Human BAI3/AGRB3/ADGRB3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
BAI2 or AGRB2 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways.AGRB2 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions. AGRB2 may be a brain-specific angiogenesis inhibitor (also known as BAI2). The mature peptide may be further cleaved into additional products. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis.
Gene Symbol: AGRB3; ADGRB3; BAI3; KIAA0550
NCBI Gene ID: 577
Uniprot Entry: O60242
Construct Details: Full length human BAI3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 4569 (bp)
Gene Insert Sequence:
ATGAAGGCTGTTCGTAACCTGCTGATTTATATATTTTCCACCTATCTCCTGGTTATGTTTGGATTTAATG
CTGCCCAAGACTTCTGGTGTTCAACTTTGGTGAAGGGAGTCATTTATGGATCGTATTCTGTAAGTGAAAT
GTTTCCTAAAAACTTTACAAACTGCACTTGGACGCTGGAAAATCCAGATCCAACCAAATATAGCATTTAC
CTGAAATTTTCCAAAAAGGACCTTAGCTGCTCTAACTTTTCACTCCTGGCTTATCAGTTTGATCATTTTT
CCCATGAAAAAATAAAGGATCTTTTAAGAAAGAATCATTCTATAATGCAACTCTGCAATTCCAAGAATGC
TTTCGTTTTTCTACAGTATGATAAAAATTTTATTCAAATACGTCGAGTATTTCCAACTAATTTCCCAGGA
TTACAGAAAAAAGGGGAAGAAGATCAGAAATCTTTTTTTGAGTTTTTGGTATTGAACAAGGTCAGCCCAA
GCCAGTTTGGTTGCCATGTATTATGTACTTGGTTGGAGAGCTGCTTAAAATCAGAAAATGGGAGAACAGA
ATCATGTGGGATCATGTATACAAAATGCACCTGCCCTCAGCATTTGGGAGAGTGGGGGATCGACGACCAG
TCGCTGATTTTGTTAAATAACGTGGTGTTACCCCTGAATGAGCAGACAGAGGGCTGCCTGACCCAGGAGC
TGCAAACCACCCAAGTCTGCAATCTTACCAGGGAGGCCAAGCGACCACCCAAAGAAGAATTTGGAATGAT
GGGAGATCATACAATTAAAAGTCAGCGACCTCGATCTGTTCATGAAAAAAGGGTCCCTCAGGAACAAGCT
GATGCTGCTAAATTTATGGCACAAACTGGTGAATCTGGTGTGGAAGAGTGGTCCCAGTGGAGCACATGTT
CGGTTACTTGTGGTCAAGGGTCGCAGGTGCGAACCAGAACTTGTGTATCACCTTACGGGACACACTGCAG
CGGCCCATTAAGAGAATCAAGGGTTTGCAATAACACTGCCCTCTGTCCAGTACACGGAGTATGGGAGGAA
TGGTCACCATGGAGTTTATGTTCATTTACATGTGGTCGAGGCCAAAGAACAAGAACAAGGTCATGCACAC
CTCCTCAGTATGGAGGAAGGCCGTGTGAAGGACCTGAAACACATCATAAGCCTTGTAATATTGCTCTTTG
CCCAGTTGATGGACAGTGGCAAGAGTGGAGTTCGTGGAGCCAGTGCTCAGTAACGTGCTCGAATGGGACT
CAGCAGAGAAGCCGGCAGTGCACTGCAGCTGCCCATGGAGGCTCCGAATGCAGAGGGCCATGGGCAGAAA
GCAGAGAGTGCTATAACCCTGAATGTACAGCCAATGGTCAATGGAATCAGTGGGGTCATTGGAGTGGTTG
TTCCAAGTCCTGTGATGGCGGCTGGGAAAGGCGAATAAGGACCTGTCAGGGTGCAGTGATAACAGGGCAG
CAATGTGAAGGAACGGGCGAAGAAGTGAGAAGATGCAATGAGCAGCGATGCCCTGCACCTTATGAAATAT
GCCCTGAGGATTATCTGATGTCGATGGTGTGGAAAAGAACTCCAGCAGGCGACTTGGCATTCAATCAATG
TCCCCTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTTCTGGGAA
CAGCCGAGCTTTGCAAGATGCATATCAAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTG
CTAAGGGGCAGCGAATGCTGGCAGGTGATGGAATGTCCCAGGTGACCAAGACACTGTTGGATTTAACTCA
GAGAAAAAATTTCTATGCAGGCGATCTTCTGATGTCTGTGGAGATCCTGAGAAATGTGACAGACACATTT
AAAAGGGCAAGTTACATCCCTGCATCTGATGGTGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTAG
ATGAAGAAAACAAGGAAAAATGGGAAGATGCACAACAGATTTATCCAGGGTCAATAGAGTTAATGCAGGT
GATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAGAATTCATACTTAATGACTGGA
AATGTAGTGGCTAGTATTCAGAAGCTTCCTGCAGCCTCTGTTCTAACAGACATCAACTTTCCAATGAAAG
GACGGAAGGGAATGGTTGACTGGGCAAGAAACTCAGAAGATAGGGTAGTAATTCCAAAAAGCATTTTCAC
TCCGGTGTCATCAAAAGAATTAGATGAATCATCTGTATTTGTTCTTGGCGCAGTCCTATACAAAAACTTA
GATCTAATTTTGCCCACTTTGAGAAATTATACTGTCATTAATTCCAAAATCATCGTGGTCACAATAAGGC
CTGAACCCAAAACAACCGATTCGTTTCTGGAGATAGAACTAGCTCATTTGGCTAATGGTACTTTGAATCC
CTATTGTGTATTGTGGGATGACTCCAAAACGAACGAGTCTTTGGGAACGTGGTCCACCCAGGGATGTAAA
ACTGTGCTTACCGATGCATCCCATACGAAATGCTTATGTGATCGTCTCTCTACCTTCGCCATTTTGGCTC
AGCAACCTAGAGAAATAATCATGGAATCCTCTGGCACACCTTCAGTTACCCTAATAGTAGGCAGTGGTCT
TTCTTGCTTGGCCTTGATTACCCTAGCAGTTGTCTATGCAGCATTATGGAGGTACATACGCTCTGAGAGA
TCCATAATACTAATTAACTTCTGCCTGTCTATCATCTCATCCAATATCCTCATACTGGTTGGACAGACTC
AGACACATAATAAGAGTATCTGCACAACCACCACTGCATTTTTGCACTTTTTCTTCCTGGCTTCATTCTG
TTGGGTTTTGACTGAGGCGTGGCAATCATATATGGCTGTAACTGGAAAAATTAGGACACGGCTTATAAGA
AAACGCTTTTTGTGCCTTGGATGGGGTTTACCAGCATTAGTAGTGGCCACATCAGTAGGCTTCACCAGAA
CAAAAGGATATGGCACTGATCACTACTGCTGGCTCTCTCTTGAAGGAGGACTACTCTATGCTTTTGTGGG
ACCTGCAGCCGCTGTTGTCCTGGTCAACATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGA
GATGGAATCCTAGATAAAAAGCTCAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTTTGACGC
TCAAATGTGCCAAGTGTGGAGTAGTTTCAACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGC
GTCTCTTTGGAGCTCCTGTGTGGTGTTGCCCCTTCTGGCTTTGACGTGGATGTCTGCGGTTCTGGCCATG
ACAGATAAACGCTCCATATTGTTTCAAATACTTTTTGCTGTGTTTGATTCATTGCAAGGCTTTGTTATAG
TCATGGTCCACTGCATTCTTCGGAGAGAGGTTCAGGATGCATTTAGATGCCGATTGAGAAACTGTCAGGA
TCCCATCAATGCAGATTCTTCGAGTTCGTTTCCTAATGGGCATGCTCAAATCATGACAGACTTTGAAAAG
GATGTAGACATTGCCTGTCGATCAGTTCTTCATAAGGATATTGGTCCTTGCCGAGCAGCCACAATAACAG
GAACACTTTCTAGGATTTCTCTAAATGATGATGAAGAAGAAAAGGGAACAAACCCTGAAGGGCTAAGCTA
TTCAACATTGCCTGGAAATGTCATTTCCAAAGTCATCATCCAGCAACCCACAGGTTTGCACATGCCCATG
AGTATGAATGAGCTTAGCAATCCATGTTTGAAAAAAGAAAATAGTGAATTGCGGAGAACTGTGTACTTAT
GTACGGATGATAATTTGAGAGGGGCTGACATGGACATAGTCCATCCTCAAGAAAGAATGATGGAAAGTGA
CTATATTGTGATGCCCAGAAGTTCTGTAAATAACCAGCCTTCAATGAAAGAAGAAAGCAAAATGAATATT
GGCATGGAAACCTTGCCGCATGAAAGGCTATTGCACTACAAAGTAAACCCTGAATTCAATATGAATCCCC
CTGTAATGGACCAGTTCAATATGAACTTAGAGCAACATCTCGCACCCCAGGAACATATGCAGAATTTGCC
CTTTGAACCTCGCACAGCTGTGAAGAATTTCATGGCCTCTGAGTTGGATGATAATGCAGGACTATCAAGA
AGTGAAACTGGATCAACGATATCAATGAGTTCTTTAGAGAGAAGAAAATCACGATATTCAGACCTTGACT
TTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACTATTTCAAGAACTAAATCAGAAATTTCAAAC
TTTGGACAGATTTCGGGATATACCAAATACAAGCAGTATGGAAAACCCCGCACCAAACAAGAATCCATGG
GACACTTTCAAAAACCCCAGTGAATACCCGCATTACACCACAATCAATGTCTTAGACACAGAGGCAAAGG
ATGCTTTGGAACTGAGGCCAGCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAGAGGGTGA
CTTTCAAACAGAAGTTTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.