Human AGRE1/ADGRE1/EMR1/TM7LN3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRE1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRE1 is an orphan GPCR with 6 EGF-like domains involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. AGRE1 may play a role in regulatory T-cells (Treg) development and function. AGRE1 contains 7 transmembrane (7TM) domains at its C-terminus. The N-terminus of the encoded protein has 6 EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties.
Gene Symbol: AGRE1; ADGRE1; EMR1; TM7LN3
NCBI Gene ID: 2015
Uniprot Entry: Q14246
Construct Details: Full length human AGRE1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2661 (bp)
Gene Insert Sequence:
ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGAC
CCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACCTG
CACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAATCAC
TTCAAGGATCCAGGAGTGCGATGCAAAGATATTGATGAATGTTCTCAAAGCCCCCAGCCCTGTGGTCCTA
ACTCATCCTGCAAAAACCTGTCAGGGAGGTACAAGTGCAGCTGTTTAGATGGTTTCTCTTCTCCCACTGG
AAATGACTGGGTCCCAGGAAAGCCGGGCAATTTCTCCTGTACTGATATCAATGAGTGCCTCACCAGCAGC
GTCTGCCCTGAGCATTCTGACTGTGTCAACTCCATGGGAAGCTACAGTTGCAGCTGTCAAGTTGGATTCA
TCTCTAGAAACTCCACCTGTGAAGACGTGGATGAATGTGCAGATCCAAGAGCTTGCCCAGAGCATGCAAC
TTGTAATAACACTGTTGGAAACTACTCTTGTTTCTGCAACCCAGGATTTGAATCCAGCAGTGGCCACTTG
AGTTTCCAGGGTCTCAAAGCATCGTGTGAAGATATTGATGAATGCACTGAAATGTGCCCCATCAATTCAA
CATGCACCAACACTCCTGGGAGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGCAATGGACAGTT
GAATTTCACAGACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCATCAACCTGTGGT
CCTAATTCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGCTTTCATCCCAATC
CAGAAGGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTCAAATGTAAGGAAGATGTGAT
ACCCGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCGCAGTGAAACCTGCATATGTCTCCTTTTGT
GCACAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTGTGAAAATAAAACGACCGTAGTTTCTCTGA
AGAATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATATCCACGTGGACTAAATTCACCAAGGAAGA
GACGTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTGGCATCTTTTTGGAAACCC
TCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGCAAAGTTATCAACAAAGAAT
GCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATGAAGATCGGGTGTTCCACAAT
TGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTGTCTCCTTTGTGGGCATGGAATCGGTTTTA
AATGAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCACCTCTGAGATCAAGCTGAAGATGAATTCTC
GAGTCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGCTTCTCAGATCCAATCATCTACACTCTGGA
GAACATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCCTGGAGCACTGATGTGAAGGGTGGA
AGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACATATACCATCTGCAGCTGTAATCAGA
TGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATGGACTTTTCCTTGTACATCATTAGCCA
TGTAGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCCATCGCCACCTTTCTGCTGTGTCGCTCCATC
CGAAATCACAACACCTACCTCCACCTGCACCTCTGCGTGTGTCTCCTCTTGGCGAAGACTCTCTTCCTCG
CCGGTATACACAAGACTGACAACAAGATGGGCTGCGCCATCATCGCGGGCTTCCTGCACTACCTTTTCCT
TGCCTGCTTCTTCTGGATGCTGGTGGAGGCTGTGATACTGTTCTTGATGGTCAGAAACCTGAAGGTGGTG
AATTACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTGTGCCTTTGGTTATGGGCTGCCGATGCTGG
TGGTGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGAATGCATAATCGCTGCTGGCTGAATACAGA
GACAGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACAGTTATAGTGATCAACTCCCTTCTCCTGACC
TGGACCTTGTGGATCCTGAGGCAGAGGCTTTCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCA
GGTTACTGACCTTCAAGGCCTTTGCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCA
GATTGGACCTGTGGCAGGTGTCATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATC
TTCCTCATCCACTGTCTGCTCAACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACTGGGAAGACGA
AGCCCAGCTCCCAGTCCCAGACCTCAAGGATCTTGCTGTCCTCCATGCCATCCGCTTCCAAGACGGGTTA
A
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.