Human PTCH/PTCH1/PTC/PTC1/Patched Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

PTCH/PTCH1/PTC/PTC1 is a member of the patched (PTCH) gene family. The PTCH gene encodes a 12-pass tranmembrrane protein containing 1 SSD (sterol-sensing) domain.  PTCH acts as a receptor for sonic hedgehog (SHH), a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as indian hedgehog (IHH) and desert hedgehog (DHH). PTCH also associates with the smoothened (SMO) protien to transduce the hedgehog's proteins signal. PTCH may function as a tumor suppressor as inactivation of this gene is probably a necessary, if not sufficient step for tumorigenesis. Mutations of the PTCH gene are associated with basal cell nevus syndrome (BCNS), esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly 7 (HPE7). Multiple transcript variants due to alternative splicing have been described but the full-length sequences and biological activity remain undetermined.

 

 

Gene Symbol: PTCH; PTCH1; PTC; BCNS; HPE7; PTC1; NBCCS; PTCH11

 

NCBI Gene ID: 5727

 

Uniprot Entry: Q13635

 

Construct Details: Full length human PTCH gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4344 (bp)

 

Gene Insert Sequence:  

ATGGCCTCGGCTGGTAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGTATCGGTGCCC

CGGGACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGCCGTGCTGCCGCGCCGGACCG

GGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGCTCTGGAGCAGATTTCCAAGGGGAAGGCT

ACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAGAGACTCTTATTTAAACTGGGTTGTTACA

TTCAAAAAAACTGCGGCAAGTTCTTGGTTGTGGGCCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAA

AGCAGCGAACCTCGAGACCAACGTGGAGGAGCTGTGGGTGGAAGTTGGAGGACGAGTAAGTCGTGAATTA

AATTATACTCGCCAGAAGATTGGAGAAGAGGCTATGTTTAATCCTCAACTCATGATACAGACCCCTAAAG

AAGAAGGTGCTAATGTCCTGACCACAGAAGCGCTCCTACAACACCTGGACTCGGCACTCCAGGCCAGCCG

TGTCCATGTATACATGTACAACAGGCAGTGGAAATTGGAACATTTGTGTTACAAATCAGGAGAGCTTATC

ACAGAAACAGGTTACATGGATCAGATAATAGAATATCTTTACCCTTGTTTGATTATTACACCTTTGGACT

GCTTCTGGGAAGGGGCGAAATTACAGTCTGGGACAGCATACCTCCTAGGTAAACCTCCTTTGCGGTGGAC

AAACTTCGACCCTTTGGAATTCCTGGAAGAGTTAAAGAAAATAAACTATCAAGTGGACAGCTGGGAGGAA

ATGCTGAATAAGGCTGAGGTTGGTCATGGTTACATGGACCGCCCCTGCCTCAATCCGGCCGATCCAGACT

GCCCCGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTGATATGGCCCTTGTTTTGAATGGTGGATG

TCATGGCTTATCCAGAAAGTATATGCACTGGCAGGAGGAGTTGATTGTGGGTGGCACAGTCAAGAACAGC

ACTGGAAAACTCGTCAGCGCCCATGCCCTGCAGACCATGTTCCAGTTAATGACTCCCAAGCAAATGTACG

AGCACTTCAAGGGGTACGAGTATGTCTCACACATCAACTGGAACGAGGACAAAGCGGCAGCCATCCTGGA

GGCCTGGCAGAGGACATATGTGGAGGTGGTTCATCAGAGTGTCGCACAGAACTCCACTCAAAAGGTGCTT

TCCTTCACCACCACGACCCTGGACGACATCCTGAAATCCTTCTCTGACGTCAGTGTCATCCGCGTGGCCA

GCGGCTACTTACTCATGCTCGCCTATGCCTGTCTAACCATGCTGCGCTGGGACTGCTCCAAGTCCCAGGG

TGCCGTGGGGCTGGCTGGCGTCCTGCTGGTTGCACTGTCAGTGGCTGCAGGACTGGGCCTGTGCTCATTG

ATCGGAATTTCCTTTAACGCTGCAACAACTCAGGTTTTGCCATTTCTCGCTCTTGGTGTTGGTGTGGATG

ATGTTTTTCTTCTGGCCCACGCCTTCAGTGAAACAGGACAGAATAAAAGAATCCCTTTTGAGGACAGGAC

CGGGGAGTGCCTGAAGCGCACAGGAGCCAGCGTGGCCCTCACGTCCATCAGCAATGTCACAGCCTTCTTC

ATGGCCGCGTTAATCCCAATTCCCGCTCTGCGGGCGTTCTCCCTCCAGGCAGCGGTAGTAGTGGTGTTCA

ATTTTGCCATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCATGGATTTATATCGACGCGAGGACAGGAG

ACTGGATATTTTCTGCTGTTTTACAAGCCCCTGCGTCAGCAGAGTGATTCAGGTTGAACCTCAGGCCTAC

ACCGACACACACGACAATACCCGCTACAGCCCCCCACCTCCCTACAGCAGCCACAGCTTTGCCCATGAAA

CGCAGATTACCATGCAGTCCACTGTCCAGCTCCGCACGGAGTACGACCCCCACACGCACGTGTACTACAC

CACCGCTGAGCCGCGCTCCGAGATCTCTGTGCAGCCCGTCACCGTGACACAGGACACCCTCAGCTGCCAG

AGCCCAGAGAGCACCAGCTCCACAAGGGACCTGCTCTCCCAGTTCTCCGACTCCAGCCTCCACTGCCTCG

AGCCCCCCTGTACGAAGTGGACACTCTCATCTTTTGCTGAGAAGCACTATGCTCCTTTCCTCTTGAAACC

AAAAGCCAAGGTAGTGGTGATCTTCCTTTTTCTGGGCTTGCTGGGGGTCAGCCTTTATGGCACCACCCGA

GTGAGAGACGGGCTGGACCTTACGGACATTGTACCTCGGGAAACCAGAGAATATGACTTTATTGCTGCAC

AATTCAAATACTTTTCTTTCTACAACATGTATATAGTCACCCAGAAAGCAGACTACCCGAATATCCAGCA

CTTACTTTACGACCTACACAGGAGTTTCAGTAACGTGAAGTATGTCATGTTGGAAGAAAACAAACAGCTT

CCCAAAATGTGGCTGCACTACTTCAGAGACTGGCTTCAGGGACTTCAGGATGCATTTGACAGTGACTGGG

AAACCGGGAAAATCATGCCAAACAATTACAAGAATGGATCAGACGATGGAGTCCTTGCCTACAAACTCCT

GGTGCAAACCGGCAGCCGCGATAAGCCCATCGACATCAGCCAGTTGACTAAACAGCGTCTGGTGGATGCA

GATGGCATCATTAATCCCAGCGCTTTCTACATCTACCTGACGGCTTGGGTCAGCAACGACCCCGTCGCGT

ATGCTGCCTCCCAGGCCAACATCCGGCCACACCGACCAGAATGGGTCCACGACAAAGCCGACTACATGCC

TGAAACAAGGCTGAGAATCCCGGCAGCAGAGCCCATCGAGTATGCCCAGTTCCCTTTCTACCTCAACGGC

TTGCGGGACACCTCAGACTTTGTGGAGGCAATTGAAAAAGTAAGGACCATCTGCAGCAACTATACGAGCC

TGGGGCTGTCCAGTTACCCCAACGGCTACCCCTTCCTCTTCTGGGAGCAGTACATCGGCCTCCGCCACTG

GCTGCTGCTGTTCATCAGCGTGGTGTTGGCCTGCACATTCCTCGTGTGCGCTGTCTTCCTTCTGAACCCC

TGGACGGCCGGGATCATTGTGATGGTCCTGGCGCTGATGACGGTCGAGCTGTTCGGCATGATGGGCCTCA

TCGGAATCAAGCTCAGTGCCGTGCCCGTGGTCATCCTGATCGCTTCTGTTGGCATAGGAGTGGAGTTCAC

CGTTCACGTTGCTTTGGCCTTTCTGACGGCCATCGGCGACAAGAACCGCAGGGCTGTGCTTGCCCTGGAG

CACATGTTTGCACCCGTCCTGGATGGCGCCGTGTCCACTCTGCTGGGAGTGCTGATGCTGGCGGGATCTG

AGTTCGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCACCATCCTCGGCGTTCTCAATGG

GCTGGTTTTGCTTCCCGTGCTTTTGTCTTTCTTTGGACCATATCCTGAGGTGTCTCCAGCCAACGGCTTG

AACCGCCTGCCCACACCCTCCCCTGAGCCACCCCCCAGCGTGGTCCGCTTCGCCATGCCGCCCGGCCACA

CGCACAGCGGGTCTGATTCCTCCGACTCGGAGTATAGTTCCCAGACGACAGTGTCAGGCCTCAGCGAGGA

GCTTCGGCACTACGAGGCCCAGCAGGGCGCGGGAGGCCCTGCCCACCAAGTGATCGTGGAAGCCACAGAA

AACCCCGTCTTCGCCCACTCCACTGTGGTCCATCCCGAATCCAGGCATCACCCACCCTCGAACCCGAGAC

AGCAGCCCCACCTGGACTCAGGGTCCCTGCCTCCCGGACGGCAAGGCCAGCAGCCCCGCAGGGACCCCCC

CAGAGAAGGCTTGTGGCCACCCCCCTACAGACCGCGCAGAGACGCTTTTGAAATTTCTACTGAAGGGCAT

TCTGGCCCTAGCAATAGGGCCCGCTGGGGCCCTCGCGGGGCCCGTTCTCACAACCCTCGGAACCCAGCGT

CCACTGCCATGGGCAGCTCCGTGCCCGGCTACTGCCAGCCCATCACCACTGTGACGGCTTCTGCCTCCGT

GACTGTCGCCGTGCACCCGCCGCCTGTCCCTGGGCCTGGGCGGAACCCCCGAGGGGGACTCTGCCCAGGC

TACCCTGAGACTGACCACGGCCTGTTTGAGGACCCCCACGTGCCTTTCCACGTCCGGTGTGAGAGGAGGG

ATTCGAAGGTGGAAGTCATTGAGCTGCAGGACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAGCTCCAA

CTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

1. Science 272:1668-1671(1996) 

2. Cancer Res. 56:4599-4601(1996)

3. J. Biol. Chem. 271:12125-12128(1996)

4. Hum. Genet. 100:497-502(1997)

5. Hum. Mutat. 11:480-480(1998) 

 

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.