Human AGRL1/ADGRL1/CIRL1/LEC2/LPHN1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRL1 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRL1 is an orphan GPCR and may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked. It forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). AGRL1 interacts with syntaxin and with proteins of the SHANK family via the PDZ domain and with FLRT1, FLRT2 and FLRT3 via extracellular domain. AGRL1 is a calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. AGRL1 is also a receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. AGRL1 has been implicated in the regulation of exocytosis.
Gene Symbol: AGRL1; ADGRL1; CL1; LEC2; CIRL1; LPHN1; CIRL-1; KIAA0821
NCBI Gene ID: 22859
Uniprot Entry: Q94910
Construct Details: Full length human AGRL1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 4425 (bp)
Gene Insert Sequence:
ATGGCCCGCCTAGCCGCAGTGCTCTGGAATCTGTGTGTCACCGCCGTCCTGGTCACCTCGGCCACCCAAG
GCCTGAGCCGGGCCGGGCTCCCGTTCGGGCTGATGCGCCGGGAGCTGGCGTGTGAAGGCTACCCCATCGA
GCTGCGGTGCCCCGGCAGCGACGTCATCATGGTGGAGAATGCCAACTACGGGCGCACGGACGACAAGATT
TGCGATGCTGACCCTTTCCAGATGGAGAATGTGCAGTGCTACCTGCCGGACGCCTTCAAGATCATGTCAC
AGAGGTGTAACAACCGCACCCAGTGCGTGGTGGTCGCCGGCTCGGATGCCTTTCCTGACCCCTGTCCTGG
GACCTACAAGTACCTGGAGGTGCAGTACGACTGTGTCCCCTACAAAGTGGAGCAGAAAGTCTTCGTGTGC
CCAGGGACCCTGCAGAAGGTGCTGGAGCCCACCTCGACACACGAGTCAGAGCACCAGTCTGGCGCATGGT
GCAAGGACCCGCTGCAGGCGGGTGACCGCATCTACGTGATGCCCTGGATCCCCTACCGCACGGACACACT
GACTGAGTATGCCTCGTGGGAGGACTACGTGGCCGCCCGCCACACCACCACCTACCGCCTGCCCAACCGC
GTGGATGGCACAGGCTTTGTGGTCTACGATGGTGCCGTCTTCTACAACAAGGAGCGCACGCGCAACATCG
TCAAGTATGACCTACGGACGCGCATCAAGAGCGGGGAGACGGTCATCAATACCGCCAACTACCATGACAC
CTCGCCCTACCGCTGGGGCGGAAAGACCGACATTGACCTGGCGGTGGACGAGAACGGGCTGTGGGTCATC
TACGCCACTGAGGGCAACAACGGGCGGCTGGTGGTGAGCCAGCTGAACCCCTACACACTGCGCTTTGAGG
GCACGTGGGAGACGGGTTACGACAAGCGCTCGGCATCCAACGCCTTCATGGTGTGTGGGGTCCTGTACGT
CCTGCGTTCCGTGTACGTGGATGATGACAGCGAGGCGGCTGGCAACCGCGTGGACTATGCCTTCAACACC
AATGCCAACCGCGAGGAGCCTGTCAGCCTCACCTTCCCCAACCCCTACCAGTTCATCTCCTCCGTTGACT
ACAACCCTCGCGACAACCAGCTGTACGTCTGGAACAACTATTTCGTGGTGCGCTACAGCCTGGAGTTCGG
GCCGCCCGACCCCAGTGCTGGCCCAGCCACTTCCCCACCCCTCAGCACGACCACCACAGCCAGGCCCACG
CCCCTCACCAGCACAGCCTCGCCCGCAGCCACCACCCCGCTCCGCCGGGCACCCCTCACCACGCACCCAG
TGGGTGCCATCAACCAGCTGGGACCTGATCTGCCTCCAGCCACAGCCCCAGTCCCCAGCACCCGGCGGCC
CCCAGCCCCGAATCTACACGTGTCCCCTGAGCTCTTCTGCGAGCCCCGAGAGGTACGGCGGGTCCAGTGG
CCGGCCACCCAGCAGGGCATGCTGGTGGAGAGGCCCTGCCCCAAGGGGACTCGAGGAATTGCCTCCTTCC
AGTGTCTACCAGCCTTGGGGCTCTGGAACCCCCGGGGCCCTGACCTCAGCAACTGCACCTCCCCCTGGGT
CAACCAGGTGGCCCAGAAGATCAAGAGTGGGGAGAACGCGGCCAACATCGCCAGCGAGCTGGCCCGACAC
ACCCGGGGCTCCATCTACGCGGGGGACGTCTCCTCCTCTGTGAAGCTGATGGAGCAGCTGCTGGACATCC
TGGATGCCCAGCTGCAGGCCCTGCGGCCCATCGAGCGCGAGTCAGCCGGCAAGAACTACAACAAGATGCA
CAAGCGAGAGAGAACTTGTAAGGATTATATCAAGGCCGTGGTGGAGACAGTGGACAATCTGCTCCGGCCA
GAAGCTCTGGAGTCCTGGAAGGACATGAATGCCACGGAGCAGGTGCACACGGCCACCATGCTCCTCGACG
TCCTGGAGGAGGGCGCCTTCCTGCTGGCCGACAATGTCAGGGAGCCTGCCCGCTTCCTGGCTGCCAAGGA
GAACGTGGTCCTGGAGGTCACAGTCCTGAACACAGAGGGCCAGGTGCAGGAGCTGGTGTTCCCCCAGGAG
GAGTACCCGAGAAAGAACTCCATCCAGCTGTCTGCCAAAACCATCAAGCAGAACAGCCGCAATGGGGTGG
TCAAAGTTGTCTTCATCCTCTACAACAACCTGGGCCTCTTCCTGTCCACGGAGAATGCCACAGTGAAGCT
GGCCGGCGAAGCAGGCCCGGGTGGCCCTGGGGGCGCCTCTCTAGTGGTGAACTCACAGGTCATCGCAGCA
TCCATCAACAAGGAGTCCAGCCGCGTCTTCCTCATGGACCCTGTCATCTTCACCGTGGCCCACCTGGAGG
ACAAGAACCACTTCAATGCTAACTGCTCCTTCTGGAACTACTCGGAGCGTTCCATGCTGGGCTACTGGTC
GACCCAAGGCTGCCGCCTGGTGGAGTCCAACAAGACCCATACCACGTGTGCCTGCAGCCACCTCACCAAC
TTCGCTGTGCTCATGGCTCACCGTGAGATCTACCAGGGCCGCATCAACGAGCTGCTGCTGTCGGTCATCA
CCTGGGTGGGCATTGTGATCTCCCTGGTCTGCTTGGCCATCTGCATCTCCACCTTCTGCTTCCTGCGGGG
GCTGCAGACCGACCGCAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTGGCTGAGCTGCTCTTC
CTGGTCGGGATCGACAAGACTCAGTATGAGATTGCCTGCCCCATCTTCGCCGGCCTGCTGCACTATTTCT
TCCTGGCTGCCTTCTCCTGGCTGTGCCTGGAGGGCGTGCACCTCTACCTGCTACTAGTGGAGGTGTTTGA
GAGCGAGTATTCCCGCACCAAGTACTACTACCTGGGTGGCTACTGCTTCCCGGCCCTGGTGGTGGGCATC
GCGGCTGCCATTGACTACCGCAGCTACGGCACCGAGAAGGCCTGCTGGCTCCGAGTGGACAATTACTTCA
TCTGGAGTTTCATCGGGCCAGTCTCCTTCGTTATCGTGGTCAACCTGGTGTTCCTCATGGTGACCCTGCA
CAAGATGATCCGAAGCTCATCTGTGCTCAAGCCCGACTCCAGCCGCCTGGACAACATTAAATCCTGGGCG
CTGGGGGCCATCGCGCTGCTGTTCCTGCTGGGCCTCACCTGGGCTTTCGGCCTCCTCTTCATCAACAAGG
AGTCGGTGGTCATGGCCTATCTCTTCACCACCTTCAACGCCTTCCAGGGGGTCTTCATCTTCGTCTTTCA
CTGCGCCTTACAGAAGAAGGTGCACAAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATCCGC
TCCCCACCCGGGGGCACTCACGGATCCCTCAAGACCTCAGCCATGCGAAGCAACACCCGCTACTACACAG
GGACCCAGAGCCGAATTCGGAGGATGTGGAATGACACTGTGAGGAAACAGACGGAGTCCTCCTTCATGGC
GGGTGACATCAACAGCACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTGCTGACCAACCCCGTG
CTGCAGCCCCGTGGGGGCACCAGTCCCTACAACACCCTCATCGCCGAGTCAGTGGGCTTCAATCCCTCCT
CGCCCCCTGTCTTCAACTCCCCAGGGAGCTACCGGGAACCCAAGCACCCCTTGGGAGGCCGGGAAGCCTG
TGGCATGGACACCCTGCCCCTGAACGGCAACTTCAATAACAGTTACTCCTTGCGAAGTGGGGATTTCCCT
CCCGGGGATGGGGGCCCTGAGCCGCCCCGAGGCCGGAACCTAGCCGATGCGGCGGCCTTTGAGAAGATGA
TCATCTCAGAGCTGGTGCACAACAACCTGCGGGGGAGCAGCAGCGCGGCCAAGGGCCCTCCACCGCCTGA
GCCCCCTGTGCCACCTGTGCCAGGGGGCGGGGGCGAGGAAGAGGCGGGCGGGCCCGGGGGTGCTGACCGG
GCCGAGATTGAACTTCTCTATAAGGCCCTGGAGGAGCCTCTGCTGCTGCCCCGGGCCCAGTCGGTGCTGT
ACCAGAGCGATCTGGACGAGTCGGAGAGCTGCACGGCCGAGGACGGCGCCACCAGCCGGCCCCTCTCCTC
CCCTCCTGGCCGGGACTCCCTCTATGCCAGCGGGGCCAACCTGCGGGACTCACCCTCCTACCCGGACAGC
AGCCCTGAGGGGCCCAGTGAGGCCCTGCCCCCACCCCCTCCCGCACCCCCCGGCCCCCCCGAAATCTACT
ACACCTCGCGCCCGCCAGCCCTGGTGGCCCGGAATCCCCTGCAGGGCTACTACCAGGTGCGGCGTCCTAG
CCACGAGGGCTACCTGGCAGCCCCAGGCCTTGAGGGGCCAGGGCCCGATGGGGACGGGCAGATGCAGCTG
GTCACCAGTCTCTGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.