Human MSR1/SRA/SCARA1/phSR1/CD204 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

MSR1 (macrophage scavenger receptor 1), also known as CD204 and SCARA1 (scavenger receptor class A member 1), is a single-pass, type II membrane protein.  It belongs to an expanding family of transmembrane molecules collectively referred to as scavenger receptors.  Receptors of this family bind to a series of negatively­charged/polyanionic ligands.  They recognize and internalize modified low density lipoproteins (LDLs) in the removal of many foreign substances and waste materials in the living body. Scavenger receptors can be categorized into 3 classes, and MSR1 is the class A receptors with 3 different types generated by alternative splicing. The extracellular region of MSR1 contains an α­helical coiled­coil domain, a collagen­like domain, and a cysteine­rich domain (CRD) at the C-terminus. MSR1 is a macrophage-specific trimeric transmembrane glycoprotein involved in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 (MSR-AI or SR-AI) and type 2 (MSR-AII or SR-AII) are functional receptors, which mediate the endocytosis of modified LDLs. The isoform type 3 (MSR-AIII or SR-AIII) does not internalize modified LDLs. It has an altered intracellular processing and is trapped within the endoplasmic reticulum.  Thus the isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect. MSR1 may also play important roles in macrophage growth, cell adhesion, and osteoclast differentiation.

 

 

Gene Symbol: MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1

 

NCBI Gene ID: 4481

 

Uniprot Entry: P21757

 

Construct Details: Full length human MSR1/CD204 gene (encoding UniProt #P21757) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1356 (bp)

 

Gene Insert Sequence:  

ATGGAGCAGTGGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCTGTGAAATTTGATGCTCGCTC

AATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCTTCCCTTCAAGAGAAACTGAAGTCCTTCAAAGCTGCACTGA

TTGCCCTTTACCTCCTCGTGTTTGCAGTTCTCATCCCTCTCATTGGAATAGTGGCAGCTCAACTCCTGAAGTGGGAAACG

AAGAATTGCTCAGTTAGTTCAACTAATGCAAATGATATAACTCAAAGTCTCACGGGAAAAGGAAATGACAGCGAAGAGGA

AATGAGATTTCAAGAAGTCTTTATGGAACACATGAGCAACATGGAGAAGAGAATCCAGCATATTTTAGACATGGAAGCCA

ACCTCATGGACACAGAGCATTTCCAAAATTTCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGT

ACCTTGTTTTCCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTAATAAGTTTGAATACCACATT

GCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAAATCCAAGAGAATACCTTCAAACAACAAGAGGAAATCAGTA

AATTAGAGGAGCGTGTTTACAATGTATCAGCAGAAATTATGGCTATGAAAGAAGAACAAGTGCATTTGGAACAGGAAATA

AAAGGAGAAGTGAAAGTACTGAATAACATCACTAATGATCTCAGACTGAAAGATTGGGAACATTCTCAGACCTTGAGAAA

TATCACTTTAATTCAAGGTCCTCCTGGACCCCCGGGTGAAAAAGGAGATCGAGGTCCCACTGGAGAAAGTGGTCCACGAG

GATTTCCAGGTCCAATAGGTCCTCCGGGTCTTAAAGGTGATCGGGGAGCAATTGGCTTTCCTGGAAGTCGAGGACTCCCA

GGATATGCCGGAAGGCCAGGAAATTCTGGACCAAAAGGCCAGAAAGGGGAAAAGGGGAGTGGAAACACATTAACTCCATT

TACGAAAGTTCGACTGGTCGGTGGGAGCGGCCCTCACGAGGGGAGGGTGGAGATACTCCACAGCGGCCAGTGGGGTACAA

TTTGTGACGATCGCTGGGAAGTGCGCGTTGGACAGGTCGTCTGTAGGAGCTTGGGATACCCAGGTGTTCAAGCCGTGCAC

AAGGCAGCTCACTTTGGACAAGGTACTGGTCCAATATGGCTGAATGAAGTGTTTTGTTTTGGGAGAGAATCATCTATTGA

AGAATGTAAAATTCGGCAATGGGGGACAAGAGCCTGTTCACATTCTGAAGATGCTGGAGTCACTTGCACTTTATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Proc. Natl. Acad. Sci. 87: 9133 (1990).

2. J. Biol. Chem. 268:2120(1993).

3. J. Lipid Res. 34:983 (1993). 

4. J. Clin. Invest. 108:649 (2001). 

5. Cancer. Res. 63: 3486 (2003).

6. J. Immunol. 173: 6427 (2004).

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTP2291-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.