Human CD6/TP120/T12 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
CD6, also known as TP120, is a single-pass type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 contains three SRCR domains in the extracellular region. CD6 is a cell surface glycoprotein expressed primarily on T cells and thus a T-cell differentiation antigen. CD6 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some neuronal cells in the brain. CD6 may function as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. As a cell adhesion molecule, CD6 mediates cell-cell contacts and regulates T cell responses via its interaction with the ligand ALCAM (activated leukocyte cell adhesion molecule) or CD166. CD6 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse on T lymphocytes. CD6/ALCAM interactions are thought to play a role in thymocyte development. CD6 may also contribute to signaling cascades triggered by activation of the TCR/CD3 complex and the overall functional avidity of selection. CD6 also functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. CD6 binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. CD6 thus mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 may be associated with susceptibility to multiple sclerosis
Gene Symbol: CD6; TP120; T12
NCBI Gene ID: 923
Uniprot Entry: P30203
Construct Details: The recombinant human CD6-Fc is expressed as a 619-amino acid protein consisting of His18 - Glu398 region of CD6 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing human CD6-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
HPSPAPPDQL NTSSAESELW EPGERLPVRL TNGSSSCSGT VEVRLEASWE PACGALWDSR
70 80 90 100 110 120
AAEAVCRALG CGGAEAASQL APPTPELPPP PAAGNTSVAA NATLAGAPAL LCSGAEWRLC
130 140 150 160 170 180
EVVEHACRSD GRRARVTCAE NRALRLVDGG GACAGRVEML EHGEWGSVCD DTWDLEDAHV
190 200 210 220 230 240
VCRQLGCGWA VQALPGLHFT PGRGPIHRDQ VNCSGAEAYL WDCPGLPGQH YCGHKEDAGA
250 260 270 280 290 300
VCSEHQSWRL TGGADRCEGQ VEVHFRGVWN TVCDSEWYPS EAKVLCQSLG CGTAVERPKG
310 320 330 340 350 360
LPHSLSGRMY YSCNGEELTL SNCSWRFNNS NLCSQSLAAR VLCSASRSLH NLSTPEVPAS
370 380 390 400 410 420
VQTVTIESSV TVKIENKESR ESTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK
430 440 450 460 470 480
DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV
490 500 510 520 530 540
LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL
550 560 570 580 590 600
VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM
610
HEALHNHYTQ KSLSLSPGK
M.W.: Calculated molecular mass (kDa): 67.3; Estimated by SDS-PAGE under reducing condition (kDa): 90-100.
Calculated PI: 5.62
Calculated extinction coefficients (M-1 cm-1, at 280nm): 112225
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")
Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity: Binds to its ligand ALCAM/CD166 and neutralizes ALCAM/CD166-mediated signaling activity.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
1. J. Immunol. 143:2439 (1989)
2. J. Exp. Med. 174:949 (1991)
3. Eur. J. Immunol. 25:2765 (1995)
4. J. Immunol. 158:1149 (1997)
5. J. Immunol. 178:4351 (2007)
6. Proc. Natl. Acad. Sci. 104:11724 (2007)
Product datasheet (pdf) can be downloaded here: FCL2579-PDS.pdf
Additional supporting documents, including COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.