Human AGRA3/ADGRA3/GPR125/PGR21/TEM5L Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
AGRA3 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRA3 may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia.
Gene Symbol: AGRA3; ADGRA3; PGR21; TEM5L; GPR125; UNQ556/PRO1113
NCBI Gene ID: 166647
Uniprot Entry: Q8IWK6
Construct Details: Full length human AGRA3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 3966 (bp)
Gene Insert Sequence:
ATGGAGCCACCCGGACGCCGGCGGGGCCGCGCGCAGCCGCCGCTGTTGCTGCCGCTCTCGCTGTTAGCGC
TGCTCGCGCTGCTGGGAGGCGGCGGCGGCGGCGGCGCCGCGGCGCTGCCCGCCGGCTGCAAGCACGATGG
GCGGCCCCGAGGGGCTGGCAGGGCGGCGGGCGCCGCCGAGGGCAAGGTGGTGTGCAGCAGCCTGGAACTC
GCGCAGGTCCTGCCCCCAGATACTCTGCCCAACCGCACGGTCACCCTGATTCTGAGTAACAATAAGATAT
CCGAGCTGAAGAATGGCTCATTTTCTGGGTTAAGTCTCCTTGAAAGATTGGACCTCCGAAACAATCTTAT
TAGTAGTATAGATCCAGGTGCCTTCTGGGGACTGTCATCTCTAAAAAGATTGGATCTGACAAACAATCGA
ATAGGATGTCTGAATGCAGACATATTTCGAGGACTCACCAATCTGGTTCGGCTAAACCTTTCGGGGAATT
TGTTTTCTTCATTATCTCAAGGAACTTTTGATTATCTTGCGTCATTACGGTCTTTGGAATTCCAGACTGA
GTATCTTTTGTGTGACTGTAACATACTGTGGATGCATCGCTGGGTAAAGGAGAAGAACATCACGGTACGG
GATACCAGGTGTGTTTATCCTAAGTCACTGCAGGCCCAACCAGTCACAGGCGTGAAGCAGGAGCTGTTGA
CATGCGACCCTCCGCTTGAATTGCCGTCTTTCTACATGACTCCATCTCATCGCCAAGTTGTGTTTGAAGG
AGACAGCCTTCCTTTCCAGTGCATGGCTTCATATATTGATCAGGACATGCAAGTGTTGTGGTATCAGGAT
GGGAGAATAGTTGAAACCGATGAATCGCAAGGTATTTTTGTTGAAAAGAACATGATTCACAACTGCTCCT
TGATTGCAAGTGCCCTAACCATTTCTAATATTCAGGCTGGATCTACTGGAAATTGGGGCTGTCATGTCCA
GACCAAACGTGGGAATAATACGAGGACTGTGGATATTGTGGTATTAGAGAGTTCTGCACAGTACTGTCCT
CCAGAGAGGGTGGTAAACAACAAAGGTGACTTCAGATGGCCCAGAACATTGGCAGGCATTACTGCATATC
TGCAGTGTACGCGGAACACCCATGGCAGTGGGATATATCCCGGAAACCCACAGGATGAGAGAAAAGCTTG
GCGCAGATGTGATAGAGGTGGCTTTTGGGCAGATGATGATTATTCTCGCTGTCAGTATGCAAATGATGTC
ACTAGAGTTCTTTATATGTTTAATCAGATGCCCCTCAATCTTACCAATGCCGTGGCAACAGCTCGACAGT
TACTGGCTTACACTGTGGAAGCAGCCAACTTTTCTGACAAAATGGATGTTATATTTGTGGCAGAAATGAT
TGAAAAATTTGGAAGATTTACCAAGGAGGAAAAATCAAAAGAGCTAGGTGACGTGATGGTTGACATTGCA
AGTAACATCATGTTGGCTGATGAACGTGTCCTGTGGCTGGCGCAGAGGGAAGCTAAAGCCTGCAGTAGGA
TTGTGCAGTGTCTTCAGCGCATTGCTACCTACCGGCTAGCCGGTGGAGCTCACGTTTATTCAACATATTC
ACCCAATATTGCTCTGGAAGCTTATGTCATCAAGTCTACTGGCTTCACGGGGATGACCTGTACCGTGTTC
CAGAAAGTGGCAGCCTCTGATCGTACAGGACTTTCGGATTATGGGAGGCGGGATCCAGAGGGAAACCTGG
ATAAGCAGCTGAGCTTTAAGTGCAATGTTTCAAATACATTTTCGAGTCTGGCACTAAAGAATACTATTGT
GGAGGCTTCTATTCAGCTTCCTCCTTCCCTTTTCTCACCAAAGCAAAAAAGAGAACTCAGACCAACTGAT
GACTCTCTTTACAAGCTTCAACTCATTGCATTCCGCAATGGAAAGCTTTTTCCAGCCACTGGAAATTCAA
CAAATTTGGCTGATGATGGAAAACGACGTACTGTGGTTACCCCTGTGATTCTCACCAAAATAGATGGTGT
GAATGTAGATACCCACCACATCCCTGTTAATGTGACACTGCGTCGAATTGCACATGGAGCAGATGCTGTT
GCAGCCCGGTGGGATTTCGATTTGCTGAACGGACAAGGAGGCTGGAAGTCAGATGGGTGCCATATACTCT
ATTCAGATGAAAATATCACTACGATTCAGTGCTACTCCCTTAGTAACTATGCAGTTTTAATGGATTTGAC
GGGATCTGAACTATACACCCAGGCGGCCAGCCTCCTGCATCCTGTGGTTTATACTACCGCTATCATTCTC
CTCTTATGTCTCTTAGCCGTCATTGTCAGTTACATATACCATCACAGTTTGATTAGAATCAGCCTCAAGA
GCTGGCACATGCTTGTGAACTTGTGCTTTCATATTTTCCTAACCTGTGTGGTCTTTGTGGGAGGAATAAC
CCAGACTAGGAATGCCAGCATCTGCCAAGCAGTTGGGATAATTCTTCACTATTCCACCCTTGCCACAGTA
CTATGGGTAGGAGTGACAGCTCGAAATATCTACAAACAAGTCACTAAAAAAGCTAAAAGATGCCAGGATC
CTGATGAACCACCACCTCCACCAAGACCAATGCTCAGATTTTACCTGATTGGTGGTGGTATCCCCATCAT
TGTTTGCGGCATAACTGCAGCAGCGAACATTAAGAATTACGGCAGTCGGCCAAACGCACCCTATTGCTGG
ATGGCATGGGAACCCTCCTTGGGAGCCTTCTATGGGCCAGCCAGCTTCATCACTTTTGTAAACTGCATGT
ACTTTCTGAGCATATTTATTCAGTTGAAAAGACACCCTGAGCGCAAATATGAGCTTAAGGAGCCCACGGA
GGAGCAACAGAGATTGGCAGCCAATGAAAATGGCGAAATAAATCATCAGGATTCAATGTCTTTGTCTCTG
ATTTCTACATCAGCCTTGGAAAATGAGCACACTTTTCATTCTCAGCTCTTGGGGGCCAGCCTTACTTTGC
TCTTATATGTTGCACTGTGGATGTTTGGGGCTTTGGCTGTTTCTTTGTATTACCCTTTGGACTTGGTTTT
TAGCTTCGTTTTTGGAGCCACAAGTTTAAGCTTCAGTGCGTTCTTCGTGGTCCACCATTGTGTTAATAGG
GAGGATGTTAGACTTGCGTGGATCATGACTTGCTGCCCAGGACGGAGCTCGTATTCAGTGCAAGTCAACG
TCCAGCCCCCCAACTCTAATGGGACGAATGGAGAGGCACCCAAATGCCCCAATAGCAGTGCGGAGTCTTC
ATGCACAAACAAAAGTGCTTCAAGCTTCAAAAATTCCTCCCAGGGCTGCAAATTAACAAACTTGCAGGCG
GCTGCAGCTCAGTGCCATGCCAATTCTTTACCTTTGAACTCCACCCCTCAGCTTGATAATAGTCTGACAG
AACATTCAATGGACAATGATATTAAAATGCACGTGGCGCCTTTAGAAGTTCAGTTTCGAACAAATGTGCA
CTCAAGCCGCCACCATAAAAACAGAAGTAAAGGACACCGGGCAAGCCGACTCACAGTCCTGAGAGAATAT
GCCTACGATGTCCCAACGAGCGTGGAAGGAAGCGTGCAGAACGGCTTACCTAAAAGCCGGCTGGGCAATA
ACGAAGGACACTCGAGGAGCCGAAGAGCTTATTTAGCCTACAGAGAGAGACAGTACAACCCACCCCAGCA
AGACAGCAGCGATGCTTGTAGCACACTTCCCAAAAGTAGCAGAAATTTTGAAAAGCCAGTTTCAACCACT
AGTAAAAAAGATGCGTTAAGGAAGCCAGCTGTGGTTGAACTTGAAAATCAGCAAAAATCTTATGGCCTCA
ACTTGGCCATTCAGAATGGACCAATTAAAAGCAATGGGCAGGAGGGACCCTTGCTCGGTACCGATAGCAC
TGGCAATGTTAGGACTGGATTATGGAAACACGAAACTACTGTGTAA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Ann Rev Biochem 56: 615 (1987)
2. Genome Biol. 2: S3013 (2001)
3. Science 301: 361 (2003)
4. Physiol Rev 85: 1159 (2005)
5. Genomics 88: 263 (2006)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.