Human AGRG7/ADGRG7/GPR128 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRG7 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRG7 is an orphan GPCR and may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked.
Gene Symbol: AGRG7; ADGRG7; GPR128
NCBI Gene ID: 84873
Uniprot Entry: Q96K78
Construct Details: Full length human AGRG7 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2394 (bp)
Gene Insert Sequence:
ATGGCTTCCTGCCGTGCCTGGAACCTTAGGGTGCTGGTGGCTGTCGTGTGTGGACTACTGACTGGCATCA
TTTTGGGACTGGGCATCTGGAGGATTGTGATCAGGATCCAAAGAGGAAAATCTACTTCCTCATCAAGCAC
CCCTACAGAGTTCTGCAGGAATGGTGGAACCTGGGAAAATGGCAGATGTATTTGTACAGAAGAGTGGAAA
GGACTGAGATGTACAATTGCTAATTTTTGTGAAAATAGTACCTATATGGGTTTTACTTTTGCCAGAATCC
CAGTGGGCAGATATGGACCATCCTTGCAAACATGTGGCAAGGATACTCCAAATGCGGGCAATCCAATGGC
AGTCCGGTTGTGCAGTCTCTCTCTATATGGAGAGATAGAATTACAAAAAGTGACAATAGGAAATTGCAAT
GAAAATCTGGAAACCCTGGAAAAGCAGGTAAAGGATGTCACAGCACCACTTAATAACATTTCTTCTGAAG
TCCAGATTTTAACATCTGATGCCAATAAATTAACTGCTGAGAACATCACTAGTGCTACGCGAGTGGTTGG
ACAGATATTCAACACTTCCAGAAATGCTTCACCTGAGGCAAAGAAAGTTGCCATAGTAACAGTGAGTCAA
CTCCTAGATGCCAGTGAAGATGCTTTTCAAAGAGTTGCTGCTACTGCTAATGATGATGCCCTTACAACGC
TTATTGAGCAAATGGAGACTTATTCCTTGTCTTTGGGTAATCAATCAGTGGTGGAACCTAACATAGCAAT
ACAGTCAGCAAATTTCTCTTCAGAAAATGCGGTGGGGCCTTCAAATGTTCGCTTCTCTGTGCAGAAAGGA
GCTAGCAGTTCTCTAGTTTCTAGTTCAACATTTATACATACAAATGTGGATGGCCTTAACCCAGATGCAC
AGACTGAGCTTCAGGTCTTGCTTAATATGACGAAAAATTACACCAAGACATGCGGCTTTGTAGTTTATCA
AAATGACAAGCTTTTCCAATCAAAAACTTTTACAGCTAAATCGGATTTTAGTCAAAAAATTATCTCAAGC
AAAACTGATGAAAATGAGCAAGATCAGAGTGCTTCTGTTGACATGGTCTTTAGTCCAAAGTACAACCAAA
AAGAATTTCAACTCTATTCCTATGCCTGTGTCTATTGGAATTTGTCAGCGAAGGACTGGGACACATATGG
CTGTCAAAAAGACAAGGGCACTGATGGATTCCTGCGCTGCCGCTGCAACCATACTACTAATTTTGCTGTA
TTAATGACTTTCAAAAAGGATTATCAATATCCCAAATCACTTGACATATTATCCAACGTTGGATGTGCAC
TGTCTGTTACTGGTCTGGCTCTCACAGTTATATTTCAGATTGTCACCAGGAAAGTCAGAAAAACCTCAGT
AACCTGGGTTTTGGTCAATCTGTGCATATCAATGTTGATTTTCAACCTCCTCTTTGTGTTTGGAATTGAA
AACTCCAATAAGAACTTGCAGACAAGTGATGGTGACATCAATAATATTGACTTTGACAATAATGACATAC
CCAGGACAGACACCATTAACATCCCGAATCCCATGTGCACTGCGATTGCCGCCTTACTGCACTATTTTCT
GTTAGTGACATTTACCTGGAACGCACTCAGCGCTGCACAGCTCTATTACCTTCTAATAAGGACCATGAAG
CCTCTTCCTCGGCATTTCATTCTTTTCATCTCATTAATTGGATGGGGAGTCCCAGCTATAGTAGTGGCTA
TAACAGTGGGAGTTATTTATTCTCAGAATGGAAATAATCCACAGTGGGAATTAGACTACCGGCAAGAGAA
AATCTGCTGGCTGGCAATTCCAGAACCCAATGGTGTTATAAAAAGTCCGCTGTTGTGGTCATTCATCGTA
CCTGTAACCATTATCCTCATCAGCAATGTTGTTATGTTTATTACAATCTCGATCAAAGTGCTGTGGAAGA
ATAACCAGAACCTGACAAGCACAAAAAAAGTTTCATCCATGAAGAAGATTGTTAGCACATTATCTGTTGC
AGTTGTTTTTGGAATTACCTGGATTCTAGCATACCTGATGCTAGTTAATGATGATAGCATCAGGATCGTC
TTCAGCTACATATTCTGCCTTTTCAACACTACACAGGGATTGCAAATTTTTATCCTGTACACTGTTAGAA
CAAAAGTCTTCCAGAGTGAAGCTTCCAAAGTGTTGATGTTGCTATCGTCTATTGGGAGAAGGAAGTCATT
GCCTTCAGTGACGCGGCCGAGGCTGCGTGTAAAGATGTATAATTTCCTCAGGTCATTGCCAACCTTACAT
GAACGCTTTAGGCTACTGGAAACCTCTCCGAGTACTGAGGAAATCACACTCTCTGAAAGTGACAATGCAA
AGGAAAGCATCTAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.