGGCCTCCTTGTCCTGCA

GCAAGTGGGCGACTATTGCTTCCTCCCCGGCCTGGGGCCCGGCCCTCTCTCGCTCCGGAGTAAACCTTCT

TCCCCGGGACCCGGTCCTGAGATCAAGAACCTAGACCAGGCTTTTCAAGTCAAGAAGCCCCCAGGCCAGG

CTGTGCCCCAGGTGCCCGTCATTCAGCTCTTCAAAGCCCTGAAGCAGCAGGACTACCTGTCTCTGCCCCC

TTGGGAGGTCAACAAGCCTGGGGAGGTGTGTTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Proc. Natl. Acad. Sci. 87:9655 (1990)

2. J. Clin. Invest. 100:2211-2217(1997)

3. J. Mol. Biol. 297:989-1001(2000)

4. Cell 104:291-300(2001)

5. J. Med. Genet. 48:205-209(2011) 

 

 

 

Additional supporting documents, including Product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.