Human AGRE3/ADGRE3/EMR3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRE3 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. ADRE3 is characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a 7TM domain via a mucin-like spacer domain. AGRE3 may be cleaved into 2 subunits, an extracellular alpha subunit and a 7TM beta subunit. The AGRE3 gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. AGRE3 may play a role in myeloid-myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of momocytes-derived macrophages and activated neutrophils.
Gene Symbol: AGRE3; ADGRE3; EMR3; UNQ683/PRO1562
NCBI Gene ID: 84658
Uniprot Entry: Q9BY15
Construct Details: Full length human AGRE3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 1959 (bp)
Gene Insert Sequence:
ATGCAGGGACCATTGCTTCTTCCAGGCCTCTGCTTTCTGCTGAGCCTCTTTGGAGCTGTGACTCAGAAAA
CCAAAACTTCCTGTGCTAAGTGCCCCCCAAATGCTTCCTGTGTCAATAACACTCACTGCACCTGCAACCA
TGGATATACTTCTGGATCTGGGCAGAAACTATTCACATTCCCCTTGGAGACATGTAACGACATTAATGAA
TGTACACCACCCTATAGTGTATATTGTGGATTTAACGCTGTGTGTTACAATGTCGAAGGAAGTTTCTACT
GTCAATGTGTCCCAGGATATAGACTGCATTCTGGGAATGAACAATTCAGTAATTCCAATGAGAACACCTG
TCAGGACACCACCTCCTCAAAGACAACCGAGGGCAGGAAAGAGCTGCAAAAGATTGTGGACAAATTTGAG
TCACTTCTCACCAATCAGACTTTATGGAGAACAGAAGGGAGACAAGAAATCTCATCCACAGCTACCACTA
TTCTCCGGGATGTGGAATCGAAAGTTCTAGAAACTGCCTTGAAAGATCCAGAACAAAAAGTCCTGAAAAT
CCAAAACGATAGTGTAGCTATTGAAACTCAAGCGATTACAGACAATTGCTCTGAAGAAAGAAAGACATTC
AACTTGAACGTCCAAATGAACTCAATGGACATCCGTTGCAGTGACATCATCCAGGGAGACACACAAGGTC
CCAGTGCCATTGCCTTTATCTCATATTCTTCTCTTGGAAACATCATAAATGCAACTTTTTTTGAAGAGAT
GGATAAGAAAGATCAAGTGTATCTGAACTCTCAGGTTGTGAGTGCTGCTATTGGACCCAAAAGGAACGTG
TCTCTCTCCAAGTCTGTGACGCTGACTTTCCAGCACGTGAAGATGACCCCCAGTACCAAAAAGGTCTTCT
GTGTCTACTGGAAGAGCACAGGGCAGGGCAGCCAGTGGTCCAGGGATGGCTGCTTCCTGATACACGTGAA
CAAGAGTCACACCATGTGTAATTGCAGTCACCTGTCCAGCTTCGCTGTCCTGATGGCCCTGACCAGCCAG
GAGGAGGATCCCGTGCTGACTGTCATCACCTACGTGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGG
CGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCGGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCT
CTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGGGATTGATCGAACTGAACCCAAGGTGCTGTGCTCC
ATCATCGCCGGTGCTTTGCACTATCTCTACCTGGCCGCCTTCACCTGGATGCTGCTGGAGGGTGTGCACC
TCTTCCTCACTGCACGGAACCTGACAGTGGTCAACTACTCAAGCATCAATAGACTCATGAAGTGGATCAT
GTTCCCAGTCGGCTATGGCGTTCCCGCTGTGACTGTGGCCATTTCTGCAGCCTCCTGGCCTCACCTTTAT
GGAACTGCTGATCGATGCTGGCTCCACCTGGACCAGGGATTCATGTGGAGTTTCCTTGGCCCAGTCTGTG
CCATTTTCTCTGCGAATTTAGTATTGTTTATCTTGGTCTTTTGGATTTTGAAAAGAAAACTTTCCTCCCT
CAATAGTGAAGTGTCAACCATCCAGAACACAAGGATGCTGGCTTTCAAAGCAACAGCTCAGCTCTTCATC
CTGGGCTGCACATGGTGTCTGGGCTTGCTACAGGTGGGTCCAGCTGCCCAGGTCATGGCCTACCTCTTCA
CCATCATCAACAGCCTCCAAGGCTTCTTCATCTTCTTGGTCTACTGCCTCCTCAGCCAGCAGGTCCAGAA
ACAATATCAAAAGTGGTTTAGAGAGATCGTAAAATCAAAATCTGAGTCTGAGACATACACACTTTCCAGC
AAGATGGGTCCTGACTCAAAACCCAGTGAGGGGGATGTTTTTCCAGGACAAGTGAAGAGAAAATATTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.