Human LINGO3/LERN2/LRRN6B Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA
LINGO3 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 3) is also known as LERN2 (Leucine-rich repeat neuronal protein 2) and LRRN6B (Leucine-rich repeat neuronal protein 6B). LINGO3 is a single-pass type I transmembrane protein with an extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain.
Gene Symbol: LINGO3; LERN2; LRRN6B
NCBI Gene ID: 645191
Uniprot Entry: P0C658
Construct Details: Full length human LINGO3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 1779 (bp)
Gene Insert Sequence:
ATGACCTGCTGGCTGTGCGTCCTGAGCCTGCCCCTGCTCCTGCTGCCCGCGGCGCCGCCCCCGGCTGGAG
GCTGCCCGGCCCGCTGCGAGTGCACCGTGCAGACCCGCGCGGTGGCCTGCACGCGCCGCCGCCTGACCGC
CGTGCCCGACGGCATCCCGGCCGAGACCCGCCTGCTGGAGCTCAGCCGCAACCGCATCCGCTGCCTGAAC
CCGGGCGACCTGGCCGCGCTGCCCGCGCTGGAGGAGCTGGACCTGAGCGAGAACGCCATCGCGCACGTGG
AGCCCGGCGCCTTCGCCAACCTGCCGCGCCTGCGCGTCCTGCGTCTCCGTGGCAACCAGCTGAAGCTCAT
CCCGCCCGGGGTCTTCACGCGCCTGGACAACCTCACGCTGCTGGACCTGAGCGAGAACAAGCTGGTAATC
CTGCTGGACTACACTTTCCAGGACCTGCACAGCCTGCGCCGGCTGGAAGTGGGCGACAACGACCTGGTAT
TCGTCTCGCGCCGCGCCTTCGCGGGGCTGCTGGCCCTGGAGGAGCTGACCCTGGAGCGCTGCAACCTCAC
GGCTCTGTCCGGGGAGTCGCTGGGCCATCTGCGCAGCCTGGGCGCCCTGCGGCTGCGCCACCTGGCCATC
GCCTCCCTGGAGGACCAGAACTTCCGCAGGCTGCCCGGGCTGCTGCACCTGGAGATTGACAACTGGCCGC
TGCTGGAGGAGGTGGCGGCGGGCAGCCTGCGGGGCCTGAACCTGACCTCGCTGTCGGTCACCCACACCAA
CATCACCGCCGTGCCGGCCGCCGCGCTGCGGCACCAGGCGCACCTCACCTGCCTCAATCTGTCGCACAAC
CCCATCAGCACGGTGCCGCGGGGGTCGTTCCGGGACCTGGTCCGCCTGCGCGAGCTGCACCTGGCCGGGG
CCCTGCTGGCTGTGGTGGAGCCGCAGGCCTTCCTGGGCCTGCGCCAGATCCGCCTGCTCAACCTCTCCAA
CAACCTGCTCTCCACGTTGGAGGAGAGCACCTTCCACTCGGTGAACACGCTAGAGACGCTGCGCGTGGAC
GGGAACCCGCTGGCCTGCGACTGTCGCCTGCTGTGGATCGTGCAGCGTCGCAAGACCCTCAACTTCGACG
GGCGGCTGCCGGCCTGCGCCACCCCGGCCGAGGTGCGCGGCGACGCGCTGCGAAACCTGCCGGACTCCGT
GCTGTTCGAGTACTTCGTGTGCCGCAAACCCAAGATCCGGGAGCGGCGGCTGCAGCGCGTCACGGCCACC
GCGGGCGAAGACGTCCGCTTCCTCTGCCGCGCCGAGGGCGAGCCGGCGCCCACCGTGGCCTGGGTGACCC
CCCAGCACCGGCCGGTGACGGCCACCAGCGCGGGCCGGGCGCGCGTGCTCCCCGGGGGGACGCTGGAGAT
CCAGGACGCGCGGCCGCAGGACAGCGGCACCTACACGTGCGTGGCCAGCAACGCGGGCGGCAACGACACC
TACTTCGCCACGCTGACCGTGCGCCCCGAGCCGGCCGCCAACCGGACCCCGGGCGAGGCCCACAACGAGA
CGCTGGCGGCCCTGCGCGCGCCGCTCGACCTCACCACCATCCTGGTGTCCACCGCCATGGGCTGCATCAC
CTTCCTGGGCGTGGTCCTCTTCTGCTTCGTGCTGCTGTTCGTGTGGAGCCGCGGCCGCGGGCAGCACAAA
AACAACTTCTCGGTGGAGTACTCCTTCCGCAAGGTGGATGGGCCGGCCGCCGCGGCGGGCCAGGGAGGCG
CGCGCAAGTTCAACATGAAGATGATCTGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
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Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.