Human AGRE2/CD312/ADGRE2/EMR2 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRE2 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. ADRE2 is characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a 7TM domain via a mucin-like spacer domain. AGRE2 may be autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a 7TM beta subunit. ADRE2 is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with the chondroitin sulfate moiety of glycosaminoglycan chains. ADRE2 is thought to promote granulocyte chemotaxis, degranulation and adhesion. In macrophages, it induces the release of inflammatory cytokines, including IL8 and TNF.
Gene Symbol: AGRE2; CD312; ADGRE2; EMR2
NCBI Gene ID: 30817
Uniprot Entry: Q9UHX3
Construct Details: Full length human AGRE2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2472 (bp)
Gene Insert Sequence:
ATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCC
AGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTG
CAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAG
TGTGCAACACTGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACT
GCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACGTG
TCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTC
GGCAGCTACACGTGCCAGTGCCTGCCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGCACAGATG
TGAATGAATGCACCTCCGGACAAAACCCATGCCACAGCTCCACCCACTGCCTCAACAACGTGGGCAGCTA
TCAGTGCCGCTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGT
GAAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGG
GTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACAC
TGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGA
TTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCA
TCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCA
CTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAAT
GGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGA
GTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCC
AGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTC
CTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCC
TGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTT
CACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAAT
GGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTT
GCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGAC
TGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTG
TGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACC
TCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCA
CTATCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAAC
CTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAG
TCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTG
GCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTA
GTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCC
TCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCT
GGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAG
GGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCA
AAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTC
CAAACCCAGCACGGTTAACTAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.