Human CD96/TACTILE Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD96, also known as Tactile (T cell-activated increased late expression), is a single pass type I transmembrane protein of the immunoglobulin superfamily (IgSF).  The extracellular domain (ECD) of CD96 contains 2 V-type and 1 C-type Ig-like domains, which are highly glycosylated.  An 80-kDa soluble form of CD96 is elevated in human serum during chronic hepatitis B. CD96 is expressed on activated T, NK and a subpopulation of B cells as well as on acute myeloid leukemia and  myelodysplastic stem cells. CD96 is allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR) or CD155, a nectins and nectin-like protein family member serve to mediate cell-cell adhesion and cell migration. CD96 may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response.  CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells. The most N-terminal Ig-like domain of CD96 binds to PVR.  On NK cells, co-stimulatory molecules CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various members of nectin family.   CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.  CD96 mutations may cause a form of the C syndrome, a set of developmental anomalies in cranial bone, skin and viscera.  

 

 

Gene Symbol: CD96; TACTILE

 

NCBI Gene ID: 10225

 

Uniprot Entry: P40200

 

Construct Details: Full length human CD96 gene (encoding Uniprot#P40200-2) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. 

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGAGAAAAAATGGAAATACTGTGCTGTCTATTACATCATCCAGATACATTTTGTCAAGGGAGTTTGGG

AAAAAACAGTCAACACAGAAGAAAATGTTTATGCTACACTTGGCTCTGATGTCAACCTGACCTGCCAAAC

ACAGACAGTAGGCTTCTTCGTGCAGATGCAATGGTCCAAGGTCACCAATAAGATAGACCTGATTGCTGTC

TATCATCCCCAATACGGCTTCTACTGTGCCTATGGGAGACCCTGTGAGTCACTTGTGACTTTCACAGAAA

CTCCTGAGAATGGGTCAAAATGGACTCTGCACTTAAGGAATATGTCTTGTTCAGTCAGTGGAAGGTACGA

GTGTATGCTTGTTCTGTATCCAGAGGGCATTCAGACTAAAATCTACAACCTTCTCATTCAGACACACGTT

ACAGCAGATGAATGGAACAGCAACCATACGATAGAAATAGAGATAAATCAGACTCTGGAAATACCATGCT

TTCAAAATAGCTCCTCAAAAATTTCATCTGAGTTCACCTATGCATGGTCGGTGGAGGATAATGGAACTCA

GGAAACACTTATCTCCCAAAATCACCTCATCAGCAATTCCACATTACTTAAAGATAGAGTCAAGCTTGGT

ACAGACTACAGACTCCACCTCTCTCCAGTCCAAATCTTCGATGATGGGCGGAAGTTCTCTTGCCACATTA

GAGTCGGTCCTAACAAAATCTTGAGGAGCTCCACCACAGTCAAGGTTTTTGCTAAACCAGAAATCCCTGT

GATTGTGGAAAATAACTCCACGGATGTCTTGGTAGAGAGAAGATTTACCTGCTTACTAAAGAATGTATTT

CCCAAAGCAAATATCACATGGTTTATAGATGGAAGTTTTCTTCATGATGAAAAAGAAGGAATATATATTA

CTAATGAAGAGAGAAAAGGCAAAGATGGATTTTTGGAACTGAAGTCTGTTTTAACAAGGGTACATAGTAA

TAAACCAGCCCAATCAGACAACTTGACCATTTGGTGTATGGCTCTGTCTCCAGTCCCAGGAAATAAAGTG

TGGAACATCTCATCAGAAAAGATCACTTTTCTCTTAGGTTCTGAAATTTCCTCAACAGACCCTCCACTGA

GTGTTACAGAATCTACCCTTGACACCCAACCTTCTCCAGCCAGCAGTGTATCTCCTGCAAGATATCCAGC

TACATCTTCAGTGACCCTTGTAGATGTGAGTGCCTTGAGGCCAAACACCACTCCTCAACCCAGCAATTCC

AGTATGACTACCCGAGGCTTCAACTATCCCTGGACCTCCAGTGGGACAGATACCAAAAAATCAGTTTCAC

GGATACCTAGTGAAACATACAGTTCATCCCCGTCAGGTGCAGGCTCAACACTTCATGACAATGTCTTTAC

CAGCACAGCCAGAGCATTTTCAGAAGTCCCCACAACTGCCAATGGATCTACGAAAACTAATCACGTCCAT

ATCACTGGTATTGTGGTCAATAAGCCCAAAGATGGAATGTCCTGGCCAGTGATTGTAGCAGCTTTACTCT

TTTGCTGCATGATATTGTTTGGTCTTGGAGTGAGAAAATGGTGTCAGTACCAAAAAGAAATAATGGAAAG

ACCTCCACCTTTCAAGCCACCACCACCTCCCATCAAGTACACTTGCATTCAAGAGCCCAACGAAAGTGAT

CTGCCTTATCATGAGATGGAGACCCTCTAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. J. Immunol. 148:2600 (1992)

2. J. Immunol. 172:3994 (2004)

3. Proc. Natl. Acad. Sci. 104:11008 (2007)

4. Am. J. Hum. Genet. 81:835 (2007)

5. J. Biol. Chem. 284:2235 (2009)

 

  

 

 

 

Product datasheet (pdf) can be downloaded here: LTP2473-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.